Effects of a post-weaning cafeteria diet in young rats: metabolic syndrome, reduced activity and low anxiety-like behaviour.

Among adolescents, overweight, obesity and metabolic syndrome are rapidly increasing in recent years as a consequence of unhealthy palatable diets. Animal models of diet-induced obesity have been developed, but little is known about the behavioural patterns produced by the consumption of such diets. The aim of the present study was to determine the behavioural and biochemical effects of a cafeteria diet fed to juvenile male and female rats, as well as to evaluate the possible recovery from these effects by administering standard feeding during the last week of the study. Two groups of male and female rats were fed with either a standard chow diet (ST) or a cafeteria (CAF) diet from weaning and for 8 weeks. A third group of males (CAF withdrawal) was fed with the CAF diet for 7 weeks and the ST in the 8th week. Both males and females developed metabolic syndrome as a consequence of the CAF feeding, showing overweight, higher adiposity and liver weight, increased plasma levels of glucose, insulin and triglycerides, as well as insulin resistance, in comparison with their respective controls. The CAF diet reduced motor activity in all behavioural tests, enhanced exploration, reduced anxiety-like behaviour and increased social interaction; this last effect was more pronounced in females than in males. When compared to animals only fed with a CAF diet, CAF withdrawal increased anxiety in the open field, slightly decreased body weight, and completely recovered the liver weight, insulin sensitivity and the standard levels of glucose, insulin and triglycerides in plasma. In conclusion, a CAF diet fed to young animals for 8 weeks induced obesity and metabolic syndrome, and produced robust behavioural changes in young adult rats, whereas CAF withdrawal in the last week modestly increased anxiety, reversed the metabolic alterations and partially reduced overweight.

Multi-scale effects of nestling diet on breeding performance in a terrestrial top predator inferred from stable isotope analysis

Inter-individual diet variation within populations is likely to have important ecological and evolutionary implications. The diet-fitness relationships at the individual level and the emerging population processes are, however, poorly understood for most avian predators inhabiting complex terrestrial ecosystems. In this study, we use an isotopic approach to assess the trophic ecology of nestlings in a long-lived raptor, the Bonelli"s eagle Aquila fasciata, and investigate whether nestling dietary breath and main prey consumption can affect the species" reproductive performance at two spatial scales: territories within populations and populations over a large geographic area. At the territory level, those breeding pairs whose nestlings consumed similar diets to the overall population (i.e. moderate consumption of preferred prey, but complemented by alternative prey categories) or those disproportionally consuming preferred prey were more likely to fledge two chicks. An increase in the diet diversity, however, related negatively with productivity. The age and replacements of breeding pair members had also an influence on productivity, with more fledglings associated to adult pairs with few replacements, as expected in long-lived species. At the population level, mean productivity was higher in those population-years with lower dietary breadth and higher diet similarity among territories, which was related to an overall higher consumption of preferred prey. Thus, we revealed a correspondence in diet-fitness relationships at two spatial scales: territories and populations. We suggest that stable isotope analyses may be a powerful tool to monitor the diet of terrestrial avian predators on large spatio-temporal scales, which could serve to detect potential changes in the availability of those prey on which predators depend for breeding. We encourage ecologists and evolutionary and conservation biologists concerned with the multi-scale fitness consequences of inter-individual variation in resource use to employ similar stable isotope-based approaches, which can be successfully applied to complex ecosystems such as the Mediterranean.

El plom ibèric escrit del Tossal del Mor (Tàrrega, Urgell)

[spa]El presente trabajo culmina un proyecto dedicado al estudio de cuatro láminas de plomo con escritura ibérica procedentes del área ilergeta, que habñian sido extraídas de forma irregular de forma irregular a principios de la década de los años 80 del pasado siglo y que acabaron en el Instituto de Estudios Ilerdenses. En 2005 se dio a conocer el plomo de Olriols (Sant Esteve de Llitera), en 2009 los plomos 1 y 2 de Monteró (Camarasa) y ahora finalmente el plomo del Tossal del Mor (Tárrega). Este texto es muy corto, sólo consta de cuatro segmentos, todos posiblemete antropónimos sin ningún morfo añadido. Desde el punto de vista paleográfico, es significativo que por primera vez se documente un signo dual ko complejo en la zona ilergeta. Mientras que del léxico, cabe destacar que en uno de los antroponimos se documenta por primera vez con claridad en una inscripción ibérica el elemento iber. Independientemente de su significado parece plausible su relación con el etnónimo o con el río con los que antiguos griegos y romanos identificaban a los íberos. [eng]This work is the culmination of a project dedicated to the study of four sheets of lead with Iberian writing from the Ilerget area that had been extracted without authorisation in the early 1980s and that found way to the Institut d"Estudis Ilerdencs (Lleida). In 2005, the lead from Olriols (Sant Esteve de Llitera) was presented, as were lead sheets 1 and 2 from Monteró (Camarasa) in 2009 and now finally the lad piece from Tossal del Mor (Tàrrega). The text is very short, with only four segments, all possibly anthroponyms without any added morph. From the palaeographic point of view, it is significant that, for the first time, a complex dual ko sign is documented in the Ilerget area. Regarding the lexis, it is of note that in one of the anthroponyms the element ibeŕ is documented clearly for the first time in an Iberian inscription. Independently of its meaning, it seems plausible to link it to the ethnonym or the river with which the ancient Greeks and Romans identified the Iberians.

D’espai per a llibres a espai per a usuaris: una antiga caserna militar com a resposta a la manca d’espai

A començament del segle XXI algunes biblioteques universitàries catalanes van detectar una necessitat per la manca d'espais i la reconversió de les biblioteques físiques dins el nou panorama educatiu europeu. Amb el mateix esperit de cooperació que caracteritzava anteriors programes i serveis del CBUC (Consorci de Biblioteques Universitàries de Catalunya), el Consorci va engegar un projecte per fer front a aquesta necessitat. Un estudi inicial va ser encarregat el 2002 i el 2003 es va trobar un emplaçament adequat (una antiga caserna militar) a Lleida. La inauguració oficial va tenir lloc el 2008. El GEPA (Garantia d'Espai per a la Preservació de l'Accés), és un magatzem cooperatiu que té com a objectius conservar els documents de baix ús, garantint la seva preservació futura i la seva accessibilitat immediata quan alguna biblioteca ho requereixi, convertir espai per a llibres en espais per als usuaris de les biblioteques, i fer-ho estalviant espai i diners. El document presenta una breu introducció històrica sobre la gestió física de les col·leccions de les biblioteques, i una breu descripció dels dipòsits de biblioteca d'alta densitat d’arreu del món com a resposta a l’urgent problema de la manca d'espais. Els principals objectius de la comunicació són comentar el projecte arquitectònic i les seves implicacions bibliotecàries, i mostrar com l’equipament GEPA ha permès l’adaptació dels espais a les biblioteques universitàries de Catalunya. D'una banda, el document tracta la selecció d'un vell edifici que havia de ser reformat, la determinació de les necessitats bibliotecàries, el sistema de d’emmagatzematge en compacte triat per guardar els documents a l'edifici, la relació entre l'espai físic i la gestió de la informació, i la logística necessària per a la càrrega de documents de baix ús de les biblioteques cap a l’equipament. D'altra banda, mostrarem alguns exemples dels canvis físics a biblioteques catalanes després de càrregues de documents al GEPA.

From room for books to room for users: an old infantry barrack as an answer to the lack of space

At the beginning of the 21st century, some Catalan university libraries detected a need stemming from the lack of space and the reconversion of physical libraries within the new European educational panorama. With the same cooperative spirit that characterized previous CBUC (Consortium of Academic Libraries of Catalonia) programs and services, the Consortium set in motion a project to address this need. An initial study was commissioned in 2002, and in 2003 a suitable building (old infantry barracks) was found in Lleida. The official opening took place in 2008. GEPA (Guaranteed Space for the Preservation of Access) facility is a cooperative repository, whose objectives are to store and preserve low use documents, ensuring their future access when needed, to convert room for books into room for library users, and doing it saving both space and money. The paper presents a brief historical introduction about the physical management of collections in libraries, and a short overview about high density library repositories all over the world, as an answer to the pressing problem of lack of spaces. The main goals of the communication are to comment the architectural project and its librarian issues, and to show how the GEPA facility allowed to change the spaces in university libraries in Catalonia. On the one hand, the paper deals with the selection of an old building to be renovated, the determination of the librarian needs, the compact shelving system chosen to store the documents in the building, the relation between physical space and information management, and the logistics involved in the load of low use documents from the libraries into the facility. On the other hand, we will show some examples of physical changes in Catalan libraries after large loads of documents to GEPA.

Use and availability of scholarly journals in Catalan academic libraries

This paper presents the results of a survey of academics affiliated to the universities that are members of the Consortium of Academic Libraries of Catalonia (CBUC) and an analysis of the availability in the libraries of these universities of the references cited in a sample of articles published by these academics. The results reflect the major importance that researchers assign to scholarly journals as the main source of scientific information. Most state that they use electronic journals either exclusively or in any case more than print journals, a preference that is higher among younger scholars. With regard to frequency of reading, four out of ten researchers state that they read journals virtually every day, while nine out of ten report that they do so at least once a week. Scholars claim that the obstacles they face when trying to download an article are related to the lack of subscriptions. However, the availability study showed that most of the articles cited in their publications are available at least at one of the CBUC libraries. Though most researchers keep a copy of the articles they consult, just over a third of them use some kind of reference management software.

The SUD1 Gene Encodes a Putative E3 Ubiquitin Ligase and Is a Positive Regulator of 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase Activity in Arabidopsis

The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) enzyme catalyzes the major rate-limiting step of the mevalonic acid (MVA) pathway from which sterols and other isoprenoids are synthesized. In contrast with our extensive knowledge of the regulation of HMGR in yeast and animals, little is known about this process in plants. To identify regulatory components of the MVA pathway in plants, we performed a genetic screen for second-site suppressor mutations of the Arabidopsis thaliana highly drought-sensitive drought hypersensitive2 (dry2) mutant that shows decreased squalene epoxidase activity. We show that mutations in SUPPRESSOR OF DRY2 DEFECTS1 (SUD1) gene recover most developmental defects in dry2 through changes in HMGR activity. SUD1 encodes a putative E3 ubiquitin ligase that shows sequence and structural similarity to yeast Degradation of a factor (Doa10) and human TEB4, components of the endoplasmic reticulum-associated degradation C (ERAD-C) pathway. While in yeast and animals, the alternative ERAD-L/ERAD-M pathway regulates HMGR activity by controlling protein stability, SUD1 regulates HMGR activity without apparent changes in protein content. These results highlight similarities, as well as important mechanistic differences, among the components involved in HMGR regulation in plants, yeast, and animals.

Common Variants of TLR1 Associate with Organ Dysfunction and Sustained Pro- Inflammatory Responses during Sepsis

Background: Toll-like receptors (TLRs) are critical components for host pathogen recognition and variants in genes participating in this response influence susceptibility to infections. Recently, TLR1 gene polymorphisms have been found correlated with whole blood hyper-inflammatory responses to pathogen-associated molecules and associated with sepsis-associated multiorgan dysfunction and acute lung injury (ALI). We examined the association of common variants of TLR1 gene with sepsis-derived complications in an independent study and with serum levels for four inflammatory biomarker among septic patients. Methodology/Principal Findings: Seven tagging single nucleotide polymorphisms of the TLR1 gene were genotyped in samples from a prospective multicenter case-only study of patients with severe sepsis admitted into a network of intensive care units followed for disease severity. Interleukin (IL)-1 b, IL-6, IL-10, and C-reactive protein (CRP) serum levels were measured at study entry, at 48 h and at 7th day. Alleles -7202G and 248Ser, and the 248Ser-602Ile haplotype were associated with circulatory dysfunction among severe septic patients (0.001<=p <= 0.022), and with reduced IL-10 (0.012<= p <=0.047) and elevated CRP (0.011<= p <=0.036) serum levels during the first week of sepsis development. Additionally, the -7202GG genotype was found to be associated with hospital mortality (p =0.017) and ALI (p =0.050) in a combined analysis with European Americans, suggesting common risk effects among studies Conclusions/Significance: These results partially replicate and extend previous findings, supporting that variants of TLR1 gene are determinants of severe complications during sepsis.

La Ansiedad como estigma: el estereotipo de la persona ansiosa en la población clínica, sanitaria y general

El objetivo principal de esta investigación fue la evaluación de la teoría implícita o estereotipo que diferentes grupos de población tienen de las personas con ansiedad clínica. Para ello se administró un listado de adjetivos para la heteroevaluación de la persona ansiosa a un total de 400 participantes, distribuidos en cuatro grupos: pacientes con trastorno de ansiedad, población general, médicos y diplomados en enfermería. A los pacientes ansiosos se les volvió a administrar el listado de adjetivos para su autoevaluación. Se llevó a cabo un estudio descriptivo mediante la prueba de χ2 para determinar las diferencias entre grupos en relación a los adjetivos más utilizados. Seguidamente se realizaron dos ANOVAs para establecer diferencias entre grupos a partir de la hetero-evaluación y para comprobar si había diferencias entre la heteroevaluación y la autoevaluación de las personas con trastornos de ansiedad. Los resultados muestran diferencias en la percepción de las personas ansiosas por parte de los 4 grupos; pero solo en el grupo general se detectan creencias prejuiciosas en el estereotipo que muestran de las personas ansiosas. Dichos hallazgos ponen de manifiesto las diferencias en los estereotipos de cada población referentes a las personas con ansiedad clínica

Self-Renewing Human Bone Marrow Mesenspheres Promote Hematopoietic Stem Cell Expansion.

Strategies for expanding hematopoietic stem cells (HSCs) include coculture with cells that recapitulate their natural microenvironment, such as bone marrow stromal stem/progenitor cells (BMSCs). Plastic-adherent BMSCs may be insufficient to preserve primitive HSCs. Here, we describe a method of isolating and culturing human BMSCs as nonadherent mesenchymal spheres. Human mesenspheres were derived from CD45- CD31- CD71- CD146+ CD105+ nestin+ cells but could also be simply grown from fetal and adult BM CD45--enriched cells. Human mesenspheres robustly differentiated into mesenchymal lineages. In culture conditions where they displayed a relatively undifferentiated phenotype, with decreased adherence to plastic and increased self-renewal, they promoted enhanced expansion of cord blood CD34+ cells through secreted soluble factors. Expanded HSCs were serially transplantable in immunodeficient mice and significantly increased long-term human hematopoietic engraftment. These results pave the way for culture techniques that preserve the self-renewal of human BMSCs and their ability to support functional HSCs.