186 resultados para Joaquín Sorolla
A Survey on Detection Techniques to Prevent Cross-Site Scripting Attacks on Current Web Applications
Resumo:
A common operation in wireless ad hoc networks is the flooding of broadcast messages to establish network topologies and routing tables. The flooding of broadcast messages is, however, a resource consuming process. It might require the retransmission of messages by most network nodes. It is, therefore, very important to optimize this operation. In this paper, we first analyze the multipoint relaying (MPR) flooding mechanism used by the Optimized Link State Routing (OLSR) protocol to distribute topology control (TC) messages among all the system nodes. We then propose a new flooding method, based on the fusion of two key concepts: distance-enabled multipoint relaying and connected dominating set (CDS) flooding. We present experimental simulationsthat show that our approach improves the performance of previous existing proposals.
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Peer reviewed
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Inhibition of cholesterol export from late endosomes causes cellular cholesterol imbalance, including cholesterol depletion in the trans-Golgi network (TGN). Here, using Chinese hamster ovary (CHO) Niemann-Pick type C1 (NPC1) mutant cell lines and human NPC1 mutant fibroblasts, we show that altered cholesterol levels at the TGN/endosome boundaries trigger Syntaxin 6 (Stx6) accumulation into VAMP3, transferrin, and Rab11-positive recycling endosomes (REs). This increases Stx6/VAMP3 interaction and interferes with the recycling of αVβ3 and α5β1 integrins and cell migration, possibly in a Stx6-dependent manner. In NPC1 mutant cells, restoration of cholesterol levels in the TGN, but not inhibition of VAMP3, restores the steady-state localization of Stx6 in the TGN. Furthermore, elevation of RE cholesterol is associated with increased amounts of Stx6 in RE. Hence, the fine-tuning of cholesterol levels at the TGN-RE boundaries together with a subset of cholesterol-sensitive SNARE proteins may play a regulatory role in cell migration and invasion.
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Control of lipid droplet (LD) nucleation and copy number are critical, yet poorly understood, processes. We use model peptides that shift from the endoplasmic reticulum (ER) to LDs in response to fatty acids to characterize the initial steps of LD formation occurring in lipid-starved cells. Initially, arriving lipids are rapidly packed in LDs that are resistant to starvation (pre-LDs). Pre-LDs are restricted ER microdomains with a stable core of neutral lipids. Subsequently, a first round of"emerging" LDs is nucleated, providing additional lipid storage capacity. Finally, in proportion to lipid concentration, new rounds of LDs progressively assemble. Confocal microscopy and electron tomography suggest that emerging LDs are nucleated in a limited number of ER microdomains after a synchronized stepwise process of protein gathering, lipid packaging, and recognition by Plin3 and Plin2. A comparative analysis demonstrates that the acyl-CoA synthetase 3 is recruited early to the assembly sites, where it is required for efficient LD nucleation and lipid storage.
Resumo:
Control of lipid droplet (LD) nucleation and copy number are critical, yet poorly understood, processes. We use model peptides that shift from the endoplasmic reticulum (ER) to LDs in response to fatty acids to characterize the initial steps of LD formation occurring in lipid-starved cells. Initially, arriving lipids are rapidly packed in LDs that are resistant to starvation (pre-LDs). Pre-LDs are restricted ER microdomains with a stable core of neutral lipids. Subsequently, a first round of"emerging" LDs is nucleated, providing additional lipid storage capacity. Finally, in proportion to lipid concentration, new rounds of LDs progressively assemble. Confocal microscopy and electron tomography suggest that emerging LDs are nucleated in a limited number of ER microdomains after a synchronized stepwise process of protein gathering, lipid packaging, and recognition by Plin3 and Plin2. A comparative analysis demonstrates that the acyl-CoA synthetase 3 is recruited early to the assembly sites, where it is required for efficient LD nucleation and lipid storage.
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Este proyecto tiene como objetivo el diseño y la descripción de la instalación de refrigeración de un conjunto de diez viviendas, de nueva construcción, en la localidad de Fraga (Huesca). La solución a la que ha llegado el autor del proyecto ha sido un sistema de refrigeración centralizado mediante una máquina de refrigeración por absorción, accionada a su vez por agua caliente procedente de un sistema solar térmico de captadores de vacío. La refrigeración de las viviendas se lleva a cabo mediante una instalación de agua fría, utilizando fan-coils como unidades terminales en las estancias de las viviendas.
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Este proyecto resuelve el precario sistema de abastecimiento de agua potable actual de las poblaciones de Térmens y Menàrguens sustituyéndolo por otro nuevo, que utiliza aguas del sistema del canal de Piñana, en concreto de la tubería de abastecimiento a Lleida que actualmente se está ejecutando en su segunda fase. Se llega a la solución mediante dos tuberías de fundición dúctil una para cada localidad que llenan los depósitos ya existentes, además se ponen las bases del sistema de telegestión que deberá integrarse en la red general que se construirá en la fase que se está construyendo en la actualidad.
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Confecció d’un projecte que doni les bases tècniques necessàries per la construcció d’una presa al Barranc del Solà (o de Rivert) per regular el reg d’una superfície aproximada de 500 ha al terme municipal de Salàs de Pallars a la comarca del Pallars Jussà, per la Comunitat de Regants “Sèquia del Molí”.