Effect of expiratory flow limitation on respiratory mechanical impedance: a model study

Large phasic variations of respiratory mechanical impedance (Zrs) have been observed during induced expiratory flow limitation (EFL) (M. Vassiliou, R. Peslin, C. Saunier, and C. Duvivier. Eur. Respir. J. 9: 779-786, 1996). To clarify the meaning of Zrs during EFL, we have measured from 5 to 30 Hz the input impedance (Zin) of mechanical analogues of the respiratory system, including flow-limiting elements (FLE) made of easily collapsible rubber tubing. The pressures upstream (Pus) and downstream (Pds) from the FLE were controlled and systematically varied. Maximal flow (Vmax) increased linearly with Pus, was close to the value predicted from wave-speed theory, and was obtained for Pus-Pds of 4-6 hPa. The real part of Zin started increasing abruptly with flow (V) >85%Vmax and either further increased or suddenly decreased in the vicinity of V¿max. The imaginary part of Zin decreased markedly and suddenly above 95%Vmax. Similar variations of Zin during EFL were seen with an analogue that mimicked the changes of airway transmural pressure during breathing. After pressure andV measurements upstream and downstream from the FLE were combined, the latter was analyzed in terms of a serial (Zs) and a shunt (Zp) compartment. Zs was consistent with a large resistance and inertance, and Zp with a mainly elastic element having an elastance close to that of the tube walls. We conclude that Zrs data during EFL mainly reflect the properties of the FLE.

Thrombin-induced contraction in alveolar epithelial cells probed by traction microscopy

Contractile tension of alveolar epithelial cells plays a major role in the force balance that regulates the structural integrity of the alveolar barrier. The aim of this work was to study thrombin-induced contractile forces of alveolar epithelial cells. A549 alveolar epithelial cells were challenged with thrombin, and time course of contractile forces was measured by traction microscopy. The cells exhibited basal contraction with total force magnitude 55.0 ± 12.0 nN (mean ± SE, n = 12). Traction forces were exerted predominantly at the cell periphery and pointed to the cell center. Thrombin (1 U/ml) induced a fast and sustained 2.5-fold increase in traction forces, which maintained peripheral and centripetal distribution. Actin fluorescent staining revealed F-actin polymerization and enhancement of peripheral actin rim. Disruption of actin cytoskeleton with cytochalasin D (5 µM, 30 min) and inhibition of myosin light chain kinase with ML-7 (10 µM, 30 min) and Rho kinase with Y-27632 (10 µM, 30 min) markedly depressed basal contractile tone and abolished thrombin-induced cell contraction. Therefore, the contractile response of alveolar epithelial cells to the inflammatory agonist thrombin was mediated by actin cytoskeleton remodeling and actomyosin activation through myosin light chain kinase and Rho kinase signaling pathways. Thrombin-induced contractile tension might further impair alveolar epithelial barrier integrity in the injured lung.

A new estimator to minimize the error due to breathing in the measurement of respiratory impedance

This paper presents a new respiratory impedance estimator to minimize the error due to breathing. Its practical reliability was evaluated in a simulation using realistic signals. These signals were generated by superposing pressure and flow records obtained in two conditions: 1) when applying forced oscillation to a resistance- inertance- elastance (RIE) mechanical model; 2) when healthy subjects breathed through the unexcited forced oscillation generator. Impedances computed (4-32 Hz) from the simulated signals with the new estimator resulted in a mean value which was scarcely biased by the added breathing (errors less than 1 percent in the mean R, I , and E ) and had a small variability (coefficients of variation of R, I, and E of 1.3, 3.5, and 9.6 percent, respectively). Our results suggest that the proposed estimator reduces the error in measurement of respiratory impedance without appreciable extracomputational cost.

Shaping mechanisms of metal specificity in a family of metazoan Metallothioneins: evolutionary differentiation of Mollusc MTs.

Background: The degree of metal binding specificity in metalloproteins such as metallothioneins (MTs) can be crucial for their functional accuracy. Unlike most other animal species, pulmonate molluscs possess homometallic MT isoforms loaded with Cu+ or Cd2+. They have, so far, been obtained as native metal-MT complexes from snail tissues, where they are involved in the metabolism of the metal ion species bound to the respective isoform. However, it has not as yet been discerned if their specific metal occupation is the result of a rigid control of metal availability, or isoform expression programming in the hosting tissues or of structural differences of the respective peptides determining the coordinative options for the different metal ions. In this study, the Roman snail (Helix pomatia) Cu-loaded and Cd-loaded isoforms (HpCuMT and HpCdMT) were used as model molecules in order t o elucidate the biochemical and evolutionary mechanisms permitting pulmonate MTs to achieve specificity for their cognate metal ion. Results: HpCuMT and HpCdMT were recombinantly synthesized in the presence of Cd2+, Zn2+ or Cu2+ and corresponding metal complexes analysed by electrospray mass spectrometry and circular dichroism (CD) and ultra violet-visible (UV-Vis) spectrophotometry. Both MT isoforms were only able to form unique, homometallic and stable complexes (Cd6-HpCdMT and Cu12-HpCuMT) with their cognate metal ions. Yeast complementation assays demonstrated that the two isoforms assumed metal-specific functions, in agreement with their binding preferences, in heterologous eukaryotic environments. In the snail organism, the functional metal specificity of HpCdMT and HpCuMT was contributed by metal-specific transcription programming and cell-specific expression. Sequence elucidation and phylogenetic analysis of MT isoforms from a number of snail species revealed that they possess an unspecific and two metal-specific MT isoforms, whose metal specificity was achieved exclusively by evolutionary modulation of non-cysteine amino acid positions. Conclusion: The Roman snail HpCdMT and HpCuMT isoforms can thus be regarded as prototypes of isoform families that evolved genuine metal-specificity within pulmonate molluscs. Diversification into these isoforms may have been initiated by gene duplication, followed by speciation and selection towards opposite needs for protecting copper-dominated metabolic pathways from nonessential cadmium. The mechanisms enabling these proteins to be metal-specific could also be relevant for other metalloproteins.

Analog circuit for real-time computation of respiratory mechanical impedance in sleep studies

The aim of this work was to develop a low-cost circuit for real-time analog computation of the respiratory mechanical impedance in sleep studies. The practical performance of the circuit was tested in six patients with obstructive sleep apnea. The impedance signal provided by the analog circuit was compared with the impedance calculated simultaneously with a conventional computerized system. We concluded that the low-cost analog circuit developed could be a useful tool for facilitating the real-time assessment of airway obstruction in routine sleep studies.

L'assessorament educatiu en la cruïlla de la formació del professorat

El asesoramiento educativo ha sido objeto de gran atención pero a menudo es concebido como una cuestión metodológica basada en modelos que van desde la transmisión estricta de conocimientos a la colaboración entre iguales. El asesoramiento no sólo es una cuestión de sólidos procedimientos sino que también ha de atender el bienestar de las personas. Para ello es importante poder tomar en consideración los procesos de recuperación de los temas como la integración curricular o las identidades y culturas docentes. Además, la mirada de la autora se detiene en el espacio demarcado por la relación entre la escuela y quienes trabajan en ella. Una escuela que necesita abrirse al entorno y que abra vías reales de participación a la comunidad. Desde este lugar la autora expone las dificultades que atraviesa el asesoramiento en las escuelas ligadas a la necesidad de actualizar el sentido del currículum. Así el poder dar sentido a lo que hacemos y a la revisión de nuestros propósitos no es un atributo que llega mágicamente del exterior sino que resulta de un largo entramado de intercambios de opiniones que nos permiten jugar entre lo nuevo y lo viejo, lo conocido y lo desconocido, lo deseable y lo posible, etc. El asesoramiento no surge, pues, de la transmisión sino de la implicación, y constituye una de las tareas más relevantes de la formación del profesorado.

Opinions al voltant de les relacions entre l'economia i l'educació

La influència decisiva que té l'economia en la majoria deis aspectes que configuren la vida humana és una evidencia, i l'educació no constitueix cap excepció. Philip H. Combs, prestigiós expert en temes relacionats amb els problemes educatius en general i més concretament en la seva dimensió econòmica i social, en analitzar els factors que caracteritzaven la crisi educativa a escala mundial a partir deis anys seixanta, va destacar com a essencials: el desbordament estudiantil (augment de la demanda). L'aguda escassesa de recursos, l'augment deis costos, la inadaptació del producte i la inercia i ineficiencia.

La promoció de la lectura a Catalunya (2008-2009)

D'un temps ençà, la preocupació pels baixos índexs lectors de la població juntament amb els lamentables resultats de les proves PISA de comprensió lectora entre els escolars, han portat les administracions i organismes que en depenen, com biblioteques i escoles, a augmentar els esforços en el camp de la promoció lectora. La revisió d'aquest àmbit s'articula en tres blocs: en el primer, es fa balanç de les accions més significatives dutes a terme els anys 2008 i 2009 des del govern autonòmic i dels governs municipals, les activitats de promoció lectura que s'han dut a terme a les biblioteques públiques i les iniciatives dutes a terme des de les biblioteques escolars arran de la posta en marxa del Puntedu i de l'elaboració de plans de lectura de centre. En un segon bloc, es recullen les iniciatives a l'entorn de la recerca i la formació, molt menors en quantitat. I en el tercer bloc, s'inclou una bibliografia seleccionada de tot tipus de documents¿llibres, articles de revistes, actes de congressos i llocs web¿ sobre la promoció de la lectura publicats durant els anys 2008 i 2009, de la qual se'n comenten alguns títols.

La promoció de la lectura a Catalunya (2008-2009)

D'un temps ençà, la preocupació pels baixos índexs lectors de la població juntament amb els lamentables resultats de les proves PISA de comprensió lectora entre els escolars, han portat les administracions i organismes que en depenen, com biblioteques i escoles, a augmentar els esforços en el camp de la promoció lectora. La revisió d'aquest àmbit s'articula en tres blocs: en el primer, es fa balanç de les accions més significatives dutes a terme els anys 2008 i 2009 des del govern autonòmic i dels governs municipals, les activitats de promoció lectura que s'han dut a terme a les biblioteques públiques i les iniciatives dutes a terme des de les biblioteques escolars arran de la posta en marxa del Puntedu i de l'elaboració de plans de lectura de centre. En un segon bloc, es recullen les iniciatives a l'entorn de la recerca i la formació, molt menors en quantitat. I en el tercer bloc, s'inclou una bibliografia seleccionada de tot tipus de documents¿llibres, articles de revistes, actes de congressos i llocs web¿ sobre la promoció de la lectura publicats durant els anys 2008 i 2009, de la qual se'n comenten alguns títols.

Financial reforms and capital flows: Insights from general equilibrium

As a result of debt enforcement problems, many high-productivity firms in emergingeconomies are unable to pledge enough future profits to their creditors and this constrains thefinancing they can raise. Many have argued that, by relaxing these credit constraints, reformsthat strengthen enforcement institutions would increase capital flows to emerging economies. Thisargument is based on a partial equilibrium intuition though, which does not take into account theorigin of any additional resources that flow to high-productivity firms after the reforms. We showthat some of these resources do not come from abroad, but instead from domestic low-productivityfirms that are driven out of business as a result of the reforms. Indeed, the resources released bythese low-productivity firms could exceed those absorbed by high-productivity ones so that capitalflows to emerging economies might actually decrease following successful reforms. This resultprovides a new perspective on some recent patterns of capital flows in industrial and emergingeconomies.

Transversalitat i participació juvenil : estratègies per promoure que els joves esdevinguin agents actius en les polítiques locals de joventut : proposta d’intervenció al municipi de Caldes de Malavella

Proposta metodològica que té com a element central la participació juvenil. La participació entesa com l’impuls d’estructures democràtiques entre els joves. Aquest treball està centrat en el manteniment de la consolidació de les polítiques de joventut al municipi de Caldes de Malavella. La pèrdua de recursos econòmics és un element que dificulta la realització de projectes que incideixin de manera positiva en els joves. La creació d’estructures de participació entre els joves és una manera de motivar als joves i fer-los membres actius en el desenvolupament de les polítiques locals

Gorilla genome structural variation reveals evolutionary parallelisms with chimpanzee

Structural variation has played an important role in the evolutionary restructuring of human and great ape genomes. Recent analyses have suggested that the genomes of chimpanzee and human have been particularly enriched for this form of genetic variation. Here, we set out to assess the extent of structural variation in the gorilla lineage by generating 10-fold genomic sequence coverage from a western lowland gorilla and integrating these data into a physical and cytogenetic framework of structural variation. We discovered and validated over 7665 structural changes within the gorilla lineage, including sequence resolution of inversions, deletions, duplications, and mobile element insertions. A comparison with human and other ape genomes shows that the gorilla genome has been subjected to the highest rate of segmental duplication. We show that both the gorilla and chimpanzee genomes have experienced independent yet convergent patterns of structural mutation that have not occurred in humans, including the formation of subtelomeric heterochromatic caps, the hyperexpansion of segmental duplications, and bursts of retroviral integrations. Our analysis suggests that the chimpanzee and gorilla genomes are structurally more derived than either orangutan or human genomes.

Sequencing human-gibbon breakpoints of synteny reveals mosaic new insertions at rearrangement sites

The gibbon genome exhibits extensive karyotypic diversity with an increased rate of chromosomal rearrangements during evolution. In an effort to understand the mechanistic origin and implications of these rearrangement events, we sequenced 24 synteny breakpoint regions in the white-cheeked gibbon (Nomascus leucogenys, NLE) in the form of high-quality BAC insert sequences (4.2 Mbp). While there is a significant deficit of breakpoints in genes, we identified seven human gene structures involved in signaling pathways (DEPDC4, GNG10), phospholipid metabolism (ENPP5, PLSCR2), beta-oxidation (ECH1), cellular structure and transport (HEATR4), and transcription (ZNF461), that have been disrupted in the NLE gibbon lineage. Notably, only three of these genes show the expected evolutionary signatures of pseudogenization. Sequence analysis of the breakpoints suggested both nonclassical nonhomologous end-joining (NHEJ) and replication-based mechanisms of rearrangement. A substantial number (11/24) of human-NLE gibbon breakpoints showed new insertions of gibbon-specific repeats and mosaic structures formed from disparate sequences including segmental duplications, LINE, SINE, and LTR elements. Analysis of these sites provides a model for a replication-dependent repair mechanism for double-strand breaks (DSBs) at rearrangement sites and insights into the structure and formation of primate segmental duplications at sites of genomic rearrangements during evolution.