Models to study intermediate filament dynamics and functions

Intermediate filaments are part of the cytoskeleton and nucleoskeleton; they provide cells with structure and have important roles in cell signalling. The IFs are a large protein family with more than 70 members; each tightly regulated and expressed in a cell type-specific manner. Although the IFs have been known and studied for decades, our knowledge about their specific functions is still limited, despite the fact that mutations in IF genes cause numerous severe human diseases. In this work, three IF proteins are examined more closely; the nuclear lamin A/C and the cytoplasmic nestin and vimentin. In particular the regulation of lamin A/C dynamics, the role of nestin in muscle and body homeostasis as well as the functions and evolutionary aspects of vimentin are investigated. Together this data highlights some less well understood functions of these IFs. We used mass-spectrometry to identify inter-phase specific phosphorylation sites on lamin A. With the use of genetically engineered lamin A protein in combination with high resolution microscopy and biochemical methods we discovered novel roles for this phosphorylation in regulation of lamin dynamics. More specifically, our data suggests that the phosphorylation of certain amino acids in lamin A determines the localization and dynamics of the protein. In addition, we present results demonstrating that lamin A regulates Cdk5-activity. In the second study we use mice lacking nestin to gain more knowledge of this seldom studied protein. Our results show that nestin is essential for muscle regeneration; mice lacking nestin recover more slowly from muscle injury and show signs of spontaneous muscle regeneration, indicating that their muscles are more sensitive to stresses and injury. The absence of nestin also leads to decreased over-all muscle mass and slower body growth. Furthermore, nestin has a role in controlling testicle homeostasis as nestin-/- male mice show a greater variation in testicle size. The common fruit fly Drosophila melanogaster lacks cytoplasmic IFs as most insects do. By creating a fly that expresses human vimentin we establish a new research platform for vimentin studies, as well as provide a new tool for the studies of IF evolution.

The functional fit between collaborative software and work systems:Qualification of work system needs to software functionality

The study develops an approach that tries to validate software functionality to work systems needs in SMEs. The formulated approach is constructed by using a SAAS based software i.e., work collaboration service (WCS), and SMEs as the elements of study. Where the WCS’s functionality is qualified to the collaboration needs that exist in operational and project work within SMEs. For this research constructivist approach and case study method is selected because the nature of the current study requires an in depth study of the work collaboration service as well as a detailed study of the work systems within different enterprises. Four different companies are selected in which fourteen interviews are conducted to gather data pertaining. The work systems method and framework are used as a central part of the approach to collect, analyze and interpret the enterprises work systems model and the underlying collaboration needs on operational and project work. On the other hand, the functional model of the WCS and its functionality is determined from functional model analysis, software testing, documentation and meetings with the service vendor. The enterprise work system model and the WCS model are compared to reveal how work progression differs between the two and make visible unaddressed stages of work progression. The WCS functionality is compared to work systems collaboration needs to ascertain if the service will suffice the needs of the project and operational work under study. The unaddressed needs provide opportunities to improve the functionality of the service for better conformity to the needs of enterprise and work. The results revealed that the functional models actually differed in how operational and project work progressed within the stages. WCS shared similar stages of work progression apart from the stages of identification and acceptance, and progress and completion stages were only partially addressed. Conclusion is that the identified unaddressed needs such as, single point of reference, SLA and OLA inclusion etc., should be implemented or improved within the WCS at appropriate stages of work to gain better compliance of the service to the needs of the enterprise an work itself. The developed approach can hence be used to carry out similar analysis for the conformance of pre-built software functionality to work system needs with SMEs.

The New Silk Road, a Glimpse to China's Regime?

China's Silk Road Economic Belt plan is a part of One Belt, One Road initiative that aims to create trade routes from China all the way to Europe. Despite the potential benefits, there are also problems along the way. In this research I am examining the adverse effects of one part of the Silk Road Economic Belt with my focus on Xinjiang Uyghur minority and their rights and Central Asian regional stability. Moreover, I suggest that China's past commitments in the international society as well as her actions in relations to the undertaking can give an insight into a regime where China would be the dominant power in international society. I have used qualitative analysis to study the topics. My most important methodological tools to examine the topics are as follows. I utilise conceptual analysis to borrow concepts from international relations field. I use method of situation analysis when I am describing the current circumstances in China's Xinjiang and Central Asia. Inductive analysis is the overall method since I suggest that the content I have examined could give an insight to how China regards and relates to international law in the future. Moreover, my theoretical framework of the research sees international law as a tool that a state can use to gain more power but at the same time international law restricts state's behaviour. Based on the findings of this research, in case of Xinjiang the New Silk Road is likely to worsen Uyghurs situation because of Beijing's worries and harsh actions to prevent any disturbance. However, the New Silk Road could bring stability and maintain regional security in Central Asia when the states could see it beneficial to unite for cooperation which can result with greater benefits. China's potential future regime will emphasize sovereignty and non-interference to states’ domestic matters. Moreover, there will be no room for minority rights in China's concept of human rights. Human rights are meant to protect rights of masses but are of secondary importance since development and security will be more important goals to pursue. In the field of cooperation, China is increasingly using multilateral forums to discuss the matters but reserves bilateral negotiations for executing the plans.