1 resultado para Import
em Université de Lausanne, Switzerland
Filtro por publicador
- Aberystwyth University Repository - Reino Unido (1)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Academic Research Repository at Institute of Developing Economies (36)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (7)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (3)
- Aquatic Commons (29)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (12)
- Archive of European Integration (172)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (5)
- Aston University Research Archive (3)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (4)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (7)
- Biblioteca Valenciana Digital - Ministerio de Educación, Cultura y Deporte - Valencia - Espanha (3)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (62)
- Boston University Digital Common (1)
- Brock University, Canada (5)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- CentAUR: Central Archive University of Reading - UK (27)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (6)
- Cochin University of Science & Technology (CUSAT), India (9)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (87)
- CORA - Cork Open Research Archive - University College Cork - Ireland (2)
- Dalarna University College Electronic Archive (3)
- Deakin Research Online - Australia (52)
- Digital Archives@Colby (1)
- DigitalCommons@The Texas Medical Center (4)
- DigitalCommons@University of Nebraska - Lincoln (3)
- Digitale Sammlungen - Goethe-Universität Frankfurt am Main (4)
- Duke University (5)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (4)
- Helda - Digital Repository of University of Helsinki (9)
- Indian Institute of Science - Bangalore - Índia (19)
- Infoteca EMBRAPA (1)
- Instituto Nacional de Saúde de Portugal (1)
- Instituto Politécnico do Porto, Portugal (9)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- Memoria Académica - FaHCE, UNLP - Argentina (6)
- Ministerio de Cultura, Spain (12)
- National Center for Biotechnology Information - NCBI (31)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (11)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (25)
- Queensland University of Technology - ePrints Archive (35)
- RDBU - Repositório Digital da Biblioteca da Unisinos (1)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (4)
- Repositório digital da Fundação Getúlio Vargas - FGV (21)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositorio Institucional da UFLA (RIUFLA) (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional da Universidade Estadual de São Paulo - UNESP (3)
- Repositorio Institucional de la Universidad Nacional Agraria (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (57)
- Repositorio Institucional Universidad EAFIT - Medelin - Colombia (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Universidad de Alicante (1)
- Universidad del Rosario, Colombia (45)
- Universidad Politécnica de Madrid (11)
- Universidade Complutense de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade Federal do Pará (5)
- Universidade Federal do Rio Grande do Norte (UFRN) (8)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (4)
- Université de Lausanne, Switzerland (1)
- Université de Montréal (1)
- Université de Montréal, Canada (20)
- University of Connecticut - USA (3)
- University of Michigan (31)
- University of Southampton, United Kingdom (4)
Resumo:
Eukaryotes contain inorganic polyphosphate (polyP) and acidocalcisomes, which sequester polyP and store amino acids and divalent cations. Why polyP is sequestered in dedicated organelles is not known. We show that polyP produced in the cytosol of yeast becomes toxic. Reconstitution of polyP translocation with purified vacuoles, the acidocalcisomes of yeast, shows that cytosolic polyP cannot be imported, whereas polyP produced by the vacuolar transporter chaperone (VTC) complex, an endogenous vacuolar polyP polymerase, is efficiently imported and does not interfere with growth. PolyP synthesis and import require an electrochemical gradient, probably as a driving force for polyP translocation. VTC exposes its catalytic domain to the cytosol and carries nine vacuolar transmembrane domains. Mutations in the VTC transmembrane regions, which are likely to constitute the translocation channel, block not only polyP translocation but also synthesis. Given that they are far from the cytosolic catalytic domain of VTC, this suggests that the VTC complex obligatorily couples synthesis of polyP to its import in order to avoid toxic intermediates in the cytosol. Sequestration of otherwise toxic polyP might be one reason for the existence of acidocalcisomes in eukaryotes.