Cell-Cell interaction in erythropoiesis: role of human monocytes

Erythroid burst forming units (BFU-E) are proliferative cells present in peripheral blood and bone marrow which may be precursors of the erythroid colony forming cell found in the bone marrow. To examine the possible role of monocyte-macrophages in the modulation of erythropoiesis, the effect of monocytes on peripheral blood BFU-E proliferation in response to erythropoietin was investigated in the plasma clot culture system. Peripheral blood mononuclear cells from normal human donors were separated into four fractions. Fraction-I cells were obtained from the interface of Ficoll-Hypaque gradients (20-30% monocytes; 60-80% lymphocytes); fraction-II cells were fraction-I cells that were nonadherent to plastic (2-10% monocytes; 90-98% lymphocytes); fraction-III cells were obtained by incubation of fraction-II cells with carbonyl iron followed by Ficoll-Hypaque centrifugation (>99% lymphocytes); and fraction-IV cells represented the adherent population of fraction-II cells released from the plastic by lidocaine (>95% monocytes). When cells from these fractions were cultured in the presence of erythropoietin, the number of BFU-E-derived colonies was inversely proportional to the number of monocytes present (r = ¿0.96, P < 0.001). The suppressive effect of monocytes on BFU-E proliferation was confirmed by admixing autologous purified monocytes (fraction-IV cells) with fraction-III cells. Monocyte concentrations of ¿20% completely suppressed BFU-E activity. Reduction in the number of plated BFU-E by monocyte dilution could not account for these findings: a 15% reduction in the number of fraction-III cells plated resulted in only a 15% reduction in colony formation. These results indicate that monocyte-macrophages may play a significant role in the regulation of erythropoiesis and be involved in the pathogenesis of the hypoproliferative anemias associated with infection and certain neoplasia in which increased monocyte activity and monopoiesis also occur.

Feeding ecology and movements of the Barolo shearwater Puffinus baroli baroli in the Azores, NE Atlantic

Trophic ecology and movements are critical issues for understanding the role of marine predators in food webs and for facing the challenges of their conservation. Seabird foraging ecology has been increasingly studied, but small elusive species, such as those forming the"little shearwater" complex, remain poorly known. We present the first study on the movements and feeding ecology of the Barolo shearwater Puffinus baroli baroli in a colony from the Azores archipelago (NE Atlantic), combining global location-sensing units, stable isotope analyses of feathers (δ13C and δ15N), stomach flushings and data from maximum depth gauges. During the chick-rearing period, parents visited their nests most nights, foraged mainly south of the colony and fed at lower trophic levels than during the non-breeding period. Squid was the most diverse prey (6 families and at least 10 different taxa), but species composition varied considerably between years. Two squid families, Onychoteuthidae and Argonautidae, and the fish family Phycidae accounted for 82.3% of ingested prey by number. On average, maximum dive depths per foraging trip reached 14.8 m (range: 7.9 to 23.1 m). After the breeding period, birds dispersed offshore in all directions and up to 2500 km from the breeding colony, and fed at higher trophic levels. Overall, our results indicate that the Barolo shearwater is a non-migratory shearwater feeding at the lowest trophic level among Macaronesian seabirds, showing both diurnal and nocturnal activity and feeding deeper in the water column, principally on small schooling squid and fish. These traits contrast with those of 3 other Azorean Procellariiformes (Cory"s shearwater Calonectris diomedea, the Madeiran storm-petrel Oceanodroma castro and Monteiro"s storm-petrel O. monteiroi), indicating ecological segregation within the Azorean seabird community.

Forming efficient networks

In this paper, we suggest a simple sequential mechanism whose subgame perfect equilibria give rise to efficient networks. Moreover, the payoffs received by the agents coincide with their Shapley value in an appropriately defined cooperative game.

Desenvolupament de mesures per comparar transculturalment xarxes personals (USA i Estats Units)

Estudi elaborat a partir d’una estada a la Universitat de Florida durant Abril i Maig de 2006. “Application to Immigrant Populations in South Florida and Northeastern Spain" ha permès recollir les xarxes personals d’immigrants en els dos països mitjançant un qüestionari implementat amb un software desenvolupat ex professo per recollir i presentar visualment les dades. Es van revisar i assemblar les entrevistes i qüestionaris realitzats a Catalunya i a Estats Units (Miami i Nova York, especialment). Fins al moment s’han revisat i analitzat uns 450 casos. Un cop depurada i analitzada la informació obtinguda s’ha pogut disposar per primer cop de mesures globals pels diferents col•lectius estudiats. L'objectiu global del projecte és entendre les implicacions que les estructures de les xarxes personals tenen en relació a un conjunt de conductes (de salut, d'ús de la llengua, etx) i les autoconcepcions. En aquest sentit era necessari desenvolupar un seguit de mesures que permetessin comparar i documentar la variació de les estructures de les xarxes personals a diferents cultures, nivells socioeconòmics, gènere, religió, etc. i incorporar-les com a variables independents als models explicatius.En aquest moments s’està desenvolupant un índex basat en variables estructurals (número de components de la xarxa, densitat, grau d’intermediació, etc.) i variables de composició (proporció de persones diferents del país d’origen, entre d'altres). La idea és disposar d’un índex d’heterogeneïtat de la xarxa social comparable entre els diferents col•lectius. Malgrat que el treball continua, la principal conclusió a la que s’ha arribat és que al menys a Espanya a mida que passa el temps augmenta el nivell d’heterogeneïtat de les xarxes personals. És a dir, que desprès d’un ràpid procés de canvi i una fase de transnacionalitat la tendència és a reduir aquest nivell (pels costos socials i econòmics que comporta) depenent, naturalment, de factors com l’origen temporal (primera onada o successives), el tipus de col•lectiu i el sexe.

Cosa: arqueologia de un enclave comercial mediterráneo. Campaña de 2006

En el marco del proyecto “La ciutat romana de Cosa: arqueologia d’un enclau comercial mediterrani” , autorizado y apoyado por la Soprintendenza Archeologica por la Toscana, entre los dias 4 y 22 de septiembre de 2006 se ha realizado la segunda campaña de intervenciones arqueológicas en la ciudad romana de Cosa (Ansedonia, prov. Grosseto, Itàlia), colonia latina fundada en el 273 aC a unos 120 km. al norte de Roma. De acuerdo a los resultados obtenidos en la campanya del 2005 (localización/verificación de los límites precisos de la ínsula O-P/4-5 mediante la aplicación de técnicas de prospección geofísica completadas con la limpieza, registro y documentación arqueológica de las estructuras localizadas ) los trabajos del 2006 se han orientado hacia la identificación de la organización interna de la dicha ínsula tomando como referente el criptopórtico situado en el extremo N.E., el cual parece constituir el límite de una estructura singular (privada o pública ) estratégicamente ubicada en relación al fórum i a la Via Sacra. El trabajo de campo ha consistido en un intenso decapage con la finalidad de delimitar unidades de habitación complejas funcionalmente definidas y así la articulación existente entre ellas; en este sentido se ha podido documentar evidencias del espacio porticado superpuesto al criptopórtico así como, paralelamente a la calle 5 y en dirección a la Via Sacra, parte de habitaciones algunas de las cuales conservaban restos del pavimiento original, en un caso de mosaico. Paralelamente, se ha realizado el análisis en laboratorio de los materiales recuperados los cuales, aún procediendo de nivel superficial, empiezan a proporcionar datos sobre los diferentes momentos de ocupación de la zona, básicamente tardorepublicanos y augustales.

Desenvolupament de dipòsits institucionals als Estats Units a partir de l'inici del 2005

Aquest article analitza la situació dels dipòsits institucionals als EUA mitjançant una enquesta realitzada per la CNI a les seves institucions acadèmiques. Amb aquesta enquesta s'obtingué informació sobre el grau d'implementació dels dipòsits i la seva extensió, així com del tipus de materials. A més, va servir per conèixer l'opinió de les institucions sobre els dipòsits, i va quedar palès que la major part de les que no en posseeixen, tenen previst posar-ne un en funcionament, tot i que els preocupi el cost de manteniment. L'article també tracta la responsabilitat administrativa dels dipòsits, les polítiques que els gestionen, la possibilitat de compartir dipòsits entre institucions i la procedència dels materials que contenen.

Solid-phase synthesis and NMR studies of modified oligonucleotides forming triplex helix and of oligonucleopeptides mimicking the topoisomerase I-DNA covalent complex

Report for the scientific sojourn carried out at the Institut de Biologia Molecular de Barcelona of the CSIC –state agency – from april until september 2007. Topoisomerase I is an essential nuclear enzyme that modulates the topological status of DNA, facilitating DNA helix unwinding during replication and transcription. We have prepared the oligonucleotide-peptide conjugate Ac-NLeu-Asn-Tyr(p-3’TTCAGAAGC5’)-LeuC-CONH-(CH2)6-OH as model compound for NMR studies of the Topoisomerase I- DNA complex. Special attention was made on the synthetic aspects for the preparation of this challenging compound especially solid supports and protecting groups. The desired peptide was obtained although we did not achieve the amount of the conjugate needed for NMR studies. Most probably the low yield is due to the intrinsic sensitive to hydrolysis of the phosphate bond between oligonucleotide and tyrosine. We have started the synthesis and the structural characterization of oligonucleotides carrying intercalating compounds. At the present state we have obtained model duplex and quadruplex sequences modified with acridine and NMR studies are underway. In addition to this project we have successfully resolved the structure of a fusion peptide derived from hepatitis C virus envelope synthesized by the group of Dr. Haro and we have synthesized and started the characterization of a modified G-quadruplex.

Behavior study of pipes after forming, coating and bending

A study of the main types of coatings and its processes that modern industry commonly apply to prevent to the corrosion due to the environmental effects to energetic market pipelines have been done. Extracting main time and temperature range values, coating heat treatment recreation have been applied to x65 pipelines steel grade samples obtained from a pipe which was formed using UOE forming process. Experimental tensile tests and Charpy V‐Notch Impact test have been carried out for a deeply knowledge of the influence on the steel once this recreations are applied. The Yield Strength and toughness have been improved despite lower values in rupture strain and ductile‐brittle temperature transition have been obtained. Finite Element Method have been applied to simulate the entirely pipe cold bending process to predict the mechanical properties and behaviour of the pipe made from x65 steel grade under different conditions.

Uranium reserve, nuclear fuel cycle delusion, CO2 emission from the sea, and electricity supply: reflections after the fuel meltdown of Fukushima nuclear power units

The Great Tohoku-Kanto earthquake and resulting tsunami has brought considerable attention to the issue of the construction of new power plants. We argue in this paper, nuclear power is not a sustainable solution to energy problems. First, we explore the stock of uranium-235 and the different schemes developed by the nuclear power industry to exploit this resource. Second, we show that these methods, fast breeder and MOX fuel reactors, are not feasible. Third, we show that the argument that nuclear energy can be used to reduce CO2 emissions is false: the emissions from the increased water evaporation from nuclear power generation must be accounted for. In the case of Japan, water from nuclear power plants is drained into the surrounding sea, raising the water temperature which has an adverse affect on the immediate ecosystem, as well as increasing CO2 emissions from increased water evaporation from the sea. Next, a short exercise is used to show that nuclear power is not even needed to meet consumer demand in Japan. Such an exercise should be performed for any country considering the construction of additional nuclear power plants. Lastly, the paper is concluded with a discussion of the implications of our findings.