46 resultados para Target Held Method
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Dissertation to obtain a Master’s Degree in Chemical and Biochemical Engineering
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Dissertação para obtenção do Grau de Mestre em Biotecnologia
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Based on the report for the “Project III” unit of the PhD programme on Technology Assessment under the supervision of Prof. António B. Moniz. This report was discussed also at the 2nd Winter School on Technology Assessment held at Universidade Nova de Lisboa, Caparica Campus, Portugal on December 2011.
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Dissertation presented to obtain the Ph.D degree in Molecular Biology
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Dissertation submitted in partial fulfillment of the requirements for the Degree of Master of Science in Geospatial Technologies.
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Dissertation to obtain the Master Degree in Biotechnology
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A Work Project, presented as part of the requirements for the Award of a Masters Degree in Finance from the NOVA – School of Business and Economics
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A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA – School of Business and Economics
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A Work Project, presented as part of the requirements for the Award of a Masters Degree in Management from the NOVA – School of Business and Economics
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RESUMO - O processo de comunicação em saúde encontra-se relacionado com a melhoria dos resultados em saúde através da informação, com o objectivo de influenciar indivíduos e comunidades. Os profissionais de saúde, no contexto da prestação de cuidados, necessitam de deter determinadas competências interpessoais e de comunicação no sentido de promover a qualidade e segurança do doente, contribuindo para a diminuição do erro clínico através de uma comunicação eficaz em equipa. Estabelecer uma relação de empatia e confiança com o doente, saber escutar e compreender a sua perspectiva definem-se, entre outras, por capacidades essenciais a estes profissionais, a par de uma comunicação verbal e escrita clara e assertiva, cujo impacto se reflecte também na satisfação do utente. O ensino destas competências é fundamental para habilitar os profissionais a lidar com diversas situações, contribuindo para a excelência técnica e qualidade dos cuidados. A sua avaliação é essencialmente realizada durante a formação académica, a nível internacional, sobre o que a literatura descreve várias metodologias possíveis. O método utilizado com maior frequência consiste na observação directa da interacção entre o aluno e o doente, em contexto real ou simulado, através de uma checklist formada por itens correspondentes a atitudes e comportamentos que avalia a presença das competências necessárias. O desenvolvimento destas competências passa por sensibilizar os profissionais para a sua importância, dar-lhes formação e avaliar, apostando na continuidade da investigação.
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A Work Project, presented as part of the requirements for the Award of a Masters Degree in Finance from the NOVA – School of Business and Economics
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Dissertação para obtenção do Grau de Mestre em Engenharia Electrotécnica e de Computadores
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Dissertation to obtain the degree of Doctor of Philosophy in Biomedical Engineering
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Dissertation presented to obtain the Ph.D degree in Chemistry.
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This work aimed at the development of a (bio)polymeric monolithic support for biopharmaceuticals purification and/or capture. For that, it was assured that functional groups on its surface were ready to be involved in a plethora of chemical reactions for incorporation of the desired and most suitable ligand. Using cryogelation as preparation method a screening on multiple combinations of materials was performed in order to create a potentially efficient support with the minimal footprint, i.e. a monolithic support with reasonable mechanical properties, highly permeable, biocompatible, ready to use, with gravitational performance and minimal unspecific interactions towards the target molecules, but also biodegradable and produced from renewable materials. For the pre-selection all monoliths were characterized physico-chemically and morphologically; one agarose-based and two chitosan-based monoliths were then subjected to further characterizations before and after their modification with magnetic nanoparticles. These three specimens were finally tested towards adenovirus and the recovery reached 84% for the chitosan-GMA plain monolith prepared at -80°C. Monoliths based on chitosan and PVA were prepared in the presence and absence of magnetic particles, and tested for the isolation of GFP directly from crude cellular extracts. The affinity ligand A4C7 previously selected for GFP purification was synthesized on the monolith. The results indicated that the solid-phase synthesis of the ligand directly onto the monolith might require optimization and that the large pores of the monoliths are unsuitable for the purification of small proteins, such as GFP.