Superoxide reductase from the syphilis spirochete Treponema pallidum

Superoxide reductase is a 14 kDa metalloprotein containing a catalytic nonhaem iron centre [Fe(His)4Cys]. It is involved in defence mechanisms against oxygen toxicity, scavenging superoxide radicals from the cell. The oxidized form of Treponema pallidum superoxide reductase was crystallized in the presence of polyethylene glycol and magnesium chloride. Two crystal forms were obtained depending on the oxidizing agents used after purification: crystals grown in the presence of K3Fe(CN)6 belonged to space group P21 (unit-cell parameters a = 60.3, b = 59.9, c = 64.8 A ° , = 106.9 ) and diffracted beyond 1.60 A ° resolution, while crystals grown in the presence of Na2IrCl6 belonged to space group C2 (a = 119.4, b = 60.1, c = 65.6 A ° , = 104.9 ) and diffracted beyond 1.55 A ° . A highly redundant X-ray diffraction data set from the C2 crystal form collected on a copper rotating-anode generator ( = 1.542 A ° ) clearly defined the positions of the four Fe atoms present in the asymmetric unit by SAD methods. A MAD experiment at the iron absorption edge confirmed the positions of the previously determined iron sites and provided better phases for model building and refinement. Molecular replacement using the P21 data set was successful using a preliminary trace as a search model. A similar arrangement of the four protein molecules could be observed.

Synechocystis ferredoxin/ferredoxin-NADP+-reductase/NADP+ complex: Structural model obtained by NMR-restrained docking

FEBS Letters 579 (2005) 4585–4590

Le retable majeur de la Sé velha de Coimbra et la polychromie dans le diocèse de Coimbra à l’époque baroque: aspects techniques et esthétiques

Doutoramento em Conservação e Restauro, especialidade Teoria, História e Técnicas

Testemunhos funerários da Ilha de Marajó no Museu Dr. Santos Rocha e no Museu Nacional de Etnologia. Interpretação arqueológica

Dissertação de Mestrado em Arqueologia

De saussure as teoriasenunciativas ruptura ou continuidade?

pp. 49-59

Falar, falar,de que agora? Tudo acabou. Le cas sogntal (1974-1977)

«Lors de cette étude, nous avons interrogo et écouté les habitants du lieu. (-) Nous les avons tous rencontrés et avec presque tous, nous avons eu de longs et múltiples entretiens. Sollicitant T Information, provoquant les remémorations, laissant s'égrener les souvenirs..., nous avons cru recueillir les faits et, peu à peu, s'est imposée une parole: une vision du monde, une manière collective et originale de Ia dire et de Texpliquer.» Dans ces mots d'introduction à son livre La mêmoire longue, Françoise Zonabend met d'emblée Taccent sur Ia parole comme substrat de Ia mêmoire d'une communauté: parole individuelle recueillie au cours des entretiens menés auprès des informateurs, parole «collective» reconstituée par Tanthropologue après-coup et par écrit. Pour acquérir validité scientifique, les récits de vie recueillis auront été entretemps düment contextualisés grâce à d'autres sources d'Information, comme celles tirées de Tobservation ethnographique, des archives, etc.

The first crystal structure of class III superoxide reductase from Treponema pallidum

J Biol Inorg Chem (2006) 11: 548–558 DOI 10.1007/s00775-006-0104-y

Kinetics studies of the superoxide-mediated electron transfer reactions between rubredoxin-type proteins and superoxide reductases

J Biol Inorg Chem (2006) 11: 433–444 DOI 10.1007/s00775-006-0090-0

Overexpression and purification of Treponema pallidum rubredoxin; kinetic evidence for a superoxide-mediated electron transfer with the superoxide reductase neelaredoxin

J Biol Inorg Chem (2004) 9: 839–849 DOI 10.1007/s00775-004-0584-6

Formation of a stable cyano-bridged dinuclear iron cluster following oxidation of the superoxide reductases from Treponema pallidum and Desulfovibrio vulgaris with K(3)Fe(CN)(6)

Inorg. Chem., 2003, 42 (4), pp 938–940 DOI: 10.1021/ic0262886

Analysis of the progress of lifelong learning strategies of Portuguese Economics and Management Schools

A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA – School of Business and Economics

Apoio à implementação do Sistema de Informação Territorial da Administração Interna e à análise de padrões espaciais

Este documento visa apresentar o trabalho de Estágio realizado na Direção Geral de Administração Interna (DGAI) como parte integrante e conclusiva do Mestrado em Gestão do Território na Área de Especialização em Deteção Remota e Sistemas de Informação Geográfica, ministrado pela Faculdade de Ciências Sociais e Humanas da Universidade Nova de Lisboa. O Estágio desenvolveu-se em duas fases. A primeira detém um caráter mais genérico e teve como objetivo prestar apoio à consolidação do Sistema de Informação Territorial da Administração Interna (SITAI), onde, entre diversas atividades desenvolvidas, a principal, e conforme exposto no presente relatório, reside na adaptação de dados do Instituto Nacional de Estatística (Censos 2011) ao território nacional após a Reorganização Administrativa do Território das Freguesias (RATF). A segunda enfoca o fenómeno da Violência Doméstica (VD) e consiste no tratamento e análise de vários tipos de dados provenientes da base de dados do Ministério da Administração Interna (MAI) relacionados com o mesmo. Não obstante esta segmentação de trabalhos, é objetivo deste Estágio a aplicação dos Sistemas de Informação Geográfica (SIG) envolvendo dados de realidades específicas e a produção de informação capaz de se constituir como instrumento de suporte à decisão e desenvolvimento de políticas de segurança interna pelas quais o MAI é responsável.

How to improve urban mobility in Lisbon: intermodality and information and ticketing systems

Urban mobility is one of the main challenges facing urban areas due to the growing population and to traffic congestion, resulting in environmental pressures. The pathway to urban sustainable mobility involves strengthening of intermodal mobility. The integrated use of different transport modes is getting more and more important and intermodality has been mentioned as a way for public transport compete with private cars. The aim of the current dissertation is to define a set of strategies to improve urban mobility in Lisbon and by consequence reduce the environmental impacts of transports. In order to do that several intermodal practices over Europe were analysed and the transport systems of Brussels and Lisbon were studied and compared, giving special attention to intermodal systems. In the case study was gathered data from both cities in the field, by using and observing the different transport modes, and two surveys were done to the cities users. As concluded by the study, Brussels and Lisbon present significant differences. In Brussels the measures to promote intermodality are evident, while in Lisbon a lot still needs to be done. It also made clear the necessity for improvements in Lisbon’s public transports to a more intermodal passenger transport system, through integration of different transport modes and better information and ticketing system. Some of the points requiring developments are: interchanges’ waiting areas; integration of bicycle in public transport; information about correspondences with other transport modes; real-time information to passengers pre-trip and on-trip, especially in buses and trams. After the identification of the best practices in Brussels and the weaknesses in Lisbon the possibility of applying some of the practices in Brussels to Lisbon was evaluated. Brussels demonstrated to be a good example of intermodality and for that reason some of the recommendations to improve intermodal mobility in Lisbon can follow the practices in place in Brussels.