Whole genome analysis of p38 SAPK-mediated gene expression upon stress

Background: Cells have the ability to respond and adapt to environmental changes through activation of stress-activated protein kinases (SAPKs). Although p38 SAPK signalling is known to participate in the regulation of gene expression little is known on the molecular mechanisms used by this SAPK to regulate stress-responsive genes and the overall set of genes regulated by p38 in response to different stimuli.Results: Here, we report a whole genome expression analyses on mouse embryonic fibroblasts (MEFs) treated with three different p38 SAPK activating-stimuli, namely osmostress, the cytokine TNFα and the protein synthesis inhibitor anisomycin. We have found that the activation kinetics of p38α SAPK in response to these insults is different and also leads to a complex gene pattern response specific for a given stress with a restricted set of overlapping genes. In addition, we have analysed the contribution of p38α the major p38 family member present in MEFs, to the overall stress-induced transcriptional response by using both a chemical inhibitor (SB203580) and p38α deficient (p38α-/-) MEFs. We show here that p38 SAPK dependency ranged between 60% and 88% depending on the treatments and that there is a very good overlap between the inhibitor treatment and the ko cells. Furthermore, we have found that the dependency of SAPK varies depending on the time the cells are subjected to osmostress. Conclusions: Our genome-wide transcriptional analyses shows a selective response to specific stimuli and a restricted common response of up to 20% of the stress up-regulated early genes that involves an important set of transcription factors, which might be critical for either cell adaptation or preparation for continuous extra-cellular changes. Interestingly, up to 85% of the up-regulated genes are under the transcriptional control of p38 SAPK. Thus, activation of p38 SAPK is critical to elicit the early gene expression program required for cell adaptation to stress.

Aspectos socioculturales de la precariedad laboral y de su impacto

This essay analyzes the current work crisis, its individual and social influence and its implications on human development and health. It discusses the main cultural perspectives on the notion of work and presents the hypothesis of a possible influence of the cultural meaning of the concept of 'work' on diminishing self-esteem and mental health problems among unemployed people. The essay also highlights possible implications of the current situation in the future taking into consideration socioeconomic and political aspects as well as global demographic growth. Finally, the essay discusses the role that Public Health should have not only in the prevention and control of health problems among the unemployed, but also in the social, economic and cultural changes that are considered necessary to mitígate the serious social differences and to improve the current situation.

Aspectos socioculturales de la precariedad laboral y de su impacto

This essay analyzes the current work crisis, its individual and social influence and its implications on human development and health. It discusses the main cultural perspectives on the notion of work and presents the hypothesis of a possible influence of the cultural meaning of the concept of 'work' on diminishing self-esteem and mental health problems among unemployed people. The essay also highlights possible implications of the current situation in the future taking into consideration socioeconomic and political aspects as well as global demographic growth. Finally, the essay discusses the role that Public Health should have not only in the prevention and control of health problems among the unemployed, but also in the social, economic and cultural changes that are considered necessary to mitígate the serious social differences and to improve the current situation.

Verifique sus conocimientos sobre aspectos generales de fisiopatología

Para proporcionar cuidados de calidad a los pacientes es imprescindible tener unos conocimientos esenciales de fisiopatología, que es la ciencia que estudia los trastornos que se producen en las estructuras y en la función de los órganos y que comportan el proceso de enfermar. Ya que en ocasiones es difícil establecer una diferencia clara entre las causas y los procesos que ocasionan una enfermedad, la fisiopatología también estudia dichas causas. El término patogenia define más específicamente los mecanismos por los que los agentes causales ocasionan las lesiones, mientras que la denominación de fisiopatología se usa para el estudio del curso normal de los procesos que explican la aparición y el desarrollo...