Fabrication and characterization of transparent conductive oxide surface sensors for electrocorticography – TrECoG

Understanding how the brain works has been one of the greatest goals of mankind. This desire fuels the scientific community to pursue novel techniques able to acquire the complex information produced by the brain at any given moment. The Electrocorticography (ECoG) is one of those techniques. By placing conductive electrodes over the dura, or directly over the cortex, and measuring the electric potential variation, one can acquire information regarding the activation of those areas. In this work, transparent ECoGs, (TrECoGs) are fabricated through thin film deposition of the Transparent Conductive Oxides (TCOs) Indium-Zinc-Oxide (IZO) and Gallium-Zinc-Oxide (GZO). Five distinct devices have been fabricated via shadow masking and photolithography. The data acquired and presented in this work validates the TrECoGs fabricated as efficient devices for recording brain activity. The best results were obtained for the GZO- based TrECoG, which presented an average impedance of 36 kΩ at 1 kHz for 500 μm diameter electrodes, a transmittance close to 90% for the visible spectrum and a clear capability to detect brain signal variations. The IZO based devices also presented high transmittance levels (90%), but with higher impedances, which ranged from 40 kΩ to 100 kΩ.

Study of in vivo interactions between penicillin-binding proteins of Staphylococcus aureus

Staphylococcus aureus (S. aureus) is a major human pathogen that has acquired resistance to practically all classes of β-lactam antibiotics, being responsible of Multidrug resistant S. aureus (MRSA) associated infections both in healthcare (HA-MRSA) and community settings (CA-MRSA). The emergence of laboratory strains with high-resistance (VRSA) to the last resort antibiotic, vancomycin, is a warning of what is to come in clinical strains. Penicillin binding proteins (PBPs) target β-lactams and are responsible for catalyzing the last steps of synthesis of the main component of cell wall, peptidoglycan. As in Escherichia coli, it is suggested that S. aureus uses a multi-protein complex that carries out cell wall synthesis. In the presence of β-lactams, PBP2A and PBP2 perform a joint action to build the cell wall and allow cell survival. Likewise, PBP2 cooperates with PBP4 in cell wall cross-linking. However, an actual interaction between PBP2 and PBP4 and the location of such interaction has not yet been determined. Therefore, investigation of the existence of a PBP2-PBP4 interaction and its location(s) in vivo is of great interest, as it should provide new insights into the function of the cell wall synthesis machinery in S. aureus. The aim of this work was to develop Split-GFPP7 system to determine interactions between PBP2 and PBP4. GFPP7 was split in a strategic site and fused to proteins of interest. When each GFPP7 fragment, fused to proteins, was expressed alone in staphylococcal cells, no fluorescence was detectable. When GFPP7 fragments fused to different peptidoglycan synthesis (PBP2 and PBP4) or cell division (FtsZ and EzrA) proteins were co-expressed together, fluorescent fusions were localized to the septum. However, further analysis revealed that this positive result is mediated by GFPP7 self-association. We then interpret the results in light of such event and provide insights into ways of improving this system.

Plataforma de gestão educativa de mediação entre a secretaria e a comunidade educativa

O presente Relatório tem como objetivo expor o trabalho desenvolvido durante o estágio, no âmbito da componente não letiva do mestrado em Ciências da Educação, realizado ao abrigo de um protocolo entre a Faculdade de Ciências Socais e Humanas (FCSH) da Universidade Nova de Lisboa (UNL) e a Escola Profissional Gustave Eiffel (EPGE), sob orientação científica do Professor Doutor Luís Manuel Bernardo da FCSH e orientação prática pela Mestre Maria Goreti Freitas, da EPGE. O estágio teve a duração de três meses, com um total de 400 horas. Deste modo, serão descritas as práticas de estágio, sustentadas nos conhecimentos adquiridos nas componentes letivas do mestrado em Ciências da Educação. O relatório está estruturado em três capítulos, finalizando com uma reflexão sobre a produtividade do estágio para a nossa aprendizagem. No primeiro capítulo, pretende-se justificar a escolha da temática deste relatório, procedendo-se à caracterização da secretaria e da escola, fundamentada através de uma revisão de literatura, bem como por algumas entrevistas realizadas durante o estágio. Neste capítulo dá-se importância ao facto deste estágio ter permitido uma interação com toda a comunidade educativa, nas suas respetivas diferenças, em diferentes níveis e situações. No segundo capítulo, efetuou-se a uma contextualização acerca das plataformas de gestão educativas bem como a sua importância para a gestão da comunidade educativa. Evidenciou-se em particular o papel das plataformas de gestão na educação, enquanto elo de ligação entre a secretaria e toda a comunidade educativa. No terceiro capítulo levou-se a cabo a descrição das principais atividades desenvolvidas durante o estágio curricular. Por fim, apresenta-se a uma reflexão crítica sobre o processo de estágio. Todo o trabalho remete para documentos anexos, os quais comprovam a documentação complementar. Alguns destes documentos foram elaborados de forma original, designadamente as entrevistas realizadas no decurso do estágio.

Community indentification in peer-t-peer collaborative consumption services

Even though collaborative consumption (CC) is gaining economic importance, research in CC is still in its infancy. Consumers’ reasons for participating have already been investigated but little research on consequences of participation has been conducted. This article examines whether interactions between customers in peer-to-peer CC services influence the willingness to coproduce service outcomes. Drawing on social exchange theory, it is proposed that this effect is mediated by consumers’ identification with the brand community. Furthermore, continuance intention in CC is introduced as a second stage moderator. In a cross-sectional study, customers of peer-to-peer accommodation sharing are surveyed. While customer-to-customer interactions were found to have a positive effect on brand community identification, brand community identification did not positively affect co-production intention. Surprisingly, the effect of brand community identification on co-production intention was negative. Moreover, continuance intention of customers did not moderate this relationship. Bearing in mind current challenges for researchers and companies, theoretical and managerial implications are discussed.