12 resultados para Nós Críticos

em Consorci de Serveis Universitaris de Catalunya (CSUC), Spain


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Aquest projecte es basa en l'estudi de l'oferiment de qualitat de servei en xarxes wireless i satel·litals. Per això l'estudi de les tècniques de cross-layer i del IEEE 802.11e ha sigut el punt clau per al desenvolupament teòric d’aquest estudi. Usant el simulador de xarxes network simulator, a la part de simulacions es plantegen tres situacions: l'estudi de la xarxa satel·lital, l'estudi del mètode d'accés HCCA i la interconnexió de la xarxa satel·lital amb la wireless. Encara que aquest últim punt, incomplet en aquest projecte, ha de ser la continuació per a futures investigacions.

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En aquest projecte es presenta un escenari de xarxa DTN. Aquest escenari té una serie de problemes que dificulten l’encaminament. Al projecte s’ha proposat un protocol d’encaminament que permet superar aquestes dificultats. Per realitzar això ha estat necessari estudiar i adquirir un coneixement profund sobre el funcionament dels protocols de xarxes DTN. Per demostrar que aquesta proposta solucionava els problemes es presenta l’anàlisi dels resultats d’aplicar el protocol a l’escenari, aquests resultats s’han obtingut amb l’eina de simulació de xarxes NS-2.

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El deliri és un trastorn neuropsiquiàtric que poden patir els pacients greus a les unitats de cures intensives. La seva aparició s’associa amb un augment de la morbimortalitat. La seva incidència varia entre el 20-80% segons els mètodes diagnòstics i la situació clínica del pacient. L’objectiu principal del estudi fou avaluar la incidència de delirium en la Unitat de Reanimació de l’Hospital Universitari Germans Trias i Pujol mitjançant el test ICDSC i associar el delirium amb la edat, severitat del pacient, urgència de la cirurgia, especialitat quirúrgica i opinió del equip d‘infermeria sobre el test. Durant 3 mesos es van incloure 50 pacients que van ser avaluats amb el ICDSC dues vegades al dia, durant 5 dies. La incidència de delirium a la unitat fou del 18%. Es va trobar una associació estadísticament significativa entre delirium i la edat i el APACHE II score al ingrés. És recomanable monitoritzar diàriament el delirium i tractar-lo com a un signe vital més. El test ICDSC és un test senzill i fàcil que ens ajuda a no infravalorar el delirium del pacient crític.

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Objectiu: Avaluar una intervenció dirigida a la reducció d‘infeccions relacionades amb els catèters venosos centrals (IRC). Mètodes: Estudi pre-postintervenció, 2004-2006. Població d'estudi: pacients portadors de catèter venós central (CVC). La intervenció va consistir en conèixer la situació bassal, identificar factors de risc, i en el segon període realitzar diverses intervencions. Resultats: Es van analitzar 175 i 200 CVC en el període pre i postintervenció, respectivament. Es va observar una incidència de IRC de 20% durant el període preintervenció i de 5,5% en el període post-intervenció (p&0,001). Conclusió: La aplicació de mesures de prevenció ha aconseguit una disminució d’aquestes del 72,5%.

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This report is the document summarizing the research project as final work research project done as a final work of the degree in Computer Engineering to determine which are the critical success factors in the implementation of a business strategy process management (BPM).

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El objetivo principal del presente trabajo pretende demostrar el potencial del software libre frente al software propietario que actualmente utiliza la empresa Systemum.

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El presente trabajo consiste en la traducción del alemán al español de un fragmento del libro NS-Offizzier war ich nicht (Ute Althaus), en el cual se aborda el tópico del nacionalsocialismo desde el ámbito familiar y bajo una perspectiva psico-social.

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Proteins of the Hha/YmoA family co-regulate with H-NS the expression of horizontally acquired genes in Enterobacteria. Systematic mutations of conserved acidic residues in Hha have allowed the identification of D48 as an essential residue for H-NS binding and the involvement of E25. Mutations of these residues resulted in deregulation of sensitive genes in vivo. D48 is only partially solvent accessible, yet it defines the functional binding interface between Hha and H-NS confirming that Hha has to undergo a conformational change to bind H-NS. Exposed acidic residues, such as E25, may electrostatically facilitate and direct the approach of Hha to the positively charged region of H-NS enabling the formation of the final complex when D48 becomes accessible by a conformational change of Hha.

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The nucleoid-associated protein H-NS is a global modulator of the expression of genes associated with adaptation to environmental changes. A variant of H-NS expressed in the R27 plasmid was previously shown to selectively modulate the expression of horizontally acquired genes, with minimal effects on core genes that are repressed by the chromosomal form of H-NS. Both H-NS proteins are formed by an oligomerization domain and a DNA-binding domain, which are connected by a linker that is highly flexible in the absence of DNA. We studied DNA binding by means of oligomer-forming chimeric proteins in which domains of the chromosomal and plasmidic variants are exchanged, as well as in monomeric truncated forms containing the DNA-binding domain and variable portions of the linker. Point mutations in the linker were also examined in full-length and truncated H-NS constructs. These experiments show that the linker region contributes to DNA binding affinity and that it is a main component of the distinct DNA binding properties of chromosomal and plasmidic H-NS. We propose that interactions between the linker and DNA limit the flexibility of the connection between H- NS oligomerization and DNA binding and provide an allosteric indirect readout mechanism to detect long- range distortions of DNA, thus enabling discrimination between core and horizontally acquired DNA.

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Horizontal acquisition of DNA by bacteria dramatically increases genetic diversity and hence successful bacterial colonization of several niches, including the human host. A relevant issue is how this newly acquired DNA interacts and integrates in the regulatory networks of the bacterial cell. The global modulator H-NS targets both core genome and HGT genes and silences gene expression in response to external stimuli such as osmolarity and temperature. Here we provide evidence that H-NS discriminates and differentially modulates core and HGT DNA. As an example of this, plasmid R27-encoded H-NS protein has evolved to selectively silence HGT genes and does not interfere with core genome regulation. In turn, differential regulation of both gene lineages by resident chromosomal H-NS requires a helper protein: the Hha protein. Tight silencing of HGT DNA is accomplished by H-NS-Hha complexes. In contrast, core genes are modulated by H-NS homoligomers. Remarkably, the presence of Hha-like proteins is restricted to the Enterobacteriaceae. In addition, conjugative plasmids encoding H-NS variants have hitherto been isolated only from members of the family. Thus, the H-NS system in enteric bacteria presents unique evolutionary features. The capacity to selectively discriminate between core and HGT DNA may help to maintain horizontally transmitted DNA in silent form and may give these bacteria a competitive advantage in adapting to new environments, including host colonization.

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Horizontal acquisition of DNA by bacteria dramatically increases genetic diversity and hence successful bacterial colonization of several niches, including the human host. A relevant issue is how this newly acquired DNA interacts and integrates in the regulatory networks of the bacterial cell. The global modulator H-NS targets both core genome and HGT genes and silences gene expression in response to external stimuli such as osmolarity and temperature. Here we provide evidence that H-NS discriminates and differentially modulates core and HGT DNA. As an example of this, plasmid R27-encoded H-NS protein has evolved to selectively silence HGT genes and does not interfere with core genome regulation. In turn, differential regulation of both gene lineages by resident chromosomal H-NS requires a helper protein: the Hha protein. Tight silencing of HGT DNA is accomplished by H-NS-Hha complexes. In contrast, core genes are modulated by H-NS homoligomers. Remarkably, the presence of Hha-like proteins is restricted to the Enterobacteriaceae. In addition, conjugative plasmids encoding H-NS variants have hitherto been isolated only from members of the family. Thus, the H-NS system in enteric bacteria presents unique evolutionary features. The capacity to selectively discriminate between core and HGT DNA may help to maintain horizontally transmitted DNA in silent form and may give these bacteria a competitive advantage in adapting to new environments, including host colonization.

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[spa]Espacios Críticos" es el nombre de una colección editorial y de un proyecto intelectual cuyo objetivo es analizar, contextualizar y traducir algunos textos fundamentales de pensadores espaciales críticos actuales. Se presentan el alcance, los objetivos y los resultados de dicho proyecto, resumiendo las aportaciones de los primeros volúmenes ya publicados.