Weld joint characteristics by nano-materials addition

The need for industries to remain competitive in the welding business, has created necessity to develop innovative processes that can exceed customer’s demand. Significant development in improving weld efficiency, during the past decades, still have their drawbacks, specifically in the weld strength properties. The recent innovative technologies have created smallest possible solid material known as nanomaterial and their introduction in welding production has improved the weld strength properties and to overcome unstable microstructures in the weld. This study utilizes a qualitative research method, to elaborate the methods of introducing nanomaterial to the weldments and the characteristic of the welds produced by different welding processes. The study mainly focuses on changes in the microstructural formation and strength properties on the welded joint and also discusses those factors influencing such improvements, due to the addition of nanomaterials. The effect of nanomaterial addition in welding process modifies the physics of joining region, thereby, resulting in significant improvement in the strength properties, with stable microstructure in the weld. The addition of nanomaterials in the welding processes are, through coating on base metal, addition in filler metal and utilizing nanostructured base metal. However, due to its insignificant size, the addition of nanomaterials directly to the weld, would poses complications. The factors having major influence on the joint integrity are dispersion of nanomaterials, characteristics of the nanomaterials, quantity of nanomaterials and selection of nanomaterials. The addition of nanomaterials does not affect the fundamental properties and characteristics of base metals and the filler metal. However, in some cases, the addition of nanomaterials lead to the deterioration of the joint properties by unstable microstructural formations. Still research are ongoing to achieve high joint integrity, in various materials through different welding processes and also on other factors that influence the joint strength.

Coolability of porous core debris beds : Effects of bed geometry and multi-dimensional flooding

This thesis addresses the coolability of porous debris beds in the context of severe accident management of nuclear power reactors. In a hypothetical severe accident at a Nordic-type boiling water reactor, the lower drywell of the containment is flooded, for the purpose of cooling the core melt discharged from the reactor pressure vessel in a water pool. The melt is fragmented and solidified in the pool, ultimately forming a porous debris bed that generates decay heat. The properties of the bed determine the limiting value for the heat flux that can be removed from the debris to the surrounding water without the risk of re-melting. The coolability of porous debris beds has been investigated experimentally by measuring the dryout power in electrically heated test beds that have different geometries. The geometries represent the debris bed shapes that may form in an accident scenario. The focus is especially on heap-like, realistic geometries which facilitate the multi-dimensional infiltration (flooding) of coolant into the bed. Spherical and irregular particles have been used to simulate the debris. The experiments have been modeled using 2D and 3D simulation codes applicable to fluid flow and heat transfer in porous media. Based on the experimental and simulation results, an interpretation of the dryout behavior in complex debris bed geometries is presented, and the validity of the codes and models for dryout predictions is evaluated. According to the experimental and simulation results, the coolability of the debris bed depends on both the flooding mode and the height of the bed. In the experiments, it was found that multi-dimensional flooding increases the dryout heat flux and coolability in a heap-shaped debris bed by 47–58% compared to the dryout heat flux of a classical, top-flooded bed of the same height. However, heap-like beds are higher than flat, top-flooded beds, which results in the formation of larger steam flux at the top of the bed. This counteracts the effect of the multi-dimensional flooding. Based on the measured dryout heat fluxes, the maximum height of a heap-like bed can only be about 1.5 times the height of a top-flooded, cylindrical bed in order to preserve the direct benefit from the multi-dimensional flooding. In addition, studies were conducted to evaluate the hydrodynamically representative effective particle diameter, which is applied in simulation models to describe debris beds that consist of irregular particles with considerable size variation. The results suggest that the effective diameter is small, closest to the mean diameter based on the number or length of particles.

Upkonvertoivien nanopartikkelien pinnan muokkausmenetelmät ja niiden vaikutus partikkelien stabiilisuuteen ja luminesenssiominaisuuksiin Heterogeeninen upkonvertoiviin nanopartikkeleihin perustuva immunomääritys sydänspesifiselle troponiini I:lle

Upkonvertoivat nanopartikkelit (engl. upconverting nanoparticle, UCNP) ovat lantanidi-ioneja sisältäviä epäorgaanisia fluoroforeja, jotka muuttavat virityksessä käytettävän lähi-infrapunavalon korkeampienergiseksi emissiovaloksi. Tämän ominaisuuden ansiosta autofluoresenssi ei häiritse upkonversio-luminesenssin mittausta biologisissa sovelluksissa. Koska sovellukset edellyttävät vesiympäristöä, pinnoitteen tulee suojata UCNP:eita riittävästi veden aiheuttamalta upkonversioluminesenssin sammutukselta. Tutkielman kirjallisessa osassa esitetään erilaisia UCNP:ien pinnan muokkausmenetelmiä, ja käsitellään niiden vaikutuksia UCNP:ien stabiilisuuteen ja luminesenssi-ominaisuuksiin. Sydänperäinen troponiini I (engl. cardiac troponin I, cTnI) on sydäninfarktiin yhdistetty merkkiaine, jonka havaitseminen immunomäärityksellä auttaa sydäninfarktin diagnosointia. Tutkielman kokeellisen osan tarkoituksena oli kehittää UCNP-leimaan perustuva herkkä cTnI-immunomääritys, jossa voidaan käyttää yksinkertaista mittalaitetta. Tutkimus sisälsi kolme vaihetta, jotka olivat UCNP:ien pinnoitus hydrofiilisiksi, cTnI:tä tunnistavan vasta-aineen konjugoiti kovalenttisesti UCNP:ien pintaan ja heterogeeninen UCNP-leimaa käyttävä kaksipuolinen cTnI-immunomääritys. Eri vaiheissa käytettyjä menetelmiä optimoitiin UCNP-leiman epäspesifisen sitoutumisen vähentämiseksi ja cTnI-immunomäärityksen analyyttisen herkkyyden parantamiseksi. Immunomäärityksen toimivuutta testattiin, ja menetelmää muokattiin, jotta sillä voitaisiin määrittää myös tunnetut cTnI-pitoisuudet terveiden henkilöiden plasmanäytteistä. Poly(akryylihapolla) pinnoitetulla UCNP-leimalla määritettiin parempi analyyttinen herkkyys kuin silikalla pinnoitetulla UCNP-leimalla. Muokatun cTnI-immunomäärityksen analyyttinen herkkyys oli 2,2 ng/l. Tutkimus osoitti, että UCNP-leimalla on mahdollista havaita erittäin alhaisia cTnI-pitoisuuksia. Plasmanäytteiden cTnI-saannoissa oli eroja valituilla määritysolosuhteilla, joten toimiva immunomääritys vaatii vielä olosuhteiden lisäoptimointia.

Detection, Identification and Molecular Variation of Human Enteroviruses

Picornaviruses are the most common human viruses and the identification of the picornaviruses is nowadays based on molecular techniques, for example, reverse transcriptase polymerase chain reaction (RT-PCR). One aim of this thesis was to improve the identification of picornaviruses, especially rhino- and enteroviruses, with a real-time assay format and, also, to improve the differentiation of the viruses with genus-specific locked nucleic acid (LNA) probes. Another aim was to identify and study the causative agent of the enterovirus epidemics that appeared in Finland during seasons 2008-2010. In this thesis, the first version of picornavirus qRT-PCR with a melting curve analysis was used in a study of rhinovirus transmission within families with a rhinovirus positive index child where rhinovirus infection was monitored in all family members. In conclusion, rhinoviruses spread effectively within families causing mostly symptomatic infections in children and asymptomatic infections in adults. To improve the differentiation between rhino- and enterovirus the picornavirus qRT-PCR was modified with LNA-incorporated probes. The LNA probes were validated with picornavirus prototypes and different clinical specimen types. The LNA probe-based picornavirus qRT-PCR was able to differentiate all rhino- and enteroviruses correctly, which makes it suitable for diagnostic use. Moreover, in this thesis enterovirus outbreaks were studied with a well-observed method to create a strain-specific qRT-PCR from the typing region VP1 protein. In a hand-foot-and-mouth-disease (HFMD) outbreak in 2008, the causative agent was identified as CV-A6 and when the molecular evolution of the new HFMD CV-A6 strain was studied it was found that CV-A6 was the emerging agent for HFMD and onychomadesis. Furthermore, unusual E-30 meningitis epidemics that apeared during seasons 2009 and 2010 were studied with strain-specific qRT-PCR. The E-30 affected mostly adolescents and was probably spread in sports teams.

Recognition of nucleic acids by metal ion complexes

Metal-ion-mediated base-pairing of nucleic acids has attracted considerable attention during the past decade, since it offers means to expand the genetic code by artificial base-pairs, to create predesigned molecular architecture by metal-ion-mediated inter- or intra-strand cross-links, or to convert double stranded DNA to a nano-scale wire. Such applications largely depend on the presence of a modified nucleobase in both strands engaged in the duplex formation. Hybridization of metal-ion-binding oligonucleotide analogs with natural nucleic acid sequences has received much less attention in spite of obvious applications. While the natural oligonucleotides hybridize with high selectivity, their affinity for complementary sequences is inadequate for a number of applications. In the case of DNA, for example, more than 10 consecutive Watson-Crick base pairs are required for a stable duplex at room temperature, making targeting of sequences shorter than this challenging. For example, many types of cancer exhibit distinctive profiles of oncogenic miRNA, the diagnostics of which is, however, difficult owing to the presence of only short single stranded loop structures. Metallo-oligonucleotides, with their superior affinity towards their natural complements, would offer a way to overcome the low stability of short duplexes. In this study a number of metal-ion-binding surrogate nucleosides were prepared and their interaction with nucleoside 5´-monophosphates (NMPs) has been investigated by 1H NMR spectroscopy. To find metal ion complexes that could discriminate between natural nucleobases upon double helix formation, glycol nucleic acid (GNA) sequences carrying a PdII ion with vacant coordination sites at a predetermined position were synthesized and their affinity to complementary as well as mismatched counterparts quantified by UV-melting measurements.

Vakavat reaktorionnettomuudet ja niiden mallintaminen Olkiluoto 1 ja Olkiluoto 2 laitosyksiköille MELCOR-ohjelman versiolla 2.1

Vakaviin reaktorionnettomuuksiin liittyviä ilmiöitä on tutkittu jo 1980-luvulta lähtien ja tutkitaan edelleen. Ilmiöt liittyvät reaktorisydämen ja muiden paineastian sisäisten materi-aalien sulamiseen sekä reagointiin veden ja höyryn kanssa. Ilmiöt on myös tärkeää tuntea ja niiden esiintymistä mallintaa käytössä olevilla laitoksilla, jotta voidaan varmistua turval-lisuusjärjestelmien riittävyydestä. Olkiluoto 1 ja 2 laitosten käyttölupa uusitaan vuoteen 2018 mennessä. Lupaprosessiin liit-tyy analyysejä, joissa mallinnetaan laitosten toimintaa vakavassa reaktorionnettomuudessa. Näiden analyysien tekoon Teollisuuden Voima Oyj on käyttänyt ohjelmaa nimeltä MEL-COR jo vuodesta 1994 lähtien. Käytössä on ollut useita eri ohjelmaversioita ja viimeisin niistä on 1.8.6, joka riittää vielä tulevan käyttöluvan uusintaprojektiin liittyvien analyysien tekoon. MELCOR:n vanhaa 1.8.6 ohjelmaversioita ei kuitenkaan enää päivitetä, joten siirtyminen uudempaan 2.1 versioon on tulevaisuudessa välttämätöntä. Uusimman versiopäivityksen yhteydessä on kuitenkin muuttunut koko ohjelman lähdekoodi ja vanhojen laitosmallien käyttö uudessa ohjelmaversiossa vaatii tiedostojen konvertoinnin. Tässä työssä esitellään MELCOR-version 2.1 ominaisuuksia ja selvitetään, mitä 1.8.6 versioon luotujen laitosmal-lien käyttöönotto versiossa 2.1 vaatii. Vaatimusten määrittelemiseksi laitosmalleilla tehdään ajoja molemmilla ohjelmaversioilla ja erilaisilla onnettomuuden alkutapahtuman määrittelyillä. Tulosten perusteella arvioidaan ohjelmaversioiden eroja ja pohditaan mitä puutteita laitosmalleihin konversion jälkeen jää. Näiden perusteella arvioidaan mitä jatkotoimenpiteitä konversio vaatii.