Dendritic cells exposed to MVA-based HIV-1 vaccine induce highly functional HIV-1-specific CD8+ T cell responses in HIV-1-infected individuals

Currently, MVA virus vectors carrying HIV-1 genes are being developed as HIV-1/AIDS prophylactic/therapeutic vaccines. Nevertheless, little is known about the impact of these vectors on human dendritic cells (DC) and their capacity to present HIV-1 antigens to human HIV-specific T cells. This study aimed to characterize the interaction of MVA and MVA expressing the HIV-1 genes Env-Gag-Pol-Nef of clade B (referred to as MVA-B) in human monocyte-derived dendritic cells (MDDC) and the subsequent processes of HIV-1 antigen presentation and activation of memory HIV-1-specific T lymphocytes. For these purposes, we performed ex vivo assays with MDDC and autologous lymphocytes from asymptomatic HIV-infected patients. Infection of MDDC with MVA-B or MVA, at the optimal dose of 0.3 PFU/MDDC, induced by itself a moderate degree of maturation of MDDC, involving secretion of cytokines and chemokines (IL1-ra, IL-7, TNF-α, IL-6, IL-12, IL-15, IL-8, MCP-1, MIP-1α, MIP-1β, RANTES, IP-10, MIG, and IFN-α). MDDC infected with MVA or MVA-B and following a period of 48 h or 72 h of maturation were able to migrate toward CCL19 or CCL21 chemokine gradients. MVA-B infection induced apoptosis of the infected cells and the resulting apoptotic bodies were engulfed by the uninfected MDDC, which cross-presented HIV-1 antigens to autologous CD8+ T lymphocytes. MVA-B-infected MDDC co-cultured with autologous T lymphocytes induced a highly functional HIV-specific CD8+ T cell response including proliferation, secretion of IFN-γ, IL-2, TNF-α, MIP-1β, MIP-1α, RANTES and IL-6, and strong cytotoxic activity against autologous HIV-1-infected CD4+ T lymphocytes. These results evidence the adjuvant role of the vector itself (MVA) and support the clinical development of prophylactic and therapeutic anti-HIV vaccines based on MVA-B.

Genetic variants of the FADS gene cluster and ELOVL gene family, colostrums LC-PUFA levels, breastfeeding, and child cognition

Introduction: Breastfeeding effects on cognition are attributed to long-chain polyunsaturated fatty acids (LC-PUFAs), but controversy persists. Genetic variation in fatty acid desaturase (FADS) and elongase (ELOVL) enzymes has been overlooked when studying the effects of LC-PUFAs supply on cognition. We aimed to: 1) to determine whether maternal genetic variants in the FADS cluster and ELOVL genes contribute to differences in LC-PUFA levels in colostrum; 2) to analyze whether these maternal variants are related to child cognition; and 3) to assess whether children's variants modify breastfeeding effects on cognition. Methods: Data come from two population-based birth cohorts (n = 400 mother-child pairs from INMA-Sabadell; and n = 340 children from INMA-Menorca). LC-PUFAs were measured in 270 colostrum samples from INMA-Sabadell. Tag SNPs were genotyped both in mothers and children (13 in the FADS cluster, 6 in ELOVL2, and 7 in ELOVL5). Child cognition was assessed at 14 mo and 4 y using the Bayley Scales of Infant Development and the McCarthy Scales of Children"s Abilities, respectively. Results: Children of mothers carrying genetic variants associated with lower FADS1 activity (regulating AA and EPA synthesis), higher FADS2 activity (regulating DHA synthesis), and with higher EPA/AA and DHA/AA ratios in colostrum showed a significant advantage in cognition at 14 mo (3.5 to 5.3 points). Not being breastfed conferred an 8- to 9-point disadvantage in cognition among children GG homozygote for rs174468 (low FADS1 activity) but not among those with the A allele. Moreover, not being breastfed resulted in a disadvantage in cognition (5 to 8 points) among children CC homozygote for rs2397142 (low ELOVL5 activity), but not among those carrying the G allele. Conclusion: Genetically determined maternal supplies of LC-PUFAs during pregnancy and lactation appear to be crucial for child cognition. Breastfeeding effects on cognition are modified by child genetic variation in fatty acid desaturase and elongase enzymes.

Epidemiology of foodborne Norovirus outbreaks in Catalonia, Spain

Background: Noroviruses are one of the principal biological agents associated with the consumption of contaminated food. The objective of this study was to analyse the size and epidemiological characteristics of foodborne outbreaks of gastroenteritis in Catalonia, a region in the northeast of Spain.Methods: In all reported outbreaks of gastroenteritis associated with food consumption, faecal samples of persons affected were analysed for bacteria and viruses and selectively for parasites. Study variables included the setting, the number of people exposed, age, sex, clinical signs and hospital admissions. The study was carried out from October 2004 to October 2005.Results: Of the 181 outbreaks reported during the study period, 72 were caused by Salmonella and 30 by norovirus (NoV); the incidence rates were 14.5 and 9.9 per 100,000 person-years, respectively. In 50% of the NoV outbreaks and 27% of the bacterial outbreaks (p = 0.03) the number of persons affected was ¿10; 66.7% of NoV outbreaks occurred in restaurants; no differences in the attack rates were observed according to the etiology. Hospitalizations were more common (p = 0.03) in bacterial outbreaks (8.6%) than in NoV outbreaks (0.15%). Secondary cases accounted for 4% of cases in NoV outbreaks compared with 0.3% of cases in bacterial outbreaks (p < 0.001)Conclusion: Norovirus outbreaks were larger but less frequent than bacterial outbreaks, suggesting that underreporting is greater for NoV outbreaks. Food handlers should receive training on the transmission of infections in diverse situations. Very strict control measures on handwashing and environmental disinfection should be adopted in closed or partially-closed institutions.

Foodborne norovirus outbreak: the role of an asymptomatic food handler

Background: In July 2005 an outbreak of acute gastroenteritis occurred on a residential summer camp in the province of Barcelona (northeast of Spain). Forty-four people were affected among residents and employees. All of them had in common a meal at lunch time on 13 July (paella, round of beef and fruit). The aim of this study was to investigate a foodborne norovirus outbreak that occurred in the residential summer camp and in which the implication of a food handler was demonstrated by laboratory tests. Methods: A retrospective cohort study was designed. Personal or telephone interview was carried out to collect demographic, clinical and microbiological data of the exposed people, as well as food consumption in the suspected lunch. Food handlers of the mentioned summer camp were interviewed. Ten stool samples were requested from symptomatic exposed residents and the three food handlers that prepared the suspected food. Stools were tested for bacteries and noroviruses. Norovirus was detected using RT-PCR and sequence analysis. Attack rate, relative risks (RR) and its 95% confidence intervals (CI) were calculated to assess the association between food consumption and disease. Results: The global attack rate of the outbreak was 55%. The main symptoms were abdominal pain (90%), nausea (85%), vomiting (70%) and diarrhoea (42.5%). The disease remitted in 24-48 hours. Norovirus was detected in seven faecal samples, one of them was from an asymptomatic food handler who had not eaten the suspected food (round of beef), but cooked and served the lunch. Analysis of the two suspected foods isolated no pathogenic bacteria and detected no viruses. Molecular analysis showed that the viral strain was the same in ill patients and in the asymptomatic food handler (genotype GII.2 Melksham-like). Conclusions: In outbreaks of foodborne disease, the search for viruses in affected patients and all food handlers, even in those that are asymptomatic, is essential. Health education of food handlers with respect to hand washing should be promoted.

Una nova societat civil: accions col·lectives de masses a l'Espanya postransicional (ca. 1982-2002)

INFORME DE RECERCA: Aquest treball ha consistit en una indagació sobre la protesta socialrecent al nostre país (Espanya, 1982-2002); específicament, sobre els principals episodis contemporànisd'acció col.lectiva que han esclatat a Espanya i a Catalunya, amb dos paràmetresdelimitadors bàsics:a) haver-se produït en el periode postransicional, és a dir, amb la fase de resolució dela transició política ja culminada (cosa que, com altres investigadors, hipotitzemque es produí pel 1982, amb el primer relleu democràtic i pacífic del Govern iamb el que anomenem transició institucional ja acabada); per tant, entre 1982 i2003 aproximadament.b) haver mobilitzat un seguiment massiu i adquirit, degut a aquest motiu, a vegadesreforçat per altres, una significació política excepcional, fora d'allò que esconsidera normal en l'activitat política quotidiana (itenint en conseqüència un impacte molt notable en el decurs mateix delperiode postransicional).

La Enfermería como grupo oprimido: las voces de las protagonistas

Es un estudio etnográfico que tuvo como objetivo analizar las relaciones de poder y dominación desde la perspectiva freireana en la enseñanza de la enfermería y su repercusión en la conformación de una identidad profesional subordinada. Participaron docentes y estudiantes de una de las escuelas universitarias de enfermería españolas y enfermeras asistenciales. Los datos fueron obtenidos por triangulación de métodos: observación participante y no participante y entrevistas. Se registraron en un cuaderno de notas de campo o en cinta magnetofonica y se analizaron mediante el método de las comparaciones constantes. Los resultados muestran mecanismos mediante los cuales las ideologías dominantes en la formación de profesionales de la salud dan forma y contenido a la subjetividad de profesoras y estudiantes dirigiéndolas hacia la conformidad y subordinación para con las prácticas establecidas en las instituciones sanitárias.

Tecnologia i metodologia en l'ensenyament universitari

Aquest article ha estat concebut en dues parts diferenciades, la primera és una aproximació conceptual a l'ús de mitjans en l'ensenyament superior, la segona inclou una analisi crítica de l'ús i de les possibilitats d'aquests mitjans; aquesta analisi es porta a terme des de la perspectiva teórica plantejada anteriorment i amb una dimensió practica i normativa explícita.

Recursos tecnològics i àudio-visuals per a la formació a l'empresa

El director de personal d'una empresa va rebre un informe en que se li suggeria que un important problema de formació podria ser resolt amb l'ajut d'un sistema TEL. Es va plantajar el tema en una reunió de l'equip directiu, pero cap deis assistents no tenia una noció precisa del que significava aixo. Al director de formació li encarregaren d'estudiar la qüestió. Afortunadament, aquells dies se celebraven en una ciutat europea una conferencia i una exhibició sobre TEL, i va decidir d'anar-hi. Després de visitar molts stands, assistir a demostracions i establir diversos contactes, tingué la impressió que era més rellevant la qualitat deis grafics que no pas el contingut de formació; molts deis paquets d'EA0 no eran sinó reculls d'exercicis i molt rarament semblava que s'hi haguessin tingut en compte els principis d'aprenentatge més elementals.

Laboratori de mitjans, estat de la investigació. Grup I més D del Departament de Didàctica i Organització Educativa

M'han sol-licitat una breu presentació del treball que realitzem al grup 1 més 0, que jo coordino. Tractaré de resumir-ne les línies d'investigació en la brevetat de l'espai que m'han destinat. Com que el treball que es presenta és obra de diverses persones, hem inclas les seves adreces electròniques com a referéncies.

La complejidad del cuidado y el cuidado de la complejidad: un tránsito pedagógico de los reduccionismos fragmentantes a las lógicas no lineales de la complejidad

RESUMEN: Este trabajo aborda la formación inicial en enfermería y su práctica profesional utilizando para ello instrumentos conceptuales pertenecientes a lo "paradigma de la complejidad": esquemas teóricos dinámicos, categorías conceptuales ambivalentes utilizados para dar cuenta de las incertidumbres, indeterminaciones y fenómenos aleatorios que conforman los procesos que tienen lugar en la formación inicial de Enfermería y en su ejercicio profesional. Categorías que tratan de ir más allá del reduccionismo positivista que había pretendido eliminar la imprecisión, la ambigüedad y la contradicción, y se sitúan en una perspectiva en la que la paradoja, la dualidad, la incertidumbre o la contradicción que, antes que errores o límites de nuestro pensamiento, son características constitutivas de la realidad del cuidado y de la los procesos formativos que capacitan para su ejercicio profesional. La tesis central de estos planteamientos afirma que las formas de representación positivista han asignado a los fenómenos del cuidado profesional de la salud y a los de su enseñanza una certidumbre de la que realmente carecen.

Evolución tectonoestratigráfica de los Catalánides

Proposem una divisió dels Catalanids en unitats que reflecteixen un comportament paleogeografic i estratigrafic diferent durant l'etapa preorogenica (Mesozoic) i un de tectonic relativament diferenciat durant l'etapa compressiva (Paleoge). Distingim els sectors (extern, intermedi i intern) limitats per falles de sbcol longitudinals, paral.leles a la serralada, la majoria de les quals es concentra en una zona que separa els sectors extern i intern. En aquesta zona (sector intermedi) s'hi atasconen les unitats litoestratigrafiques mesozoiques. Aquest atasconament, conjuntament amb la reactivació de les fractures profundes, és el responsable, durant l'etapa compressiva, de la localització en el sector intermedi dels fronts de les principals estructures d'encavalcament. També distingim les unitats que hem anomenat domini; (septentrional, central i meridional), que estan limitades per fractures transversals a la serralada. Així, doncs, les unitats queden limitades per dos sistemes de fractures de sòcol aproximadament ortogonals.

¿El modelo de cuña compuesta de C.I. Migliorini (1948) es aplicable a la estructura del rector central de los Catalánides?

Se comentan las conclusiones sobre la estructura de dos Catalánides recientemente publicadas, resaltando la ausencia de datos concretos que permitan aplicar el modelo de la cuna compuesta de Migliorini para 1os Catalánides. Además, los datos publicados (previamente, más como nuestras observaciones personales, indican una clara separación en el tiempo de una fase de comprensión y otra de distensión posterior.

Els fongs de Catalunya occidental segons les prospeccions recents. II

This paper contains the conclusion of a mycological survey of western Catalonia. The first part of the results was published in Acta Bot. Barc. 45 (Homenatge a Oriol de Bolòs): 57-89. The present part covers the last two groups of Basidiomycota: the agarics (326 species) and the gasteromycetes (44 species). The data regarding the surveyed area, collection localities, abbreviations used in the information on ecology, collectors and identifiers are found in the mentioned first part, which contains records of 37 species of Myxomycota, 5 of Zygomycota, 101 of mitosporic fungi, 8 of Teliomycetes, 1 of Ustomycetes, 16 of Phragmobasidiomycetes and 92 of Aphyllophorales. Together with the first part, the results of our survey are a useful contribution to an improved understanding of the fungal component of plant communities of the dry, warm lowlands of the western Mediterranean region, and highlight the remarkable reproductive activity of the fungi observed during late Autumn and Winter, in the studied area.

Spore germination and development of young mycelia in some Rhizopogon species

Spore germination in Rhizopogon abietis, R. luteolus, R. roseolus and R. villosulus was induced in the presence of Rhodotorula glutinis and activated charcoal, in agar medium (N6:5). In one R. roseolus sample, 51% of spores germinated within 35 days, allowing observation of the course of spore germination and the different developmental patterns of homokaryotic mycelia. In these plates, spores showed two times of germination. The spores that germinated early produced an apical germ tube. Later other spores germinated in proximity to young mycelium, by forming a germ vesicle. One of the hyphal growth patterns obtained (interruption-swelling-ramification) is similar to that reported for other fungi. With this technique for inducing spore germination, it is possible to obtain enough monosporic cultures to perform mating tests. Key words: Basidiomycotina, Hypogeous, Monosporic Cultures.

Collections in Institutional Herbaria listed as Rhizopogon, but not belonging to this genus

Fifty-five collections misidentified and stored in various institutional herbaria, under severa1 Rhizopogon species narnes, are revised. Among these, twelve belong to Ascomycotina and 9 were identified to species level; the other collections, immature fruitbodies wcre impossible to identify with the data available. Forty-two collections are Basidiomycotina and only an immature Scleroderma remain without identification to the species level.