Cytotoxicity in L929 fibroblasts and inhibition of herpes simplex virus type 1 Kupka by estuarine cyanobacteria extracts

The cyanobacteria are known to be a rich source of metabolites with a variety of biological activities in different biological systems. In the present work, the bioactivity of aqueous and organic (methanolic and hexane) crude extracts of cyanobacteria isolated from estuarine ecosystems was studied using different bioassays. The assessment of DNA damage on the SOS gene repair region of mutant PQ37 strain of Escherichia coli was performed. Antiviral activity was evaluated against influenza virus, HRV-2, CVB3 and HSV-1 viruses using crystal violet dye uptake on HeLa, MDCK and GMK cell lines. Cytotoxicity evaluation was performed with L929 fibroblasts by MTT assay. Of a total of 18 cyanobacterial isolates studied, only the crude methanolic extract of LEGE 06078 proved to be genotoxic (IF > 1.5) in a dose-dependent manner and other four were putative candidates to induce DNA damage. Furthermore, the crude aqueous extract of LEGE 07085 showed anti- herpes type 1 activity (IC50 = 174.10 μg dry extract mL−1) while not presenting any cytotoxic activity against GMK cell lines. Of the 54 cyanobacterial extracts tested, only the crude methanolic and hexane ones showed impair on metabolic activity of L929 fibroblasts after long exposure (48–72 h). The inhibition of HSV-1 and the strong cytotoxicity against L929 cells observed emphasizes the importance of evaluating the impact of those estuarine cyanobacteria on aquatic ecosystem and on human health. The data also point out their potential application in HSV-1 treatment and pharmacological interest.

Provably good scheduling of sporadic tasks with resource sharing on a two-type heterogeneous multiprocessor platform

Consider the problem of scheduling a set of implicit-deadline sporadic tasks to meet all deadlines on a two-type heterogeneous multiprocessor platform where a task may request at most one of |R| shared resources. There are m1 processors of type-1 and m2 processors of type-2. Tasks may migrate only when requesting or releasing resources. We present a new algorithm, FF-3C-vpr, which offers a guarantee that if a task set is schedulable to meet deadlines by an optimal task assignment scheme that only allows tasks to migrate when requesting or releasing a resource, then FF-3Cvpr also meets deadlines if given processors 4+6*ceil(|R|/min(m1,m2)) times as fast. As far as we know, it is the first result for resource sharing on heterogeneous platforms with provable performance.

Intra-type migrative scheduling of implicit-deadline sporadic tasks on two- type heterogeneous multiprocessor

Consider the problem of scheduling a set of implicit-deadline sporadic tasks to meet all deadlines on a two-type heterogeneous multiprocessor platform. Each processor is either of type-1 or type-2 with each task having different execution time on each processor type. Jobs can migrate between processors of same type (referred to as intra-type migration) but cannot migrate between processors of different types. We present a new scheduling algorithm namely, LP-Relax(THR) which offers a guarantee that if a task set can be scheduled to meet deadlines by an optimal task assignment scheme that allows intra-type migration then LP-Relax(THR) meets deadlines as well with intra-type migration if given processors 1/THR as fast (referred to as speed competitive ratio) where THR <= 2/3.