Extraction of hemoglobin with calixarenes and biocatalysis in organic media of the complex with pseudoactivity of peroxidase

The present work involves the use of p-tert-butylcalix[4,6,8]arene carboxylic acid derivatives ((t)Butyl[4,6,8]CH2COOH) for selective extraction of hemoglobin. All three calixarenes extracted hemoglobin into the organic phase, exhibiting extraction parameters higher than 0.90. Evaluation of the solvent accessible positively charged amino acid side chains of hemoglobin (PDB entry 1XZ2) revealed that there are 8 arginine, 44 lysine and 30 histidine residues on the protein surface which may be involved in the interactions with the calixarene molecules. The hemoglobin-(t)Butyl[6]CH2COOH complex had pseudoperoxidase activity which catalysed the oxidation of syringaldazine in the presence of hydrogen peroxide in organic medium containing chloroform. The effect of pH, protein and substrate concentrations on biocatalysis was investigated using the hemoglobin-(t)Butyl[6]CH2COOH complex. This complex exhibited the highest specific activity of 9.92 x 10(-2) U mg protein(-1) at an initial pH of 7.5 in organic medium. Apparent kinetic parameters (V'(max), K'(m), k'(cat) and k'(cat)/K'(m)) for the pseudoperoxidase activity were determined in organic media for different pH values from a Michaelis-Menten plot. Furthermore, the stability of the protein-calixarene complex was investigated for different initial pH values and half-life (t(1/2)) values were obtained in the range of 1.96 and 2.64 days. Hemoglobin-calixarene complex present in organic medium was recovered in fresh aqueous solutions at alkaline pH, with a recovery of pseudoperoxidase activity of over 100%. These results strongly suggest that the use of calixarene derivatives is an alternative technique for protein extraction and solubilisation in organic media for biocatalysis.

Divulgação sobre dispêndios de pesquisa e desenvolvimento em Portugal no ano de 2011: uma análise do PSI20

Mestrado em Contabilidade

As remisturas satíricas no YouTube: criatividade e subversão nas lutas de poder simbólico e cultural

O YouTube é descrito como uma “comunidade” em que os seus membros fazem vídeos em conjunto, vêem as criações vídeo uns dos outros, são inspirados por esses vídeos, comentam-nos e partilham-nos. Este artigo foca-se nas práticas vídeo de remistura desenvolvidas no campo da política, no seu sentido mais estrito, envolvendo actores e temáticas tradicionalmente consideradas desta esfera, mas também no sentido mais lato, enquanto respeitante às lutas de poder que marcam a vida social, especialmente no que se refere às lutas de poder simbólico e cultural. A remistura com fins de crítica política proporciona a exposição tanto das estratégias dos actores políticos, como do funcionamento interno dos media e das relações entre ambos. Estas práticas vídeo contributivas implicam dois processos distintos: primeiro, a partilha de um vasto quadro referencial, ligando a construção de sentido a um carácter intertextual no ambiente online; e segundo, procedente da digitalização, a transformação de imagens, palavras e sons em elementos prontos a utilizar de uma linguagem multimédia que conduz a formas avançadas de pastiche e paródia.

Media digitais e transformações na comunicação pública e científica

Este número especial da revista Estudos em Comunicação/Communication Studies é dedicado ao tema “Media digitais e transformações na comunicação pública e científica” e visa divulgar resultados do projecto “Mutação dos Media: Transformações da comunicação pública e científica” (PTDC/CCI-COM/100765/2008), financiado pela FCT com verbas do Orçamento de Estado e desenvolvido de Fevereiro de 2010 a Julho de 2013.

Joining the online video conversation? Discourse and practices of European political institutions and politicians on YouTube

To mimic the online practices of citizens has been declared an imperative to improve communication and extend participation. This paper seeks to contribute to the understanding of how European discourses praising online video as a communication tool have been translated into actual practices by politicians, governments and organisations. By contrasting official documents with YouTube activity, it is argued that new opportunities for European political communication are far from being fully embraced, much akin to the early years of websites. The main choice has been to use YouTube channels fundamentally for distribution and archiving, thus neglecting its social media features. The disabling of comments by many heads of state and prime ministers - and, in 2010, the European Commission - indicates such an attitude. The few attempts made to foster citizen engagement, in particular during elections, have had limited success, given low participation numbers and lack of argument exchange.

Calix[4]arene-carbazole-containing polymers: synthesis and properties

New highly fluorescent calix[4]arene-containing phenylene-alt-ethynylene-3,6- and 2,7-carbazolylene polymers (CALIX-PPE-CBZs) have been synthesized for the first time and their photophysical properties evaluated. Both polymers were obtained in good isolated yields (70-84%), having M-w ranging from 7660-26,700 g mol(-1). It was found that the diethynyl substitution (3,6- or 2,7-) pattern on the carbazole monomers markedly influences the degree of polymerization. The amorphous yellow polymers are freely soluble in several nonprotic organic solvents and have excellent film forming abilities. TG/DSC analysis evidences similar thermal behaviors for both polymers despite their quite different molecular weight distributions and main-chain connectivities (T-g, in the range 83-95 degrees C and decomposition onsets around 270 degrees C). The different conjugation lengths attained by the two polymers dictates much of their photophysical properties. Thus, whereas the fully conjugated CALIX-PPE-2,7-CBZ has its emission maximum at 430 nm (E-g = 2.84 eV; Phi(F) = 0.62, CHCl3), the 3,6-linked counterpart (CALIX-PPE-3,6-CBZ) fluoresces at 403 nm with a significant lower quantum yield (E-g = 3.06 eV; Phi(F) = 0.31, CHCl3). The optical properties of both polymers are predominantly governed by the intrachain electronic properties of the conjugated backbones owing to the presence of calix[4]arenes along the polymer chain which disfavor significant interchain interactions, either in fluid- or solid-state.