Planta de producción de ácido adípico

El objetivo de este proyecto es el diseño y estudio de una planta en continuo para la producción de 60000 Tn/año de ácido adípico a partir de la oxidación del ciclohexanol con ácido nítrico. Debe incluir también la construcción, el diseño de los equipos, el montaje, la puesta en marcha de la planta y la operación de ésta en estado estacionario.

Integración de empresas: planificación y gestión del cambio

Este proyecto nace de la necesidad de realizar una integración de los sistemas informáticos de una empresa en una multinacional; las empresas disponen de servicios de outsourcing en sistemas de información. Al tratarse de una integración operativa, sólo trataremos de trasladar los servicios de centro de atención al usuario, soporte local y ofimática desde un outsourcing de servicios a otro, añadiendo los cambios de procesos necesarios para disponer de un único entorno de trabajo. El cambio de entorno de trabajo se ha de realizar sin perdida de disponibilidad ni de información.

La utilització dels sistemes de control electrònic monitorat a la població penada de Catalunya

La present recerca té per objecte l’estudi de la utilització dels sistemes de control electrònic monitorat a la població penada a Catalunya. La finalitat principal del projecte és la d’analitzar l’adequació d’aquests sistemes als fins que la llei penal, processal i penitenciària els encomana i valorar la viabilitat d’ampliació de la utilització d’aquests sistemes. L’interès en la realització d’aquest estudi deriva de la constatació, per una banda, de l’existència d’una àmplia oferta de sistemes tecnològics, en constant desenvolupament i millora, que permeten un seguiment i control dels individus en la comunitat, i per altra banda, de la progressiva incorporació d’aquestes tecnologies en la legislació penal i penitenciària. La recerca parteix de la base que en l’actual context de presons superpoblades, de creixent volum de la població interna en centres penitenciaris i de recerca de mesures de major control del risc, l’anàlisi de l’ús que s’ha donat a aquest recurs legal i la valoració que de la seva utilització en fan els subjectes involucrats en la seva aplicació pot resultar cabdal per al disseny de noves polítiques penitenciàries. A més, la recerca hauria de permetre plantejar l’eventual extensió d’aquests sistemes a un major nombre de supòsits en el mateix context del tercer grau penitenciari o bé fins i tot plantejar la seva utilització en la llibertat condicional o en altres àmbits de l’execució penal.

Framework para la capa de presentación de aplicaciones web

El framework dissenyat, denominat magis, ajuda als desenvolupadors organitzant la presentació de continguts, l'accés a les funcions i serveis de negoci, el flux de pàgines, el processament de formularis, el maneig d'errors i la gestió d'estat.

Desarrollo de aplicaciones para dispositivos móviles : copy2Cloud

Copy2Cloud, que es como he denominado en un principio mi aplicación, básicamente realizará varias copias de elementos indispensables de nuestro dispositivo móvil en un servicio de ficheros tipo nube. Como bien sabemos el espacio físico de los dispositivos es cada vez más limitado, las aplicaciones y datos cada vez ocupan más, y esto nos lleva a tener descontrol sobre el almacenaje y veracidad de éstos, es decir, tenemos tal cantidad de fotos y videos personales en nuestros terminales que en caso de desastre la mayoría de las personas sin conocimientos técnicos darían por perdida toda esa acumulación de nuestros recuerdos, de parte de nuestras vidas.

Propuesta creativa para la nueva línea de vinos ENJOYME

El projecte presentat en aquest document formal conté la proposta per a la nova marca de vins Enjoyme, el projecte consta de la creació de diverses peces gràfiques per tal de construir una proposta global per a la marca. L’encàrrec per part del client consta de: construcció de marca Enjoyme (logotip), lloc web enfocat a la venda on line, packaging del producte, OPI i revista digital. Cadascuna d’aquestes peces es troba explicada detalladament dins d’aquest document amb les seves respectives especificacions creatives, tècniques, estratègiques i econòmiques. El concepte creatiu d’aquesta campanya “Cultiva els teus sentits” està reflectit en cadascuna de les peces, formant un equilibri en conjunt. La idea central de la campanya s’enfoca a transmetre als clients totes les sensacions que li pot donar la natura en ser humà, tenint en compte l’orgànic i biodinàmic com a part fonamental del projecte, ja que aquest producte elaborat amb aquest tipus d’agricultura.

Counter-ion effect on surfactant-DNA gel particles as controlled DNA delivery systems

The ability to entrap drugs within vehicles and subsequently release them has led to new treatments for a number of diseases. Based on an associative phase separation and interfacial diffusion approach, we developed a way to prepare DNA gel particles without adding any kind of cross-linker or organic solvent. Among the various agents studied, cationic surfactants offered particularly efficient control for encapsulation and DNA release from these DNA gel particles. The driving force for this strong association is the electrostatic interaction between the two components, as induced by the entropic increase due to the release of the respective counter-ions. However, little is known about the influence of the respective counter-ions on this surfactant-DNA interaction. Here we examined the effect of different counter-ions on the formation and properties of the DNA gel particles by mixing DNA (either single- (ssDNA) or double-stranded (dsDNA)) with the single chain surfactant dodecyltrimethylammonium (DTA). In particular, we used as counter-ions of this surfactant the hydrogen sulfate and trifluoromethane sulfonate anions and the two halides, chloride and bromide. Effects on the morphology of the particles obtained, the encapsulation of DNA and its release, as well as the haemocompatibility of these particles, are presented, using the counter-ion structure and the DNA conformation as controlling parameters. Analysis of the data indicates that the degree of counter-ion dissociation from the surfactant micelles and the polar/hydrophobic character of the counter-ion are important parameters in the final properties of the particles. The stronger interaction with amphiphiles for ssDNA than for dsDNA suggests the important role of hydrophobic interactions in DNA.

Membrane-destabilizing activity of pH-responsive cationic lysine-based surfactants: role of charge position and alkyl chain length

Many strategies for treating diseases require the delivery of drugs into the cell cytoplasm following internalization within endosomal vesicles. Thus, compounds triggered by low pH to disrupt membranes and release endosomal contents into the cytosol are of particular interest. Here, we report novel cationic lysine-based surfactants (hydrochloride salts of N¿- and N¿-acyl lysine methyl ester) that differ in the position of the positive charge and the length of the alkyl chain. Amino acid-based surfactants could be promising novel biomaterials in drug delivery systems, given their biocompatible properties and low cytotoxic potential. We examined their ability to disrupt the cell membrane in a range of pH values, concentrations and incubation times, using a standard hemolysis assay as a model of endosomal membranes. Furthermore, we addressed the mechanism of surfactant-mediated membrane destabilization, including the effects of each surfactant on erythrocyte morphology as a function of pH. We found that only surfactants with the positive charge on the ¿-amino group of lysine showed pH-sensitive hemolytic activity and improved kinetics within the endosomal pH range, indicating that the positive charge position is critical for pH-responsive behavior. Moreover, our results showed that an increase in the alkyl chain length from 14 to 16 carbon atoms was associated with a lower ability to disrupt cell membranes. Knowledge on modulating surfactant-lipid bilayer interactions may help us to develop more efficient biocompatible amino acid-based drug delivery devices.

dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes

H3K4me3 is a histone modification that accumulates at the transcription-start site (TSS) of active genes and is known to be important for transcription activation. The way in which H3K4me3 is regulated at TSS and the actual molecular basis of its contribution to transcription remain largely unanswered. To address these questions, we have analyzed the contribution of dKDM5/LID, the main H3K4me3 demethylase in Drosophila, to the regulation of the pattern of H3K4me3. ChIP-seq results show that, at developmental genes, dKDM5/LID localizes at TSS and regulates H3K4me3. dKDM5/LID target genes are highly transcribed and enriched in active RNApol II and H3K36me3, suggesting a positive contribution to transcription. Expression-profiling show that, though weakly, dKDM5/LID target genes are significantly downregulated upon dKDM5/LID depletion. Furthermore, dKDM5/LID depletion results in decreased RNApol II occupancy, particularly by the promoter-proximal Pol lloser5 form. Our results also show that ASH2, an evolutionarily conserved factor that locates at TSS and is required for H3K4me3, binds and positively regulates dKDM5/LID target genes. However, dKDM5/LID and ASH2 do not bind simultaneously and recognize different chromatin states, enriched in H3K4me3 and not, respectively. These results indicate that, at developmental genes, dKDM5/LID and ASH2 coordinately regulate H3K4me3 at TSS and that this dynamic regulation contributes to transcription.

Pure sensory neuropathy in patients with primary Sjogren's syndrome: clinical, immunological and electromyographic findings

A pure sensory neuropathy caused by lymphocytic infiltration of the dorsal root ganglia has been reported in a few patients with Sjögren's syndrome. The clinical, immunological, and electromyographic findings of five patients with this type of neuropathy and primary Sjögren's syndrome were reviewed. Typical clinical indications were the presence of a chronic asymmetrical sensory deficit, initial disease in the hands with a predominant loss of the vibratory and joint position senses, and an association with Adie's pupil syndrome or trigeminal sensory neuropathy. The simultaneous impairment of the central and peripheral evoked cortical potentials suggested that there was a lesion of the neuronal cell body. The neuropathy preceded the diagnosis of Sjögren's syndrome in four patients. Four patients were positive for Ro antibodies, but systemic vasculitis or malignancy was not found after a mean follow up of six years. These findings indicate that in patients with a sensory neuropathy the diagnosis of Sjögren's syndrome has to be considered, even if the patient denies the presence of sicca symptoms, and that appropriate tests must be carried out.

Splenectomy for refractory Evan' syndrome associated with antiphospholipid antibodies: report of two cases.

The main haematological manifestations seen in patients with antiphospholipid antibodies (aPL) are thrombocytopenia, usually mild, and haemolytic anaemia with a positive Coombs test. Owing to the shared characteristics with idiopathic thrombocytopenic purpura, similar rules are followed in the treatment of these cytopenias. Two patients with severe aPL associated cytopenias, who required splenectomy after being refractory to steroids, immunosuppressive agents, and other treatments (intravenous gammaglobulin, danazol), are described, and previously reported cases are reviewed.