Mapping cell-matrix stresses during stretch reveals inelastic reorganization of the cytoskeleton.

The mechanical properties of the living cell are intimately related to cell signaling biology through cytoskeletal tension. The tension borne by the cytoskeleton (CSK) is in part generated internally by the actomyosin machinery and externally by stretch. Here we studied how cytoskeletal tension is modified during stretch and the tensional changes undergone by the sites of cell-matrix interaction. To this end we developed a novel technique to map cell-matrix stresses during application of stretch. We found that cell-matrix stresses increased with imposition of stretch but dropped below baseline levels on stretch release. Inhibition of the actomyosin machinery resulted in a larger relative increase in CSK tension with stretch and in a smaller drop in tension after stretch release. Cell-matrix stress maps showed that the loci of cell adhesion initially bearing greater stress also exhibited larger drops in traction forces after stretch removal. Our results suggest that stretch partially disrupts the actin-myosin apparatus and the cytoskeletal structures that support the largest CSK tension. These findings indicate that cells use the mechanical energy injected by stretch to rapidly reorganize their structure and redistribute tension.

Noves funcions en un entorn de canvis: implicacions de l'accés obert per a les biblioteques

Aquest article examina les possibles implicacions de l'accés obert en la gestió de les biblioteques. Aquesta examinació té lloc en un context en què s'assumeix que el model tradicional d'edició i l'accés obert coexisteixen. L'accés obert presenta nombrosos reptes i oportunitats, però les biblioteques emprenedores trobaran noves formes per servir els usuaris en un nou entorn en el que es combina l'accés obert amb el tradicional (MOA). Per tal de portar-ho a terme, caldrà que aquestes biblioteques redissenyin la seva organització, i això podrà suposar una pressió sobre els recursos econòmics i de personal.

Condicions socioecològiques de la riera d'Arbúcies pel retorn de la llúdriga (Lutra lutra)

La llúdriga (Lutra lutra) va desaparèixer de la conca de la Tordera a causa de la pressió humana sobre ella i el seu hàbitat. Recentment les seves poblacions s’estan recuperant a las conques nord de Catalunya. En aquest context es presenta els resultats dels anàlisis dels requeriments socioecològics de la llúdriga en l’afluent del riu Tordera de la riera d’Arbúcies: qualitat del bosc de ribera, valoració de la contaminació de l’aigua i l’anàlisi de les poblacions d’ictiofauna, a més de l’estimació de l’espècie més abundant. L’evolució de l’ús i cobertes del sòl del sòl mostren que existeix una tendència augment en las masses forestals, zones urbanitzades i infraestructures; també una disminució del camps de cultiu, fruiters i vinyes. La qualitat del bosc de ribera es va valorar a partir del l’índex QBR, obtenint que la màxima qualitat es localitza en el tram alt, disminuint a mesura que s’apropa a la desembocadura al Tordera. La contaminació de l’aigua a estat valorada, per una banda analitzant la qualitat biològica a partir dels índexs IPS i BMWPC, obtenint que la qualitat de l’aigua disminueix a mesura que transcorre riu avall, segons l’IPS. Amb el BMWPC es troba que existeix una recuperació de la qualitat de l’aigua en l’últim tram. Per altra banda s’han analitzat el compostos químics que afecten a la llúdriga, obtenint que les concentracions d’aquest no són rellevants a l’aigua. S’ha analitzat l’estructura de la població d’ictiofauna present, trobant que el Barbus meridionalis és l’espècie més abundant en tots el trams, a més d’augmentar en captures a mesura que l’aigua s’apropa al aiguabarreig amb la Tordera. S’ha estimat la biomassa present d’aquesta espècie, concloent que es suficient per mantenir una població no gaire densa de llúdrigues, inferior a 0,15 individus per kilòmetre de riu.

The Value of Imperfection : the Wabi-Sabi Principle in Aesthetics and Learning

OER-based learning has the potential to overcome many shortcomings and problems of traditional education. It is not hampered by IP restrictions; can depend on collaborative, cumulative, iterative refinement of resources; and the digital form provides unprecedented flexibility with respect to configuration and delivery. The OER community is a progressive group of educators and learners with decades of learning research to draw from, who know that we must prepare learners for an evolving and diverse reality. Despite this OER tends to replicate the unsuccessful characteristics of traditional education. To remedy this we may need to remember the importance of imperfection, mistakes, problems, disagreement, and the incomplete for engaged learning, and relinquish our notions of perfection, acknowledging that learners learn differently and we need diverse learners. We must stretch our perceptions of quality and provide mechanisms for engaging the incredible pool of educators globally to fulfill the promise of inclusive education.

The Emergence and Governance of Euroregions: The Case of the Euregio on the Dutch-German Border

Amb una història que data de la dècada de 1950, la EUREGIO és un de les més antigues euroregions a Europa. Es pot considerar com un cas exitós d'una regió transfronterera (CBR) en el sentit que s'ha establert fermament com una agència de fronteres dins del seu tram de la frontera holandesa-alemanya. L'EUREGIO també ha estat una de les protagonistes principals darrere de l'Associació de Regions Frontereres Europees (ARFE), que en les últimes dècades va actuar per difondre el model d'euroregió a tot el territori europeu. Aquest capítol té diversos objectius. En primer lloc, es presenta el cas de la EUREGIO i presenta evidència sobre la seva història, estructura orgànica i polítiques. En segon lloc, s'analitzen les condicions del context en què la EUREGIO ha sorgit i les estructures de govern que es van crear com a resultat. Es fa especial èmfasi en la posició i el paper de l'Euroregió en el context més ampli del marc de governança europea multinivell generat per la política de cohesió de la UE. El capítol conclou amb un intent d'avaluar l'èxit i l'impacte de la EUREGIO i una discussió dels reptes relacionats amb la doble funció de l'EUREGIO com a representant dels interessos de les autoritats locals i les agències de cohesió de la UE posada en pràctica de les polítiques.

Low viscosity contrast fingering in a rotating Hele-Shaw cell

We study the fingering instability of a circular interface between two immiscible liquids in a radial Hele-Shaw cell. The cell rotates around its vertical symmetry axis, and the instability is driven by the density difference between the two fluids. This kind of driving allows studying the interfacial dynamics in the particularly interesting case of an interface separating two liquids of comparable viscosity. An accurate experimental study of the number of fingers emerging from the instability reveals a slight but systematic dependence of the linear dispersion relation on the gap spacing. We show that this result is related to a modification of the interface boundary condition which incorporates stresses originated from normal velocity gradients. The early nonlinear regime shows nearly no competition between the outgrowing fingers, characteristic of low viscosity contrast flows. We perform experiments in a wide range of experimental parameters, under conditions of mass conservation (no injection), and characterize the resulting patterns by data collapses of two characteristic lengths: the radius of gyration of the pattern and the interface stretching. Deep in the nonlinear regime, the fingers which grow radially outwards stretch and become gradually thinner, to a point that the fingers pinch and emit drops. We show that the amount of liquid emitted in the first generation of drops is a constant independent of the experimental parameters. Further on there is a sharp reduction of the amount of liquid centrifugated, punctuated by periods of no observable centrifugation.

Expanding scroll rings and negative tension turbulence in a model of excitable media

Scroll waves in excitable media, described by the Barkley model, are studied. In the parameter region of weak excitability, negative tension of wave filaments is found. It leads to expansion of scroll rings and instability of wave filaments. A circular filament tends to stretch, bend, loop, and produce an expanding tangle that fills up the volume. The filament does not undergo fragmentation before it touches the boundaries. Statistical properties of such Winfree turbulence of scroll waves are numerically investigated.

Simulando una vía de comunicación: el tramo de la vía romana entre el Coll de Panissars y Girona

Mediante programa informático es posible simular las vías de comunicación existentes entre asentamientos humanos, siendo muchos los trabajos publicados y varias las formas de aproximación al problema. El objetivo del trabajo que nos ocupa ha consistido en la generación de un nuevo modelo a partir del cual fuera posible simular una vía de comunicación, con la salvedad de que en este caso ya existía una idea aproximada de cual sería su trayectoria a partir de trabajos de otros autores. Esta vía de comunicación es la vía Augusta, en el tramo que une el Coll de Panissars y la ciudad de Girona.

Effects of coastal submarine canyons on tsunami propagation and impact

We analyse the variations produced on tsunami propagation and impact over a straight coastline because of the presence of a submarine canyon incised in the continental margin. For ease of calculation we assume that the shoreline and the shelf edge are parallel and that the incident wave approaches them normally. A total of 512 synthetic scenarios have been computed by combining the bathymetry of a continental margin incised by a parameterised single canyon and the incident tsunami waves. The margin bathymetry, the canyon and the tsunami waves have been generated using mathematical functions (e.g. Gaussian). Canyon parameters analysed are: (i) incision length into the continental shelf, which for a constant shelf width relates directly to the distance from the canyon head to the coast, (ii) canyon width, and (iii) canyon orientation with respect to the shoreline. Tsunami wave parameters considered are period and sign. The COMCOT tsunami model from Cornell University was applied to propagate the waves across the synthetic bathymetric surfaces. Five simulations of tsunami propagation over a non-canyoned margin were also performed for reference. The analysis of the results reveals a strong variation of tsunami arrival times and amplitudes reaching the coastline when a tsunami wave travels over a submarine canyon, with changing maximum height location and alongshore extension. In general, the presence of a submarine canyon lowers the arrival time to the shoreline but prevents wave build-up just over the canyon axis. This leads to a decrease in tsunami amplitude at the coastal stretch located just shoreward of the canyon head, which results in a lower run-up in comparison with a non-canyoned margin. Contrarily, an increased wave build-up occurs on both sides of the canyon head, generating two coastal stretches with an enhanced run-up. These aggravated or reduced tsunami effects are modified with (i) proximity of the canyon tip to the coast, amplifying the wave height, (ii) canyon width, enlarging the areas with lower and higher maximum height wave along the coastline, and (iii) canyon obliquity with respect to the shoreline and shelf edge, increasing wave height shoreward of the leeward flank of the canyon. Moreover, the presence of a submarine canyon near the coast produces a variation of wave energy along the shore, eventually resulting in edge waves shoreward of the canyon head. Edge waves subsequently spread out alongshore reaching significant amplitudes especially when coupling with tsunami secondary waves occurs. Model results have been groundtruthed using the actual bathymetry of Blanes Canyon area in the North Catalan margin. This paper underlines the effects of the presence, morphology and orientation of submarine canyons as a determining factor on tsunami propagation and impact, which could prevail over other effects deriving from coastal configuration.

Usos del sòl i activitats productives a Barcelona a partir de l"anàlisi paleoambiental de la llacuna litoral medieval del Pa de Palau

[cat]Els treballs arqueològics duts a terme al solar localitzat entre la plaça de Pau Vila i els carrers del Dr. Aiguader i de la Marquesa, al peu del baluard del Migdia, han permès descriure l'evolució geoarqueològica d'un tram del front marítim de Barcelona i generar dades fiables de la configuració de la façana litoral. Sota una potent capa de sorres, s'identificà un paquet de llims i argiles orgàniques característiques d'un medi de baixa energia; és a dir, protegit de l'onatge marí probablement per barres sorrenques. Aquesta seqüència presenta una cronologia entre finals del segle IX i ca. 1440, moment en què es realitzaren els primers intents de construcció d'estructures portuàries. En aquest nivell llimoargilós s'han dut a terme analítiques paleoambientals consistents en l'estudi pol·línic, sedimentològic i geoquímic dels sediments que han posat en evidència els canvis ambientals del front marítim barceloní, del paisatge vegetal del pla, com també de les activitats agrícoles i productives urbanes en època medieval i inicis de l'edat moderna. La informació així obtinguda ha estat contrastada amb la informació històrica. [eng]The archaeological work conducted at the site located at the foot of the Baluard del Migdia bastion between Plaça Pau Vila and Carrer Dr. Aiguader and Carrer Marquesa has provided the opportunity to describe the geoarchaeological evolution of a stretch of the seafront of Barcelona and to generate reliable data on the configuration of the coastline. A layer of silt and organic clays typical of a low-energy environment was found below a thick layer of sands. In other words, this packet was protected from the swell of the sea, probably by sandbars. This sequence presents a chronology spanning from the late 9th century BC to around 1440, the time when the first attempts were made to build port structures. This layer of silt and clay has been subjected to palaeoenvironmental analyses consisting of the study of the pollen, sediment and the geochemistry of the sediments. The results of this analysis provide evidence of the environmental changes in the seafront of Barcelona and the landscape in the plain, as well as the urban agricultural and production activities in medieval times and the early modern age. The information obtained has been compared with historical information.

Myofascial pain associated to trigger points: A literature review. Part 2: Differential diagnosis and treatment

During the last decades the advance in knowledge of myofascial pain has been constant in the medical and dental community. However, although several aspects have been clarified in relation to its epidemiology, clinical characteristics and etiopathogenesis, many uncertainties remain. Many clinical conditions are included in the differential diagnosis of myofascial pain associated to trigger points. A good anamnesis and clinical exploration is thus required in order to ensure correct diagnosis and treatment. Among the numerous treatments used in application to trigger points, the spray-and-stretch technique and direct injection targeted to such trigger points have been found to be the most effective options. In chronic cases, psychosocial intervention is required, due to the high incidence of mood disorders and/or anxiety observed in these patients, who in turn present a poorer prognosis. This underscores the importance of early diagnosis and treatment.

Reinforcement versus Fluidization in Cytoskeletal Mechanoresponsiveness

Every adherent eukaryotic cell exerts appreciable traction forces upon its substrate. Moreover, every resident cell within the heart, great vessels, bladder, gut or lung routinely experiences large periodic stretches. As an acute response to such stretches the cytoskeleton can stiffen, increase traction forces and reinforce, as reported by some, or can soften and fluidize, as reported more recently by our laboratory, but in any given circumstance it remains unknown which response might prevail or why. Using a novel nanotechnology, we show here that in loading conditions expected in most physiological circumstances the localized reinforcement response fails to scale up to the level of homogeneous cell stretch; fluidization trumps reinforcement. Whereas the reinforcement response is known to be mediated by upstream mechanosensing and downstream signaling, results presented here show the fluidization response to be altogether novel: it is a direct physical effect of mechanical force acting upon a structural lattice that is soft and fragile. Cytoskeletal softness and fragility, we argue, is consistent with early evolutionary adaptations of the eukaryotic cell to material properties of a soft inert microenvironment.

Little evidence for association between the TGFBR1*6A variant and colorectal cancer: a family-based association study on non-syndromic family members from Australia and Spain.

Genome-wide linkage studies have identified the 9q22 chromosomal region as linked with colorectal cancer (CRC) predisposition. A candidate gene in this region is transforming growth factor beta receptor 1 (TGFBR1). Investigation of TGFBR1 has focused on the common genetic variant rs11466445, a short exonic deletion of nine base pairs which results in truncation of a stretch of nine alanine residues to six alanine residues in the gene product. While the six alanine (*6A) allele has been reported to be associated with increased risk of CRC in some population based study groups this association remains the subject of robust debate. To date, reports have been limited to population-based case-control association studies, or case-control studies of CRC families selecting one affected individual per family. No study has yet taken advantage of all the genetic information provided by multiplex CRC families. Methods: We have tested for an association between rs11466445 and risk of CRC using several family-based statistical tests in a new study group comprising members of non-syndromic high risk CRC families sourced from three familial cancer centres, two in Australia and one in Spain. Results: We report a finding of a nominally significant result using the pedigree-based association test approach (PBAT; p = 0.028), while other family-based tests were non-significant, but with a p-value < 0.10 in each instance. These other tests included the Generalised Disequilibrium Test (GDT; p = 0.085), parent of origin GDT Generalised Disequilibrium Test (GDT-PO; p = 0.081) and empirical Family-Based Association Test (FBAT; p = 0.096, additive model). Related-person case-control testing using the 'More Powerful' Quasi-Likelihood Score Test did not provide any evidence for association (M-QL5; p = 0.41). Conclusions: After conservatively taking into account considerations for multiple hypothesis testing, we find little evidence for an association between the TGFBR1*6A allele and CRC risk in these families. The weak support for an increase in risk in CRC predisposed families is in agreement with recent meta-analyses of case-control studies, which estimate only a modest increase in sporadic CRC risk among 6*A allele carriers.

Structure and dynamics of the membrane attaching nitric oxide transporter nitrophorin 7

Nitrophorins represent a unique class of heme proteins that are able to perform the delicate transportation and release of the free-radical gaseous messenger nitric oxide (NO) in a pH-triggered manner. Besides its ability to bind to phospholipid membranes, the N-terminus contains an additional Leu-Pro-Gly stretch, which is a unique sequence trait, and the heme cavity is significantly altered with respect to other nitrophorins. These distinctive features encouraged us to solve the X-ray crystallographic structures of NP7 at low and high pH and bound with different heme ligands (nitric oxide, histamine, imidazole). The overall fold of the lipocalin motif is well preserved in the different X-ray structures and resembles the fold of other nitrophorins. However, a chain-like arrangement in the crystal lattice due to a number of head-to-tail electrostatic stabilizing interactions is found in NP7. Furthermore, the X-ray structures also reveal ligand-dependent changes in the orientation of the heme, as well as in specific interactions between the A-B and G-H loops, which are considered to be relevant for the biological function of nitrophorins. Fast and ultrafast laser triggered ligand rebinding experiments demonstrate the pH-dependent ligand migration within the cavities and the exit route. Finally, the topological distribution of pockets located around the heme as well as from inner cavities present at the rear of the protein provides a distinctive feature in NP7, so that while a loop gated exit mechanism to the solvent has been proposed for most nitrophorins, a more complex mechanism that involves several interconnected gas hosting cavities is proposed for NP7.