Optimizing potentiometric ionophore and electrode design for environmental on-site control of antibiotic drugs: Application to sulfamethoxazole

Potentiometric sensors are typically unable to carry out on-site monitoring of environmental drug contaminants because of their high limits of detection (LODs). Designing a novel ligand material for the target analyte and managing the composition of the internal reference solution have been the strategies employed here to produce for the first time a potentiometric-based direct reading method for an environmental drug contaminant. This concept has been applied to sulfamethoxazole (SMX), one of the many antibiotics used in aquaculture practices that may occur in environmental waters. The novel ligand has been produced by imprinting SMX on the surface of graphitic carbon nanostructures (CN) < 500 nm. The imprinted carbon nanostructures (ICN) were dispersed in plasticizer and entrapped in a PVC matrix that included (or not) a small amount of a lipophilic additive. The membrane composition was optimized on solid-contact electrodes, allowing near-Nernstian responses down to 5.2 μg/mL and detecting 1.6 μg/mL. The membranes offered good selectivity against most of the ionic compounds in environmental water. The best membrane cocktail was applied on the smaller end of a 1000 μL micropipette tip made of polypropylene. The tip was then filled with inner reference solution containing SMX and chlorate (as interfering compound). The corresponding concentrations were studied for 1 × 10−5 to 1 × 10−10 and 1 × 10−3 to 1 × 10−8 mol/L. The best condition allowed the detection of 5.92 ng/L (or 2.3 × 10−8 mol/L) SMX for a sub-Nernstian slope of −40.3 mV/decade from 5.0 × 10−8 to 2.4 × 10−5 mol/L.

Gestão e Direcção de Obra

Este relatório diz respeito ao trabalho desenvolvido em ambiente de estágio curricular na construção de um empreendimento comercial na zona de Oeiras, compreendendo a construção de um edifício, infra-estruturas enterradas e arranjos exteriores. O estágio foi realizado na Volare – Gestão de Projectos, S.A., empresa dedicada à execução de obras de construção civil destinadas ao grupo Ibersol. Embora todas as áreas de gestão abordadas no estudo sejam de extrema importância, as áreas de gestão do custo, gestão das compras e gestão do tempo revelam-se ainda extremamente valorizadas pelas empresas de construção – o que faz com continuem a existir derrapagens orçamentais e incumprimento de prazos. Só uma gestão rigorosa, eficiente e capaz de abraçar todas as áreas de conhecimentos abordadas, tornará possível alcançar os patamares de satisfação das empresas e dos clientes. O presente relatório mostra que o gestor de obras deve possuir grande capacidade de liderança, de comunicação e negociação O objectivo deste relatório, para além da descrição de todos os trabalhos práticos de execução da obra, é de transcrever os conhecimentos aplicados ao longo do estágio, nomeadamente no que concerne aos dos processos de ‘Gestão, Coordenação e Planeamento’, directamente ligados as funções da ‘Direcção Técnica de Obra’ durante toda a fase de execução da empreitada, estando o principal foco na descrição das actividades referentes ao controlo da obra.

Rapid automated method for on-site determination of sulfadiazine in fish farming: a stainless steel veterinary syringe coated with a selective membrane of PVC serving as a potentiometric detector in a flow-injection-analysis system

Sulfadiazine is an antibiotic of the sulfonamide group and is used as a veterinary drug in fish farming. Monitoring it in the tanks is fundamental to control the applied doses and avoid environmental dissemination. Pursuing this goal, we included a novel potentiometric design in a flow-injection assembly. The electrode body was a stainless steel needle veterinary syringe of 0.8-mm inner diameter. A selective membrane of PVC acted as a sensory surface. Its composition, the length of the electrode, and other flow variables were optimized. The best performance was obtained for sensors of 1.5-cm length and a membrane composition of 33% PVC, 66% onitrophenyloctyl ether, 1% ion exchanger, and a small amount of a cationic additive. It exhibited Nernstian slopes of 61.0 mV decade-1 down to 1.0×10-5 mol L-1, with a limit of detection of 3.1×10-6 mol L-1 in flowing media. All necessary pH/ionic strength adjustments were performed online by merging the sample plug with a buffer carrier of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, pH 4.9. The sensor exhibited the advantages of a fast response time (less than 15 s), long operational lifetime (60 days), and good selectivity for chloride, nitrite, acetate, tartrate, citrate, and ascorbate. The flow setup was successfully applied to the analysis of aquaculture waters. The analytical results were validated against those obtained with liquid chromatography–tandem mass spectrometry procedures. The sampling rate was about 84 samples per hour and recoveries ranged from 95.9 to 106.9%.