70 resultados para Software-based techniques


Relevância:

80.00% 80.00%

Publicador:

Resumo:

Trabalho apresentado no âmbito do European Master in Computational Logics, como requisito parcial para obtenção do grau de Mestre em Computational Logics

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Dissertation submitted to Faculdade de Ciências e Tecnologia - Universidade Nova de Lisboa in fulfilment of the requirements for the degree of Doctor of Philosophy (Biochemistry - Biotechnology)

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Staphylococcus aureus is an important opportunistic pathogen that can cause a wide variety of diseases from mild to life-threatening conditions. S. aureus can colonize many parts of the human body but the anterior nares are the primary ecological niche. Its clinical importance is due to its ability to resist almost all classes of antibiotics available together with its large number of virulence factores. MRSA (Methicillin-Resistant S. aureus) strains are particularly important in the hospital settings, being the major cause of nosocomial infections worldwide. MRSA resistance to β-lactam antibiotics involves the acquisition of the exogenous mecA gene, part of the SCCmec cassette. Fast and reliable diagnostic techniques are needed to reduce the mortality and morbidity associated with MRSA infections, through the early identification of MRSA strains. The current identification techniques are time-consuming as they usually involves culturing steps, taking up to five days to determine the antibiotic resistance profile. Several amplification-based techniques have been developed to accelerate the diagnosis. The aim of this project was to develop an even faster methodology that bypasses the DNA amplification step. Gold-nanoprobes were developed and used to detect the presence of mecA gene in S. aureus genome, associated with resistance traits, for colorimetric assays based on non-crosslinking method. Our results showed that the mecA and mecA_V2 gold-nanoprobes were sensitive enough to discriminate the presence of mecA gene in PCR products and genomic DNA (gDNA) samples for target concentrations of 10 ng/μL and 20 ng/μL, respectively. As our main objective was to avoid the amplification step, we concluded that the best strategy for the early identification of MRSA infection relies on colorimetric assays based on non-crosslinking method with gDNA samples that can be extracted directly from blood samples.

Relevância:

50.00% 50.00%

Publicador:

Resumo:

Dissertação apresentada para a obtenção do Grau de Doutor em Informática pela Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia

Relevância:

40.00% 40.00%

Publicador:

Resumo:

Trabalho apresentado no âmbito do Mestrado em Engenharia Informática, como requisito parcial para obtenção do grau de Mestre em Engenharia Informática

Relevância:

40.00% 40.00%

Publicador:

Resumo:

Dissertation presented to obtain the degree of Doctor of Philosophy in Electrical Engineering, speciality on Perceptional Systems, by the Universidade Nova de Lisboa, Faculty of Sciences and Technology

Relevância:

40.00% 40.00%

Publicador:

Resumo:

Thesis submitted to the Universidade Nova de Lisboa, Faculdade de Ciências e Tecnologia, for the degree of Doctor of Philosophy in Biochemistry

Relevância:

40.00% 40.00%

Publicador:

Resumo:

work presented in the context of the European Master’s program in Computational Logic, as the partial requirement for obtaining Master of Science degree in Computational Logic

Relevância:

40.00% 40.00%

Publicador:

Resumo:

Dissertação para obtenção do Grau de Doutor em Engenharia Informática

Relevância:

40.00% 40.00%

Publicador:

Resumo:

Dissertação para obtenção do Grau de Mestre em Engenharia Informática

Relevância:

30.00% 30.00%

Publicador:

Resumo:

In the past years, Software Architecture has attracted increased attention by academia and industry as the unifying concept to structure the design of complex systems. One particular research area deals with the possibility of reconfiguring architectures to adapt the systems they describe to new requirements. Reconfiguration amounts to adding and removing components and connections, and may have to occur without stopping the execution of the system being reconfigured. This work contributes to the formal description of such a process. Taking as a premise that a single formalism hardly ever satisfies all requirements in every situation, we present three approaches, each one with its own assumptions about the systems it can be applied to and with different advantages and disadvantages. Each approach is based on work of other researchers and has the aesthetic concern of changing as little as possible the original formalism, keeping its spirit. The first approach shows how a given reconfiguration can be specified in the same manner as the system it is applied to and in a way to be efficiently executed. The second approach explores the Chemical Abstract Machine, a formalism for rewriting multisets of terms, to describe architectures, computations, and reconfigurations in a uniform way. The last approach uses a UNITY-like parallel programming design language to describe computations, represents architectures by diagrams in the sense of Category Theory, and specifies reconfigurations by graph transformation rules.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

The aim of the TeleRisk Project on labour relations and professional risks within the context of teleworking in Portugal – supported by IDICT – Institute for Development and Inspection of Working Conditions (Ministry of Labour), is to study the practices and forms of teleworking in the manufacturing sectors in Portugal. The project chose also the software industry as a reference sector, even though it does not intend to exclude from the study any other sector of activity or the so-called “hybrid” forms of work. However, the latter must have some of the characteristics of telework. The project thus takes into account the so-called “traditional” sectors of activity, namely textile and machinery and metal engineering (machinery and equipment), not usually associated to this type of work. However, telework could include, in the so-called “traditional” sectors, other variations that are not found in technologically based sectors. One of the evaluation methods for the dynamics associated to telework consisted in carrying out surveys by means of questionnaires, aimed at employers in the sectors analysed. This paper presents some of the results of those surveys. It is important to mention that, being a preliminary analysis, it means that it does not pretend to have exhausted all the issues in the survey, but has meant that it shows the bigger tendencies, in terms of teleworking practices, of the Portuguese industry.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para a obtenção do Grau de Mestre em Engenharia Informática.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

Dissertação apresentada na Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa para a obtenção do grau de Mestre em Engenharia Informática.

Relevância:

30.00% 30.00%

Publicador:

Resumo:

Dissertação de Mestrado em Engenharia Informática