Disturbance, age and size structure in stands of Pinus uncinata Ram

Las estructuras de tamaño y edad de dos bosques monoespecificos subalpinos de Pinus uncinata en el Pirineo, fueron estudiadas e interpretadas introduciendo una clasificación sencilla de los árboles según la exposición de su copa a la radiación solar directa. Ambas poblaciones muestran distribuciones de diámetro de J invertida irregulares, cuya Interpretación es incierta. Por el contrario, la estructura de edad en ambos bosques muestra picos visibles, los cuales, si se tienen en cuenta la exposición de la copa, indican que la regeneración se produce en claros del bosque. Se analizaron las series de anillos de crecimiento de los árboles de cada sitio para reconstruir su historia reciente de perturbaciones Identificando los incrementos bruscos del crecimiento. El régimen de perturbaciones en los dos bosques es diferente, y en ambos casos muestra un efecto madelador análogo sobre la estructura de edad. Se concluye que P. uncinata se comporta como una especie heliófila que puede formar bosques maduros o multicohorte bajo determinados regímenes de perturbaciones suaves, gracias a su capacidad para iniciar la regeneración en claros pequeños. Este proceso tiene lugar en un lapso de tiempo corto después de la incidencia de las perturbaciones.

Reconstructing snow avalanches in the Southeastern Pyrenees

A regional study of snow avalanche processes was undertaken in the SE Pyrenees. Dendrogeomorphology was used to date and reconstruct large-scale snow avalanche events that occurred in the last four decades. Dendrochronological analyses yielded the dates of nine winters when avalanches occurred in the recent past in six studied avalanche paths. Some of these avalanches were already known, but others had not been documented. In one case, the existing avalanche path map was improved with the dendrogeomorphological information of a larger past event. As a result of the dendrogeomorphological analyses, evidence for three regional-scale major avalanche years was identified in the SE Pyrenees from 1971 to 2004: 1971¿1972, 1995¿1996 and 2002¿2003. The specific synoptic atmospheric situations and the most likely nivometeorological and snowpack conditions that released these major avalanches were determined using weather data for the seasons of major avalanche releases. In 1971¿1972 the snow avalanche episode was characterized by a deep trough crossing the Pyrenees. In 1995¿1996 a variety of meteorological situations produced several episodes of major avalanches. In 2002¿2003 the more significant of two episodes was attributed to a north advection pumping an arctic air mass over the Pyrenees. The 1995¿1996 avalanche season proved to be the most notable in the four past decades in the Pyrenees.

The subantartic Nothofagus forests of Tierra del Fuego: distribution, structure and production

Evergreen Nothofagus betuloides and decidoues N. pumilo form the main forest types in Tierra del Fuego. These forest were sampled along two altitudinal gradients to study their structure and dynamics and assess the causes of their distribution.

The subantartic Nothofagus forests of Tierra del Fuego: distribution, structure and production

Evergreen Nothofagus betuloides and decidoues N. pumilo form the main forest types in Tierra del Fuego. These forest were sampled along two altitudinal gradients to study their structure and dynamics and assess the causes of their distribution.

Spatial variability of tree hight at treeline ecotones in the Pyrenees.

We describe the spatial distribution of tree height of Pinus uncinata at two undisturbed altitudinal treeline ecotones in the southern Pyrenees (Ordesa, O, and Tessó, T). At each site, a rectangular plot (30 x 140 m) was located with its longest side parallel to the slope and encompassing treeline and timberline. At site O, height increased abruptly going downslope with a high spatial autocorrelation at short distances. In contrast, the changes of tree height across the ecotone at site T were gradual, and tree height was less spatially autocorrelated. These results can be explained by the greater importance of wind and snow avalanches at sites O and T, respectively.

Estructura espacial de un ecotono bosque subalpino-pastos alpinos en los Estanys de la Pera (Pirineos orientales) formado por manchas de árboles

Se describe la estructura y el patrón espacial de un ecotono bosque subalpino-pastos alpinos dominado por Pinus uncinata Ram. (Pirineos Orientales, Cerdanya, Estanys de la Pera). La historia de perturbaciones de la zona sugiere que el patrón espacial del ecotono puede haber sido afectado recientemente por el pastoreo. Se situó una parcela rectangular (30 x 100 m) cruzando el ecotono con su lado mayor paralelo a la pendiente. Para cada individuo de P. uncinata dentro de la parcela se midió: su localización (coordenadas x, y), y diversas variables estructurales (diámetros basal y a 1,3 m de altura, altura, radios de las copas, número de cohortes de acículas, edad estimada -número de internodos del tronco). Los individuos vivos de P. uncinata se clasificaron según su tamaño (adultos, jóvenes, vástagos y plántulas) y forma de crecimiento (krummholz -individuos arbustivos policórmicos-). Se describieron cuantitativamente el tipo de sustrato (roca, materia orgánica, suelo) y la cobertura de herbáceas, arbustos y P. uncinata usando transectos paralelos a la pendiente. La estructura del ecotono se describió mediante: (i) los análisis del patrón de puntos (K de Ripley, casos uni- y bivariante) y (ii) del patrón de superficies (correlogramas omni- y unidireccionales de Moran). El ecotono estudiado mostró una distribución de individuos de P. uncinata en agregados, predominando los vástagos. Los análisis espaciales mostraron la existencia y el tamaño de agregados con valores similares para ciertas variables de los árboles situados dentro del bosque (p. ej. 8-10 m para la edad estimada). La edad estimada de los individuos jóvenes (60 años <) estaba positivamente correlacionada con la altura y el diámetro basal. La regeneración reciente presentó dos máximos (1965-67, 1973-77). Apareció una posible cohorte de individuos más viejos (1941-57). A partir de 1975, la regeneración declinó de forma exponencial. El establecimiento...

La sucesión en los bosques de Pinus uncinata del Pirineo. De los anillos de crecimiento a la historia del bosque

El patrón de sucesión a largo plazo en los bosques subalpinos de Pinus uncinata y su relación con el régimen de perturbaciones al que se ven sometidos se analizan aquí, tomando como referencia el modelo de sucesión de PEET & CHRISTENSEN (1987). Por métodos dendrocronológicos, utilizando datos de árboles vivos y muertos, se han reconstruido los últimos 140-220 años de la historia de tres bosques suficientemente viejos y poco alterados, al menos recientemente, por el hombre. Los resultados ponen de manifiesto ciertas regularidades importantes en la secuencia y en la duración de las fases de la sucesión observadas en la escala espacial que nos permite el muestreo realizado (un transecto lineal de 280-350 metros. De una larga fase de iniciación (110140 años) se pasa casi directamente a una fase de transición, con escasa evidencia de una fase intermedia de exclusión. Este patrón de sucesión, causado por la lentitud y la heterogeneidad espacial de la fase de iniciación y por un régimen de pequeñas perturbaciones dispersas y frecuentes que aparecen muy pronto, es similar al observado en otros bosques subalpinos y ambientes extremos y representa una desviación respecto del modelo de referencia. La tasa media de mortalidad natural de los áboles adultos durante las últimas 4-6 décadas ha sido, respectivamente para los tres bosques, de 6,2, 1,4 y 5,7% por década. Estas tasas son lo sufientemente bajas como para permitir una larga persistencia en la fase de transición de la cohorte dominante - la instalada durante la fase de iniciación -, en declive pero al mismo tiempo inhibiendo la aparición masiva de regeneración. Ello hace previsible una dinámica de la estructura y la funcionalidad del bosque muy fluctuante a largo plazo en la escala espacial estudiada, aunque en su origen puede encontrarse la mano del hombre a través de una perturbación inicial homogeneizadora

Formación del anillo de crecimiento y clima: un ejemplo de modelos para Pinus uncinata Ram. y Pinus sylvestris L. en los Pirineos Centrales

En este trabajo, describimos el desarrollo y la estructura del anillo de crecimiento de Pinus uncinata Ram. y de Pinus sylvestris L. en un gradiente altitudinal en los Pirineos centralese

Insulin-Like growth-factor 1 myocardial expression decreases in chronic alcohol consumption

Background Chronic alcohol ingestion may cause severe biochemical and pathophysiological derangements to skeletal muscle. Unfortunately, these alcohol-induced events may also prime skeletal muscle for worsened, delayed, or possibly incomplete repair following acute injury. As alcoholics may be at increased risk for skeletal muscle injury, our goals were to identify the effects of chronic alcohol ingestion on components of skeletal muscle regeneration. To accomplish this, age- and gender-matched C57Bl/6 mice were provided normal drinking water or water that contained 20% alcohol (v/v) for 1820 wk. Subgroups of mice were injected with a 1.2% barium chloride (BaCl2) solution into the tibialis anterior (TA) muscle to initiate degeneration and regeneration processes. Body weights and voluntary wheel running distances were recorded during the course of recovery. Muscles were harvested at 2, 7 or 14 days post-injection and assessed for markers of inflammation and oxidant stress, fiber cross-sectional areas, levels of growth and fibrotic factors, and fibrosis. Results Body weights of injured, alcohol-fed mice were reduced during the first week of recovery. These mice also ran significantly shorter distances over the two weeks following injury compared to uninjured, alcoholics. Injured TA muscles from alcohol-fed mice had increased TNFα and IL6 gene levels compared to controls 2 days after injury. Total protein oxidant stress and alterations to glutathione homeostasis were also evident at 7 and 14 days after injury. Ciliary neurotrophic factor (CNTF) induction was delayed in injured muscles from alcohol-fed mice which may explain, in part, why fiber cross-sectional area failed to normalize 14 days following injury. Gene levels of TGFβ1 were induced early following injury before normalizing in muscle from alcohol-fed mice compared to controls. However, TGFβ1 protein content was consistently elevated in injured muscle regardless of diet. Fibrosis was increased in injured, muscle from alcohol-fed mice at 7 and 14 days of recovery compared to injured controls. Conclusions Chronic alcohol ingestion appears to delay the normal regenerative response following significant skeletal muscle injury. This is evidenced by reduced cross-sectional areas of regenerated fibers, increased fibrosis, and altered temporal expression of well-described growth and fibrotic factors.

Variability of the radius growth at 25 years old Pinus uncinata Miller ex Mirbel in the North-East Spanish Pyrenees

It has been studied the variability of stem radial growth in four radius orientations at the age of 25 years old trees. The species was Pinus uncinata. The sampling sites, located in the North East Spanish Pyrenees belong to different plant communities. An ANOVA analysis was performed in order to find out which factors, the radio orientation, plant community of the site and tree itself are significative or have some kind of influence on the tree radial growth.

Reduction of oxidative cellular damage by overexpression of the thioredoxin TRX2 gene improves yield and quality of wine yeast dry active biomass

Background: Wine Saccharomyces cerevisiae strains, adapted to anaerobic must fermentations, suffer oxidative stress when they are grown under aerobic conditions for biomass propagation in the industrial process of active dry yeast production. Oxidative metabolism of sugars favors high biomass yields but also causes increased oxidation damage of cell components. The overexpression of the TRX2 gene, coding for a thioredoxin, enhances oxidative stress resistance in a wine yeast strain model. The thioredoxin and also the glutathione/glutaredoxin system constitute the most important defense against oxidation. Trx2p is also involved in the regulation of Yap1p-driven transcriptional response against some reactive oxygen species. Results: Laboratory scale simulations of the industrial active dry biomass production process demonstrate that TRX2 overexpression increases the wine yeast final biomass yield and also its fermentative capacity both after the batch and fed-batch phases. Microvinifications carried out with the modified strain show a fast start phenotype derived from its enhanced fermentative capacity and also increased content of beneficial aroma compounds. The modified strain displays an increased transcriptional response of Yap1p regulated genes and other oxidative stress related genes. Activities of antioxidant enzymes like Sod1p, Sod2p and catalase are also enhanced. Consequently, diminished oxidation of lipids and proteins is observed in the modified strain, which can explain the improved performance of the thioredoxin overexpressing strain. Conclusions: We report several beneficial effects of overexpressing the thioredoxin gene TRX2 in a wine yeast strain. We show that this strain presents an enhanced redox defense. Increased yield of biomass production process in TRX2 overexpressing strain can be of special interest for several industrial applications.

Engineered Trx2p industrial yeast strain protects glycolysis and fermentation proteins from oxidative carbonylation during biomass propagation

Background: In the yeast biomass production process, protein carbonylation has severe adverse effects since it diminishes biomass yield and profitability of industrial production plants. However, this significant detriment of yeast performance can be alleviated by increasing thioredoxins levels. Thioredoxins are important antioxidant defenses implicated in many functions in cells, and their primordial functions include scavenging of reactive oxygen species that produce dramatic and irreversible alterations such as protein carbonylation. Results: In this work we have found several proteins specifically protected by yeast Thioredoxin 2 (Trx2p). Bidimensional electrophoresis and carbonylated protein identification from TRX-deficient and TRX-overexpressing cells revealed that glycolysis and fermentation-related proteins are specific targets of Trx2p protection. Indeed, the TRX2 overexpressing strain presented increased activity of the central carbon metabolism enzymes. Interestingly, Trx2p specifically preserved alcohol dehydrogenase I (Adh1p) from carbonylation, decreased oligomer aggregates and increased its enzymatic activity. Conclusions: The identified proteins suggest that the fermentative capacity detriment observed under industrial conditions in T73 wine commercial strain results from the oxidative carbonylation of specific glycolytic and fermentation enzymes. Indeed, increased thioredoxin levels enhance the performance of key fermentation enzymes such as Adh1p, which consequently increases fermentative capacity.

Dendrochronological study of the avalanche path Canal del Roc Roig: first results of the ALUDEX Project in the Pyrenees

Historical data about ancient avalanches are scarce in the Pyrenees. Dendrochronology can provide new data about past avalanches and their return period, but up to now little research has been carried out with this purpose. The Aludex project aims to obtain information about the frequency and extent of extreme avalanches, using a dendrochronological and a nivo-meteorological approach. In this paper, we present the results of a dendrochronological study of the Canal del Roc Roig avalanche path which was affected by two extreme avalanches in February 1996. This first dendrochronological study has permitted us to assess the tree-ring signals due to avalanche events in 53 out of 132 dated trees. The trees presented a variety of responses to the 1996 avalanche events. It is shown that the type of tree-ring signal depends on tree age. The methodology has proved successful in detecting the 1996 and 1972 documented avalanche events, and provided outstanding evidence of undocumented past events such as one in 1930.

Using vegetation to characterize the avalanche to Canal del Roc Roig, Vall de Núria (Eastern Pyrenees, Spain)

Avalanche hazard maps of high accuracy are difficult to produce. For land-use planning and management purposes, a good knowledge of extreme run-out zones and frequencies of avalanches is required. In the present work, vegetation recognition (especially focused on Pinus uncinata trees) and dendrochronological techniques are used to characterize avalanches that have occurred in historical times, helping to determine both the extent of large or extreme avalanches and their occurrence in time. Vegetation was studied at the Canal del Roc Roig (eastern Pyrenees, Spain) avalanche path. The avalanches descending this path affect the railway that reaches the Vall de Núria resort and the run-up to the opposite slope. During winter 1996, two important avalanches affecting this path were well documented. These are compared with the results of the vegetation study, consisting of an inventory of flora, the recording of vegetation damages along eight transverse profiles at different altitudes on the path and a dendrochronological sampling campaign. The data obtained contributed to a characterization of the predominant snow accumulation in the starting zone, the 1996 avalanches and the range of frequencies of large avalanches. Also, traces of avalanches that increase the path mapped in the avalanche paths map published by the Institut Cartogràfic de Catalunya in 2000 were identified, improving the initial existing information.