Road drivers performance appraisal using a MCDA method: a case study in the road transport company

Performance evaluation increasingly assumes a more important role in any organizational environment. In the transport area, the drivers are the company’s image and for this reason it is important to develop and increase their performance and commitment to the company goals. This evaluation can be used to motivate driver to improve their performance and to discover training needs. This work aims to create a performance appraisal evaluation model of the drivers based on the multi-criteria decision aid methodology. The MMASSI (Multicriteria Methodology to Support Selection of Information Systems) methodology was adapted by using a template supporting the evaluation according to the freight transportation company in study. The evaluation process involved all drivers (collaborators being evaluated), their supervisors and the company management. The final output is a ranking of the drivers, based on their performance, for each one of the scenarios used.

The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies

On solid substrates, yeast colonies pass through distinct developmental phases characterized by the changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum-sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes, among others, the activation of several plasma membrane transporters and is connected with colony differentiation. In the present study, we show that colony cells can use two transport mechanisms to import lactic acid: a ‘saturable’ component of the transport, which requires the presence of a functional Jen1p transporter, and a ‘non-saturable’ component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. Although the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the second acidic phase. This lactate uptake profile correlates with the localization of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of non-saturable lactic acid uptake in yeast colonies.

Specific label-free and real-time detection of oxidized low density lipoprotein (oxLDL) using an immunosensor with three monoclonal antibodies

Increased levels of plasma oxLDL, which is the oxidized fraction of Low Density Lipoprotein (LDL), are associated with atherosclerosis, an inflammatory disease, and the subsequent development of severe cardiovascular diseases that are today a major cause of death in modern countries. It is therefore important to find a reliable and fast assay to determine oxLDL in serum. A new immunosensor employing three monoclonal antibodies (mAbs) against oxLDL is proposed in this work as a quick and effective way to monitor oxLDL. The oxLDL was first employed to produce anti-oxLDL monoclonal antibodies by hybridoma cells that were previously obtained. The immunosensor was set-up by selfassembling cysteamine (Cyst) on a gold (Au) layer (4 mm diameter) of a disposable screen-printed electrode. Three mAbs were allowed to react with N-hydroxysuccinimide (NHS) and ethyl(dimethylaminopropyl)carbodiimide (EDAC), and subsequently incubated in the Au/Cys. Albumin from bovine serum (BSA) was immobilized further to ensure that other molecules apart from oxLDL could not bind to the electrode surface. All steps were followed by various characterization techniques such as electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV). The analytical operation of the immunosensor was obtained by incubating the sensing layer of the device in oxLDL for 15 minutes, prior to EIS and SWV. This was done by using standard oxLDL solutions prepared in foetal calf serum, in order to simulate patient's plasma with circulating oxLDL. A sensitive response was observed from 0.5 to 18.0 mg mL 1 . The device was successfully applied to determine the oxLDL fraction in real serum, without prior dilution or necessary chemical treatment. The use of multiple monoclonal antibodies on a biosensing platform seemed to be a successful approach to produce a specific response towards a complex multi-analyte target, correlating well with the level of oxLDL within atherosclerosis disease, in a simple, fast and cheap way.

Effects of Soil Compaction and Organic Carbon Content on Preferential Flow in Loamy Field Soils

Abstract: Preferential flow and transport through macropores affect plant water use efficiency and enhance leaching of agrochemicals and the transport of colloids, thereby increasing the risk for contamination of groundwater resources. The effects of soil compaction, expressed in terms of bulk density (BD), and organic carbon (OC) content on preferential flow and transport were investigated using 150 undisturbed soil cores sampled from 15 × 15–m grids on two field sites. Both fields had loamy textures, but one site had significantly higher OC content. Leaching experiments were conducted in each core by applying a constant irrigation rate of 10 mm h−1 with a pulse application of tritium tracer. Five percent tritium mass arrival times and apparent dispersivities were derived from each of the tracer breakthrough curves and correlated with texture, OC content, and BD to assess the spatial distribution of preferential flow and transport across the investigated fields. Soils from both fields showed strong positive correlations between BD and preferential flow. Interestingly, the relationships between BD and tracer transport characteristics were markedly different for the two fields, although the relationship between BD and macroporosity was nearly identical. The difference was likely caused by the higher contents of fines and OC at one of the fields leading to stronger aggregation, smaller matrix permeability, and a more pronounced pipe-like pore system with well-aligned macropores.

Predictivity Strength of the Spatial Variability of Phenanthrene Sorption Across Two Sandy Loam Fields

Sorption is commonly agreed to be the major process underlying the transport and fate of polycyclic aromatic hydrocarbons (PAHs) in soils. However, there is still a scarcity of studies focusing on spatial variability at the field scale in particular. In order to investigate the variation in the field of phenanthrene sorption, bulk topsoil samples were taken in a 15 × 15-m grid from the plough layer in two sandy loam fields with different texture and organic carbon (OC) contents (140 samples in total). Batch experiments were performed using the adsorption method. Values for the partition coefficient K d (L kg−1) and the organic carbon partition coefficient K OC (L kg−1) agreed with the most frequently used models for PAH partitioning, as OC revealed a higher affinity for sorption. More complex models using different OC compartments, such as non-complexed organic carbon (NCOC) and complexed organic carbon (COC) separately, performed better than single K OC models, particularly for a subset including samples with Dexter n < 10 and OC <0.04 kg kg−1. The selected threshold revealed that K OC-based models proved to be applicable for more organic fields, while two-component models proved to be more accurate for the prediction of K d and retardation factor (R) for less organic soils. Moreover, OC did not fully reflect the changes in phenanthrene retardation in the field with lower OC content (Faardrup). Bulk density and available water content influenced the phenanthrene transport mechanism phenomenon.

Longshore Transport Estimation on Ofir Beach in Northwest Portugal: Sand-Tracer Experiment

This work aims to shed some light on longshore sediment transport (LST) in the highly energetic northwest coast of Portugal. Data achieved through a sand-tracer experiment are compared with data obtained from the original and the new re-evaluated longshore sediment transport formulas (USACE Waterways Experiment Station’s Coastal Engineering and Research Center, Kamphuis, and Bayram bulk formulas) to assess their performance. The field experiment with dyed sand was held at Ofir Beach during one tidal cycle under medium wave-energy conditions. Local hydrodynamic conditions and beach topography were recorded. The tracer was driven southward in response to the local swell and wind- and wave-induced currents (Hsb=0.75mHsb=0.75m, Tp=11.5sTp=11.5s, θb=8−12°θb=8−12°). The LST was estimated by using a linear sediment transport flux approach. The obtained value (2.3×10−3m3⋅s−12.3×10−3m3⋅s−1) approached the estimation provided by the original Bayram formula (2.5×10−3m3⋅s−12.5×10−3m3⋅s−1). The other formulas overestimated the transport, but the estimations resulting from the new re-evaluated formulas also yield approximate results. Therefore, the results of this work indicated that the Bayram formula may give satisfactory results for predicting the longshore sediment transport on Ofir Beach.

Serviço de correio eletrónico em ambiente marítimo utilizando redes tolerantes ao atraso

Delay Tolerant Network (DTN) é uma arquitetura de redes que procura resolver os problemas associados à conetividade intermitente de sistemas e possibilita a existência de comunicações em ambientes onde o conjunto de protocolos tradicionais TCP/IP não funciona. A arquitetura DTN é adequada a cenários com uma topologia de rede dinâmica, densidade de nós reduzida, conetividade intermitente e de curta duração entre os nós, e em que as aplicações são tolerantes ao atraso. Nesta dissertação é apresentada uma solução de baixo custo recorrendo ao conceito DTN que permite a utilizadores de embarcações utilizarem o serviço de correio eletrónico no mar. A solução estende o sistema de correio eletrónico ao cenário marítimo recorrendo a estações na costa, comunicação sem fios entre embarcações e entre estas e a estações na costa, e à capacidade das embarcações funcionarem como meios de transporte de dados. Para proceder à validação da proposta apresentada, foi implementado um protótipo com o sistema de correio eletrónico adaptado ao cenário marítimo. O protótipo é constituído por vários nós, configurados de forma a assumir o papel de embarcações, estação da costa e um servidor de e-mail presente na Internet. Os resultados dos testes experimentais realizados em ambiente controlado mostram que os objetivos do trabalho foram alcançados. O serviço de e-mail assente sobre a arquitetura DTN e adaptado ao cenário de comunicações marítimo foi testado em diferentes contextos, e em todos eles, as experiências tiveram resultados positivos.