JamLab: augmenting sensornet testbeds with realistic and controlled interference generation

Radio interference drastically affects the performance of sensor-net communications, leading to packet loss and reduced energy-efficiency. As an increasing number of wireless devices operates on the same ISM frequencies, there is a strong need for understanding and debugging the performance of existing sensornet protocols under interference. Doing so requires a low-cost flexible testbed infrastructure that allows the repeatable generation of a wide range of interference patterns. Unfortunately, to date, existing sensornet testbeds lack such capabilities, and do not permit to study easily the coexistence problems between devices sharing the same frequencies. This paper addresses the current lack of such an infrastructure by using off-the-shelf sensor motes to record and playback interference patterns as well as to generate customizable and repeat-able interference in real-time. We propose and develop JamLab: a low-cost infrastructure to augment existing sensornet testbeds with accurate interference generation while limiting the overhead to a simple upload of the appropriate software. We explain how we tackle the hardware limitations and get an accurate measurement and regeneration of interference, and we experimentally evaluate the accuracy of JamLab with respect to time, space, and intensity. We further use JamLab to characterize the impact of interference on sensornet MAC protocols.

Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-Screen Printed Electrodes

A gold screen printed electrode (Au-SPE) was modified by merging Molecular Imprinting and Self-Assembly Monolayer techniques for fast screening cardiac biomarkers in point-of-care (POC). For this purpose, Myoglobin (Myo) was selected as target analyte and its plastic antibody imprinted over a glutaraldehyde (Glu)/cysteamine (Cys) layer on the gold-surface. The imprinting effect was produced by growing a reticulated polymer of acrylamide (AAM) and N,N′-methylenebisacrylamide (NNMBA) around the Myo template, covalently attached to the biosensing surface. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) studies were carried out in all chemical modification steps to confirm the surface changes in the Au-SPE. The analytical features of the resulting biosensor were studied by different electrochemical techniques, including EIS, square wave voltammetry (SWV) and potentiometry. The limits of detection ranged from 0.13 to 8 μg/mL. Only potentiometry assays showed limits of detection including the cut-off Myo levels. Quantitative information was also produced for Myo concentrations ≥0.2 μg/mL. The linear response of the biosensing device showed an anionic slope of ~70 mV per decade molar concentration up to 0.3 μg/mL. The interference of coexisting species was tested and good selectivity was observed. The biosensor was successfully applied to biological fluids.

Smart Plastic Antibody Material (SPAM) tailored on disposable screen printed electrodes for protein recognition: application to Myoglobin detection

This work introduces two major changes to the conventional protocol for designing plastic antibodies: (i) the imprinted sites were created with charged monomers while the surrounding environment was tailored using neutral material; and (ii) the protein was removed from its imprinted site by means of a protease, aiming at preserving the polymeric network of the plastic antibody. To our knowledge, these approaches were never presented before and the resulting material was named here as smart plastic antibody material (SPAM). As proof of concept, SPAM was tailored on top of disposable gold-screen printed electrodes (Au-SPE), following a bottom-up approach, for targeting myoglobin (Myo) in a point-of-care context. The existence of imprinted sites was checked by comparing a SPAM modified surface to a negative control, consisting of similar material where the template was omitted from the procedure and called non-imprinted materials (NIMs). All stages of the creation of the SPAM and NIM on the Au layer were followed by both electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). AFM imaging was also performed to characterize the topography of the surface. There are two major reasons supporting the fact that plastic antibodies were effectively designed by the above approach: (i) they were visualized for the first time by AFM, being present only in the SPAM network; and (ii) only the SPAM material was able to rebind to the target protein and produce a linear electrical response against EIS and square wave voltammetry (SWV) assays, with NIMs showing a similar-to-random behavior. The SPAM/Au-SPE devices displayed linear responses to Myo in EIS and SWV assays down to 3.5 μg/mL and 0.58 μg/mL, respectively, with detection limits of 1.5 and 0.28 μg/mL. SPAM materials also showed negligible interference from troponin T (TnT), bovine serum albumin (BSA) and urea under SWV assays, showing promising results for point-of-care applications when applied to spiked biological fluids.

Scalable and interference aware wi-fi mesh networks using cots devices

A crescente tendencia no acesso móvel tem sido potenciada pela tecnologia IEEE 802.11. Contudo, estas redes têm alcance rádio limitado. Para a extensão da sua cobertura é possível recorrer a redes emalhadas sem fios baseadas na tecnologia IEEE 802.11, com vantagem do ponto de vista do custo e da flexibilidade de instalação, face a soluções cabladas. Redes emalhadas sem fios constituídas por nós com apenas uma interface têm escalabilidade reduzida. A principal razão dessa limitação deve-se ao uso do mecanismo de acesso ao meio partilhado Carrier Sense Multiple Access with Collision Avoidance (CSMA/CA) em topologias multi-hop. Especificamente, o CSMA/CA não evita o problema do nó escondido levando ao aumento do número de colisões e correspondente degradação de desempenho com impacto direto no throughput e na latência. Com a redução da tecnologia rádio torna-se viável a utilização de múltiplos rádios por nó, sem com isso aumentar significativamente o custo da solução final de comunicações. A utilização de mais do que um rádio por nó de comuniações permite superar os problemas de desempenho inerentes ás redes formadas por nós com apenas um rádio. O objetivo desta tese, passa por desenvolver uma nova solução para redes emalhadas multi-cana, duar-radio, utilizando para isso novos mecanismos que complementam os mecanismos definidos no IEEE 802.11 para o estabelecimento de um Basic Service Set (BSS). A solução é baseada na solução WiFIX, um protocolo de routing para redes emalhadas de interface única e reutiliza os mecanismos já implementados nas redes IEEE 802.11 para difundir métricas que permitam à rede escalar de forma eficaz minimizando o impacto na performance. A rede multi-hop é formada por nós equipados com duas interfaces, organizados numa topologia hierárquica sobre múltiplas relações Access Point (AP) – Station (STA). Os resultados experimentais obtidos mostram a eficácia e o bom desempenho da solução proposta face à solução WiFIX original.