1 resultado para Daughter of the Forest
em Université de Lausanne, Switzerland
Filtro por publicador
- KUPS-Datenbank - Universität zu Köln - Kölner UniversitätsPublikationsServer (1)
- Adam Mickiewicz University Repository (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (2)
- Aquatic Commons (9)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (5)
- Archive of European Integration (8)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (4)
- Avian Conservation and Ecology - Eletronic Cientific Hournal - Écologie et conservation des oiseaux: (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (41)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (30)
- Biblioteca Valenciana Digital - Ministerio de Educación, Cultura y Deporte - Valencia - Espanha (1)
- Biodiversity Heritage Library, United States (3)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (45)
- Brock University, Canada (3)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- Cambridge University Engineering Department Publications Database (1)
- CentAUR: Central Archive University of Reading - UK (37)
- Center for Jewish History Digital Collections (11)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (20)
- Cochin University of Science & Technology (CUSAT), India (2)
- Coffee Science - Universidade Federal de Lavras (3)
- Collection Of Biostatistics Research Archive (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (3)
- CORA - Cork Open Research Archive - University College Cork - Ireland (1)
- Dalarna University College Electronic Archive (3)
- Deakin Research Online - Australia (39)
- Digital Archives@Colby (2)
- Digital Commons - Michigan Tech (10)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (2)
- DigitalCommons@The Texas Medical Center (2)
- DigitalCommons@University of Nebraska - Lincoln (3)
- Duke University (3)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (8)
- Glasgow Theses Service (1)
- Greenwich Academic Literature Archive - UK (3)
- Harvard University (4)
- Helda - Digital Repository of University of Helsinki (24)
- Indian Institute of Science - Bangalore - Índia (16)
- Instituto Politécnico de Bragança (1)
- Instituto Politécnico do Porto, Portugal (1)
- Memoria Académica - FaHCE, UNLP - Argentina (3)
- Memorial University Research Repository (1)
- National Center for Biotechnology Information - NCBI (4)
- Portal de Revistas Científicas Complutenses - Espanha (4)
- Publishing Network for Geoscientific & Environmental Data (20)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (24)
- Queensland University of Technology - ePrints Archive (27)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (2)
- Repositório Científico da Universidade de Évora - Portugal (4)
- Repositório digital da Fundação Getúlio Vargas - FGV (1)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (170)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- Universidad Autónoma de Nuevo León, Mexico (1)
- Universidad de Alicante (1)
- Universidad Politécnica de Madrid (11)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade de Madeira (1)
- Universidade Federal do Pará (5)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (2)
- Université de Lausanne, Switzerland (1)
- Université de Montréal, Canada (3)
- University of Canberra Research Repository - Australia (1)
- University of Michigan (316)
- University of Queensland eSpace - Australia (7)
- University of Washington (3)
Resumo:
Semliki Forest virus (SFV) vectors have been efficiently used for rapid high level expression of several G protein-coupled receptors. Here we describe the use of SFV vectors to express the alpha 1b-adrenergic receptor (AR) alone or in the presence of the G protein alpha q and/or beta 2 and gamma 2 subunits. Infection of baby hamster kidney (BHK) cells with recombinant SFV-alpha 1b-AR particles resulted in high specific binding activity of the alpha 1b-AR (24 pmol receptor/mg protein). Time-course studies indicated that the highest level of receptor expression was obtained 30 hours post-infection. The stimulation of BHK cells, with epinephrine led to a 5-fold increase in inositol phosphate (IP) accumulation, confirming the functional coupling of the receptor to G protein-mediated activation of phospholipase C. The SFV expression system represents a rapid and reproducible system to study the pharmacological properties and interactions of G protein coupled receptors and of G protein subunits.