NADPH-Dependent Thioredoxin System In Regulation of Chloroplast Functions

Photosynthesis, the process in which carbon dioxide is converted into sugars using the energy of sunlight, is vital for heterotrophic life on Earth. In plants, photosynthesis takes place in specific organelles called chloroplasts. During chloroplast biogenesis, light is a prerequisite for the development of functional photosynthetic structures. In addition to photosynthesis, a number of other metabolic processes such as nitrogen assimilation, the biosynthesis of fatty acids, amino acids, vitamins, and hormones are localized to plant chloroplasts. The biosynthetic pathways in chloroplasts are tightly regulated, and especially the reduction/oxidation (redox) signals play important roles in controlling many developmental and metabolic processes in chloroplasts. Thioredoxins are universal regulatory proteins that mediate redox signals in chloroplasts. They are able to modify the structure and function of their target proteins by reduction of disulfide bonds. Oxidized thioredoxins are restored via the action of thioredoxin reductases. Two thioredoxin reductase systems exist in plant chloroplasts, the NADPHdependent thioredoxin reductase C (NTRC) and ferredoxin-thioredoxin reductase (FTR). The ferredoxin-thioredoxin system that is linked to photosynthetic light reactions is involved in light-activation of chloroplast proteins. NADPH can be produced via both the photosynthetic electron transfer reactions in light, and in darkness via the pentose phosphate pathway. These different pathways of NADPH production enable the regulation of diverse metabolic pathways in chloroplasts by the NADPH-dependent thioredoxin system. In this thesis, the role of NADPH-dependent thioredoxin system in the redox-control of chloroplast development and metabolism was studied by characterization of Arabidopsis thaliana T-DNA insertion lines of NTRC gene (ntrc) and by identification of chloroplast proteins regulated by NTRC. The ntrc plants showed the strongest visible phenotypes when grown under short 8-h photoperiod. This indicates that i) chloroplast NADPH-dependent thioredoxin system is non-redundant to ferredoxinthioredoxin system and that ii) NTRC particularly controls the chloroplast processes that are easily imbalanced in daily light/dark rhythms with short day and long night. I identified four processes and the redox-regulated proteins therein that are potentially regulated by NTRC; i) chloroplast development, ii) starch biosynthesis, iii) aromatic amino acid biosynthesis and iv) detoxification of H₂O₂. Such regulation can be achieved directly by modulating the redox state of intramolecular or intermolecular disulfide bridges of enzymes, or by protecting enzymes from oxidation in conjunction with 2-cysteine peroxiredoxins. This thesis work also demonstrated that the enzymatic antioxidant systems in chloroplasts, ascorbate peroxidases, superoxide dismutase and NTRC-dependent 2-cysteine peroxiredoxins are tightly linked up to prevent the detrimental accumulation of reactive oxygen species in plants.

Strategic communication : practice, ideology and dissonance

This dissertation approaches the manifestations of ideology in U.S. Strategic Communication. The discussion approaches Strategic Communication by relating it to the Enlightenment narratives and suggesting these narratives maintain similar social and political functions. This dissertation aims to address the key contents and mechanisms of Strategic Communication by covering the perspectives of (i) communication as leadership as well as (ii) communication as discourse , i.e. practice and contents. Throughout the empirical part of the dissertation, the communication theoretical discussion is supported by a methodological framework that bridges Critical Discourse Analysis (CDA) and functional language theory. According to the principles of CDA, Strategic Communication is treated as ideological, hegemonic discourse that impacts social order. The primary method of analysis is transitivity analysis, which is concerned with how language and its patterns construe reality. This analysis is complemented with a discussion on the rituals of production and interpretation, which can be treated as visual extensions of textual transitivity. The concept of agency is the key object of analysis. From the perspective of leadership, Strategic Communication is essentially a leadership model through which the organization defines itself, its aims and legitimacy. This dissertation arrives to the conclusion that Strategic Communication is used not only as a concept for managing Public Relations and information operations. It is an esse ntial asset in the inter-organization management of its members. The current developments indicate that the concept is developing towards even heavier measures of control. From the perspective of language and discourse, the key narratives of Strategic Communication are advocated with the intrinsic values of democracy and technological progress as the prerequisites of ethics and justice. The transitivity patterns reveal highly polarized agency. The agency of the Self is typically outsourced to technology. Further, the transitivity pa tterns demonstrate how the effects-centric paradigm of warfare has created a lexicon that is ideologically exclusive. It has led to the development of two mutually exclusive sets of vocabulary, where the desc riptions of legitimate ac tion exclude Others by default. These ideological discourses have become naturalized in the official vocabulary of strategic planning and le adership. Finally, the analysis of the images of the captures and deaths of Saddam Hussein, Osama bin Laden and Muammar Gaddafi bring the discussion back to the themes of the Enlightenment by demonstrating how democracy is framed to serve political purposes. The images of democracy are essentially images of violence. Contrary to the official, instrumental and humanitari an narratives of Strategic Communication, it is the grammar of expressive, violent rituals that serve as the instrument of unity.

Ristikkorakenteisen hihnakuljetinsillan mitoitustyökalun kehittäminen

Hihnakuljettimia käytetään muun muassa voimalaitos- ja sellutehdasympäristöissä kuljettamaan kiinteää polttoainetta tai haketta pitkiä matkoja. Pitkät hihnakuljettimet asennetaan yleensä teräsristikoista rakennettujen kuljetinsiltojen sisään. Kuljetinsilta toimii siis hihnakuljettimen ja hoitokäytävän runkona sekä suojaa kuljetinta ja kuljetettavaa materiaalia säältä. Tässä diplomityössä on laadittu mitoitustyökalu, jolla voidaan nopeasti mitoittaa ristikkorakenteisen hihnakuljetinsillan sauvat sekä määrittää rakenteen massa. Laskentaohjelma on toteutettu Microsoft Excel -taulukkolaskentana. Sillan poikkileikkaus voi olla suljettu symmetrinen umpirakenne tai avorakenne, jossa on ulokkeellinen hoitotaso. Ristikon sauvat ovat RHS-putkia. Rakenteeseen voi vaikuttaa jatkuvia kuormia, pistekuormia sekä näistä johdetut, ekvivalenttiin staattiseen voimaan perustuvat maanjäristyskuormat. Diplomityössä on perehdytty mitoitustyökalussa sovellettuihin teorioihin. Voimasuureiden laskenta perustuu 2-tukisina palkkeina käsiteltävien siltalohkojen ratkaisuun ja palkkianalogiaan, jossa ristikon sauvavoimat ratkaistaan käsittelemällä ristikkoa palkkina. Sauvojen kestävyyden laskenta perustuu SFS-EN 1993-1-1 -normiin, joka on osa Eurocode -rakennesuunnittelunormistoa. Lisäksi työssä on käsitelty ristikkoliitosten mitoitusta SFS-EN 1993-1-8 mukaan. Mitoitusohjelman toimivuutta on testattu tarkastelemalla esimerkkisiltaa laaditulla mitoituspohjalla ja Autodesk Robot Professional 2013 -FE-analyysiohjelmisuolla. Tulosten perusteella mitoitustyökalua voidaan käyttää ainakin tarjousvaiheen nopeisiin tarkasteluihin ja omamassan määritykseen, mutta myös lopulliseen mitoitukseen, mikäli hyväksytään konservatiivinen mitoitus.

Hitsattavan konstruktion laadun ja rakenteen kehittäminen lyöntipaalutuskoneessa

Työn tavoitteena oli Junttan Oy:n valmistaman lyöntipaalutuskoneeseen kuuluvan tärkeän konstruktion, peruskeilin, laadun ja rakenteen kehittäminen. Työ tehtiin osana Tekesin ja usean yrityksen rahoittamaa HitNet-projektia (Hitsaavan teollisuuden hankintatoimen ja toimitusketjun tehostaminen). Peruskeiliä tutkittiin seuraamalla sen valmistusta Junttan Oy:n omassa tuotannossa sekä alihankintayrityksissä. Ongelmana peruskeilin suunnittelussa ja valmistuksessa olivat hyvin tarkat toleranssirajat sen liukupinnoille. Suurimmat ongelmat syntyivät hitsaamisen tuoman suuren lämmöntuonnin aiheuttamien muodonmuutosten ja särmäyksestä johtuvan takaisinjouston yhteydessä. Näihin ongelmiin pyrittiin kehittämään ratkaisuja peruskeilin rakennetta muuttamalla, Total Welding Management -laatutyökalua hyödyntämällä sekä hitsaustoimintaa ja särmäystä kehittämällä. Peruskeilin suunnittelussa ja valmistuksessa havaittujen ongelmien perusteella kehitettiin useita ratkaisuja, joista yksi otettiin käyttöön ja siitä valmistetaan prototyyppi Junttan Oy:n omassa tuotannossa. Uuden mallin valmistuessa päätetään tuleeko siitä korvaaja edeltäjälleen vai testataanko vielä muita kehitysideoita.

Siltojen korjauskustannukset ja niihin vaikuttavat tekijät

Työn tavoitteena oli tunnistaa merkittävimmät sillankorjauskustannuksiin vaikuttavat tekijät, ja selvittää niiden vaikutusten suuruus asiantuntijahaastattelujen ja ELY-keskuksista saatavien kustannustietojen yksikköhintaluetteloiden avulla. Kaakkois-Suomen, Pirkanmaan, Pohjois-Savon, Varsinais-Suomen ja Uudenmaan ELY-keskuksista saatiin yhteensä 37 sillankorjausurakan kustannustiedot. Lisäksi käytössä oli Vt6 Lappeenranta-Imatra ja Vt7 Haminan ohikulkutie –valtatiehankkeiden kustannustiedot. Urakoissa korjattiin yhteensä 131 siltaa. Kustannustietojen ja asiantuntijahaastatteluiden perusteella tunnistettiin merkittävimmät kustannuksiin vaikuttavat tekijät, ja saatiin luotua arvio niiden vaikutuksille eri tapauksissa. Lisäksi saatiin selvitettyä mahdollisuuksia alentaa korjauskustannuksia, ja luotiin toimintamalli jolla saadaan toteutettua korjausurakka mahdollisimman alhaisilla kustannuksilla.

Konepajan hitsaustuotannon kehittäminen kattavien laatuvaatimusten mukaiseksi

TIIVISTELMÄ Lappeenrannan teknillinen yliopisto Konetekniikan koulutusohjelma Voitto Kettunen Konepajan hitsaustuotannon kehittäminen kattavien laatuvaatimusten mukaiseksi Diplomityö 2015 167 sivua, 39 kuvaa, 26 taulukkoa ja 3 liitettä Tarkastajat: Professori Jukka Martikainen DI Pertti Kaarre Hakusanat: hitsaus, hitsauksen laatu, konepajan laadunhallinta, kattavat laatuvaatimukset, ISO 9001, ISO 3834, EN 1090 Keywords: welding, quality of welding, engineering workshop quality management, comprehensive quality requirements, ISO 9001, ISO 3834, EN 1090 Hitsaamalla liitetyt teräksiset rakenteet muodostavat ylivoimaisesti suurimman osan konepajatuotannosta. Niihin kuuluu esimerkiksi ajoneuvoja, koneita, laitteita, säiliöitä, siiloja, siltoja, mastoja, piippuja, tukirakenteita ja rakennusten runkoja. Tämän diplomityön tavoitteena on kehittää konepajan laadunhallinta sellaiseksi, että se mahdollistaa kattavien laatuvaatimusten täyttämisen hitsaustuotannossa. Laatuvaatimusten täyttämiseen pyritään käyttämällä hitsaustoimintojen standardia EN ISO 3834-2 sekä kantavien teräsrakenteiden standardeja EN 1090-1 ja EN 1090-2. Teräsrakenteiden suunnittelua ohjaa EN 1993 ja niiden toiminnallisia ominaisuuksia tuotestandardit, kuten terässavupiippu- ja säiliöstandardit. Kantavien teräsrakenteiden suunnittelua ja tuotantoa ohjaa myös seuraamusluokan CC, käyttöluokan SC ja tuotantoluokan PC kautta määräytyvä toteutusluokka EXC. Aikaisempaa enemmän tullaan panostamaan esimerkiksi asiakirjojen sähköiseen hallintaan, raaka-aineiden jäljitettävyyteen tuotteeseen, särmien ja kulmien muotoiluun, pintojen käsittelyyn, hitsien tarkastukseen, hitsaushenkilöstön pätevyyteen ja hitsaustuotannon tehokkuuteen. Saarijärven Säiliövalmiste Oy:n hitsauksen laadunhallinta sertifioitiin standardin ISO 3834-2 mukaan ja kantavien teräsrakenteiden FPC-järjestelmä standardisarjaa EN 1090 noudattaen. Samalla tehtiin päivitys laadunhallintajärjestelmään ISO 9001. Toteutus, joka tehtiin sovitussa aikataulussa, haastaa jokaisen toimijan konepajassa toiminnan, tuotannon ja tuotteiden laadun kehittämiseen uusia käytänteitä ja menetelmiä soveltaen. Kehitystoimien tuloksena toiminta on selkeämpää, ennakoitavampaa ja hallitumpaa, mikä lisää yrityksen toiminnan tuottavuutta ja kannattavuutta. Sertifioidut laatujärjestelmät ovat myötävaikuttaneet tilausten lisääntymiseen yrityksen kaikkien tuotteiden osalta.

Quantitative refinement of reaction-based biomodels

In the field of molecular biology, scientists adopted for decades a reductionist perspective in their inquiries, being predominantly concerned with the intricate mechanistic details of subcellular regulatory systems. However, integrative thinking was still applied at a smaller scale in molecular biology to understand the underlying processes of cellular behaviour for at least half a century. It was not until the genomic revolution at the end of the previous century that we required model building to account for systemic properties of cellular activity. Our system-level understanding of cellular function is to this day hindered by drastic limitations in our capability of predicting cellular behaviour to reflect system dynamics and system structures. To this end, systems biology aims for a system-level understanding of functional intraand inter-cellular activity. Modern biology brings about a high volume of data, whose comprehension we cannot even aim for in the absence of computational support. Computational modelling, hence, bridges modern biology to computer science, enabling a number of assets, which prove to be invaluable in the analysis of complex biological systems, such as: a rigorous characterization of the system structure, simulation techniques, perturbations analysis, etc. Computational biomodels augmented in size considerably in the past years, major contributions being made towards the simulation and analysis of large-scale models, starting with signalling pathways and culminating with whole-cell models, tissue-level models, organ models and full-scale patient models. The simulation and analysis of models of such complexity very often requires, in fact, the integration of various sub-models, entwined at different levels of resolution and whose organization spans over several levels of hierarchy. This thesis revolves around the concept of quantitative model refinement in relation to the process of model building in computational systems biology. The thesis proposes a sound computational framework for the stepwise augmentation of a biomodel. One starts with an abstract, high-level representation of a biological phenomenon, which is materialised into an initial model that is validated against a set of existing data. Consequently, the model is refined to include more details regarding its species and/or reactions. The framework is employed in the development of two models, one for the heat shock response in eukaryotes and the second for the ErbB signalling pathway. The thesis spans over several formalisms used in computational systems biology, inherently quantitative: reaction-network models, rule-based models and Petri net models, as well as a recent formalism intrinsically qualitative: reaction systems. The choice of modelling formalism is, however, determined by the nature of the question the modeler aims to answer. Quantitative model refinement turns out to be not only essential in the model development cycle, but also beneficial for the compilation of large-scale models, whose development requires the integration of several sub-models across various levels of resolution and underlying formal representations.

Fiber-reinforced composite fixed dental prostheses: Studies of the materials used as pontics

Fiber-reinforced composite fixed dental prostheses – Studies of the materials used as pontics University of Turku, Faculty of Medicine, Institute of Dentistry, Department of Biomaterials Science, Finnish Doctoral Program in Oral Sciences – FINDOS, Annales Universitatis Turkuensis, Turku, Finland 2015 Fiber-reinforced composites (FRC), a non-metallic biomaterial, represent a suitable alternative in prosthetic dentistry when used as a component of fixed dental prostheses (FDPs). Some drawbacks have been identified in the clinical performance of FRC restorations, such as delamination of the veneering material and fracture of the pontic. Therefore, the current series of studies were performed to investigate the possibilities of enhancing the mechanical and physical properties of FRC FDPs by improving the materials used as pontics, to then heighten their longevity. Four experiments showed the importance of the pontic design and surface treatment in the performance of FRC FDPs. In the first, the load-bearing capacities of inlay-retained FRC FDPs with pontics of various materials and thicknesses were evaluated. Three different pontic materials were assessed with different FRC framework vertical positioning. Thicker pontics showed increased load-bearing capacities, especially ceramic pontics. A second study was completed investigating the influence of the chemical conditioning of the ridge-lap surface of acrylic resin denture teeth on their bonding to a composite resin. Increased shear bond strength demonstrated the positive influence of the pretreatment of the acrylic surfaces, indicating dissolution of the denture surfaces, and suggesting potential penetration of the monomer systems into the surface of denture teeth. A third study analyzed the penetration depth of different monomer systems on the acrylic resin denture teeth surfaces. The possibility of establishing a durable bond between acrylic pontics and FRC frameworks was demonstrated by the ability of monomers to penetrate the surface of acrylic resin denture teeth, measured by a confocal scanning type microscope. A fourth study was designed to evaluate the load-bearing capacities of FRC FDPs using the findings of the previous three studies. In this case, the performance of pre-shaped acrylic resin denture teeth used as pontics with different composite resins as filling materials was evaluated. The filling material influenced the load-bearing capacities, providing more durable FRC FDPs. It can be concluded that the mechanical and physical properties of FRC FDPs can be improved as has been shown in the development of this thesis. The improvements reported then might provide long lasting prosthetic solutions of this kind, positioning them as potentially permanent rehabilitation treatments. Key words: fiber-reinforced composite, fixed dental prostheses, inlay-retained bridges, adhesion, acrylic resin denture teeth, dental material.

Development of a Control System for a Tele Operated Mobile Robot

Mobile robots are capable of performing spatial displacement motions in different environments. This motions can be calculated based on sensorial data (autonomous robot) or given by an operator (tele operated robot). This thesis is focused on the latter providing the control architecture which bridges the tele operator and the robot’s locomotion system and end effectors. Such a task might prove overwhelming in cases where the robot comprises a wide variety of sensors and actuators hence a relatively new option was selected: Robot Operating System (ROS). The control system of a new robot will be sketched and tested in a simulation model using ROS together with Gazebo in order to determine the viability of such a system. The simulated model will be based on the projected shape and main features of the real machine. A stability analysis will be performed first theoretically and afterwards using the developed model. This thesis concluded that both the physical properties and the control architecture are feasible and stable settling up the ground for further work with the same robot.

Dynamic capabilities as enablers of value co-creation between an MBA school and its students

This study presents an understanding of how a U.S. based, international MBA school has been able to achieve competitive advantage within a relatively short period of time. A framework is built to comprehend how the dynamic capability and value co-creation theories are connected and to understand how the dynamic capabilities have enabled value co-creation to happen between the school and its students, leading to such competitive advantage for the school. The data collection method followed a qualitative single-case study with a process perspective. Seven semi-structured interviews were made in September and October of 2015; one current employee of the MBA school was interviewed, with the other six being graduates and/or former employees of the MBA school. In addition, the researcher has worked as a recruiter at the MBA school, enabling to build bridges and a coherent whole of the empirical findings. Data analysis was conducted by first identifying themes from interviews, after which a narrative was written and a causal network model was built. Thus, a combination of thematic analysis, narrative and grounded theory were used as data analysis methods. This study finds that value co-creation is enabled by the dynamic capabilities of the MBA school; also capabilities would not be dynamic if value co-creation did not take place. Thus, this study presents that even though the two theories represent different level analyses, they are intertwined and together they can help to explain competitive advantage. The MBA case school’s dynamic capabilities are identified to be the sales & marketing capabilities and international market creation capabilities, thus the study finds that the MBA school does not only co-create value with existing students (customers) in the school setting, but instead, most of the value co-creation happens between the school and the student cohorts (network) already in the recruiting phase. Therefore, as a theoretical implication, the network should be considered as part of the context. The main value created seem to lie in the MBA case school’s international setting & networks. MBA schools around the world can learn from this study; schools should try to find their own niche and specialize, based on their own values and capabilities. With a differentiating focus and a unique and practical content, the schools can and should be well-marketed and proactively sold in order to receive more student applications and enhance competitive advantage. Even though an MBA school can effectively be treated as a business, as the study shows, the main emphasis should still be on providing quality education. Good content with efficient marketing can be the winning combination for an MBA school.