Gesture based interface for image annotation

Disserta����o apresentada para obten����o do Grau de Mestre em Engenharia Inform��tica pela Universidade Nova de Lisboa, Faculdade de Ci��ncias e Tecnologia

Interfaces for television content sharing and annotation

Disserta����o para obten����o do Grau de Mestre em Engenharia Inform��tica

How different generations contact and search for brands on social media? Comparing gen X and Y

This research intends to examine if there were significant differences on the brand engagement and on the electronic word of mouth (e-WOM)1 referral intention through Facebook between Generation X and Generation Y (also called millennials). Also, this study intends to examine if there are differences in the motivations that drive these generations to interact with brands through Facebook. Results indicated that Generation Y members consumed more content on Facebook brands��� pages than Generation X. Also, they were more likely to have an e-WOM referral intention as well as being more driven by brand affiliation and opportunity seeking. Finally, currently employed individuals were found to contribute with more content than students. This study fills the gap in the literature by addressing how marketing professionals should market their brand and interact and engage with their customers, based on customers��� generational cohort.

Culturtainment - a new interactive entertainment genre: assessment of Cultural Heritage digital content in leisure software games

European Master in Multimedia and Audiovisual Administration

Discovery and retrieval of Geographic data using Google

Dissertation submitted in partial fulfilment of the requirements for the Degree of Master of Science in Geospatial Technologies

Bitocast: a hybrid BitTorrent and IP Multicast content distribution solution

Disserta����o apresentada para obten����o do Grau de Mestre em Inform��tica, pela Universidade Nova de Lisboa, Faculdade de Ci��ncias e Tecnologia

A lightweight distributed super peer election algorithm for unstructured dynamic P2P systems

Disserta����o apresentada na Faculdade de Ci��ncias e Tecnologia da Universidade Nova de Lisboa para obten����o do grau de Mestre em Engenharia Electrot��cnica e de Computadores

Automated image tagging through tag propagation

Trabalho apresentado no ��mbito do Mestrado em Engenharia Inform��tica, como requisito parcial Para obten����o do grau de Mestre em Engenharia Inform��tica

Genetics of berry colour and anthocyanin content variation in grapevine (Vitis vinifera L.subsp. vinifera)

Dissertation presented in fulfillment of the requirements for the Degree of Doctor of Philosophy in Biology (Molecular Genetics) at the Instituto de Tecnologia Qu��mica e Biol��gica da Universidade Nova de Lisboa

Recommending media content based on machine learning methods

Disserta����o para obten����o do Grau de Mestre em Engenharia Inform��tica

The clothing industry as a globalized sector: implications for work organisation, quality of work and job content

The clothing sector in several countries is still seen, in many aspects as a traditional sector with some average characteristics, nevertheless is a very important sector in terms of labour market. Globalization and de-localization are having a strong impact in the organisation of work and in occupational careers. Very few companies are able to keep a position in the market without changes in organisation of work and workers, founding different ways to face this reality according to size, capital and position. We could find two main paths: one where companies outsource production to another territory, close and/ or dismissal the workers; other path, where companies up skilled their capacities. This paper will present some results from the European project WORKS ��� Work organisation and restructuring in the knowledge society (6th Framework Programme), focusing the Portuguese case studies in several clothing companies in a comparative analysis with some other European countrie

An��lise da estrutura e da fun����o da prote��na morfogen��tica RodZ de Bacillus subtilis

Resumo: RodZ �� um componente do sistema morfogen��tico das c��lulas bacterianas. �� uma prote��na transmembranar que localiza em bandas ao longo do eixo longitudinal da c��lula. Em Bacillus subtilis, RodZ consiste numa por����o citoplasm��tica, RodZn, e em uma parte extra-citoplasm��tica, RodZc. RodZn cont��m um dom��nio em helixturn- helix (HTH), enquanto que RodZc pode ser dividido num dom��nio coiled-coil e num dom��nio terminal C, de fun����o desconhecida. Um segmento transmembranar (TM) ��nico separa RodZn de RodZc. A elimina����o de rodZ causa alongamento do nucle��ide e leva �� produ����o de c��lulas polares nucleadas. Aqui, mostramos que RodZn �� estruturado, est��vel e em h��lice ��. Descobrimos que as substitui����es Y32A e L33A na suposta h��lice de reconhecimento (���3) do motivo HTH, bem como as substitui����es Y49A e F53A, fora do motivo HTH (���4), causam divis��o assim��trica, mas apenas as ��ltimas levam �� deslocaliza����o sub-celular de RodZ. Sugerimos que as h��lices ���3 e ���4 s��o utilizadas para uma interac����o prote��na-prote��na ou prote��na- DNA essencial para divis��o celular enquanto que ���4 deve contactar um componente do citosqueleto, possivelmente MreB, uma vez que a correcta localiza����o sub-celular de RodZ depende desta prote��na. Em todos os mutantes as c��lulas polares s��o anucleadas, pelo que conclu��mos que o alongamento do nucle��ide n��o �� um pr��requisito para divis��o assim��trica. RodZc �� largamente n��o estruturado mas com conte��do de folha ���, sendo estabilizado pelo dom��nio coiled-coil. Mostramos uma rela����o hom��loga entre RodZc e a bomba de transporte Na+/Ca2+ NCX1 e identific��mos dois res��duos no dom��nio C, G265 e N275, essenciais para a manuten����o da forma celular. Estes res��duos fazem parte de um motivo em gancho que pode actuar como um local de interac����o com um ligando desconhecido. RodZn e RodZc s��o monom��ricos em solu����o. Contudo, na membrana, RodZ interage consigo pr��pria num sistema de dois h��bridos (Split-Ubiquitin) em levedura, sugerindo que possa formar mult��meros in vivo.-----------ABSTRACT: RodZ is a transmembrane component of the bacterial core morphogenic apparatus. RodZ localizes in bands long the longitudinal axis of the cell, and it is though to functionally link the cell wall to the actin cytoskeleton. In Bacillus subtilis, RodZ consists of a cytoplasmic moiety, RodZn, and an extracytoplasmic moiety, RodZc. RodZn contains a predicted helix-turn-helix domain, whereas RodZc is thought to contain a coiled-coil region and a terminal C domain of unknown function. A single transmembrane domain separates RodZn from RodZc. Deletion of rodZ causes elongation of the nucleoid and leads to the production of polar minicells containing DNA. Here, we have studied the structure and function of RodZn and RodZc. We show that RodZn is a stable, folded, ���-helical domain. We discovered that the Y32A and L33A substitutions within the presumptive recognition helix (���3) of the HTH motif, as well as the Y49A and F53A substitutions outside of the HTH motif (in ���4) cause asymmetric cell division. However, only the substitutions in ���4 cause sub-celular delocalization of RodZ. We suggest that ���3 and ���4 are used for a protein-protein or protein-DNA interaction important for cell division, whereas ���4 is likely to contact a cytoskeletal component, presumably MreB. The polar cells formed by all the mutants are anucleate. We conclude that nucleoid elongation is not a prerequisite for asymmetric division. RodZc appears to be a largely unstructured domain, with some ���-sheet content, and is stabilized by the coiled-coil region. We show a homology relationship between RodZc and the NCX1 Na+/Ca2+ transporter and we found two residues within the C domain, G265 and N275, that are important for cell shape determination. These residues are predicted to be essential determinants of a claw-like motif, which may act as a binding site for an unknown ligand. Both the isolated RodZn and RodZc proteins are monomeric in solution. However, because full-length RodZ interacts with itself in a split-ubiquitin yeast two-hybrid assay, we suggest that it may dimerize or form higher order multimers in vivo.

Host-induced changes in the cell surface N-linked glycoproteins, from Aspergillus fumigatus. Search for specific targets with potential for clinical therapy and/or diagnosis.

This dissertation is presented to obtain a Master degree in Structural and Functional Biochemistry

Improving search engines with open Web-based SKOS vocabularies

Disserta����o para obten����o do Grau de Mestre em Engenharia Inform��tica

Facing unemployment after being at the top: impact, job search strategies and crucial competences

A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA ��� School of Business and Economics