An image processing application for quantification of protein aggregates in Caenorhabditis elegans

Protein aggregation became a widely accepted marker of many polyQ disorders, including Machado-Joseph disease (MJD), and is often used as readout for disease progression and development of therapeutic strategies. The lack of good platforms to rapidly quantify protein aggregates in a wide range of disease animal models prompted us to generate a novel image processing application that automatically identifies and quantifies the aggregates in a standardized and operator-independent manner. We propose here a novel image processing tool to quantify the protein aggregates in a Caenorhabditis elegans (C. elegans) model of MJD. Confocal mi-croscopy images were obtained from animals of different genetic conditions. The image processing application was developed using MeVisLab as a platform to pro-cess, analyse and visualize the images obtained from those animals. All segmenta-tion algorithms were based on intensity pixel levels.The quantification of area or numbers of aggregates per total body area, as well as the number of aggregates per animal were shown to be reliable and reproducible measures of protein aggrega-tion in C. elegans. The results obtained were consistent with the levels of aggrega-tion observed in the images. In conclusion, this novel imaging processing applica-tion allows the non-biased, reliable and high throughput quantification of protein aggregates in a C. elegans model of MJD, which may contribute to a significant improvement on the prognosis of treatment effectiveness for this group of disor-ders

Application to quantify fetal lung branching on rat explants

Recently, regulating mechanisms of branching morphogenesis of fetal lung rat explants have been an essential tool for molecular research. The development of accurate and reliable segmentation techniques may be essential to improve research outcomes. This work presents an image processing method to measure the perimeter and area of lung branches on fetal rat explants. The algorithm starts by reducing the noise corrupting the image with a pre-processing stage. The outcome is input to a watershed operation that automatically segments the image into primitive regions. Then, an image pixel is selected within the lung explant epithelial, allowing a region growing between neighbouring watershed regions. This growing process is controlled by a statistical distribution of each region. When compared with manual segmentation, the results show the same tendency for lung development. High similarities were harder to obtain in the last two days of culture, due to the increased number of peripheral airway buds and complexity of lung architecture. However, using semiautomatic measurements, the standard deviation was lower and the results between independent researchers were more coherent

Neural Electrode Array Based on Aluminum: Fabrication and Characterization

A unique neural electrode design is proposed with 3 mm long shafts made from an aluminum-based substrate. The electrode is composed by 100 individualized shafts in a 10 × 10 matrix, in which each aluminum shafts are precisely machined via dicing-saw cutting programs. The result is a bulk structure of aluminum with 65 ° angle sharp tips. Each electrode tip is covered by an iridium oxide thin film layer (ionic transducer) via pulsed sputtering, that provides a stable and a reversible behavior for recording/stimulation purposes, a 40 mC/cm2 charge capacity and a 145 Ω impedance in a wide frequency range of interest (10 Hz-100 kHz). Because of the non-biocompatibility issue that characterizes aluminum, an anodization process is performed that forms an aluminum oxide layer around the aluminum substrate. The result is a passivation layer fully biocompatible that furthermore, enhances the mechanical properties by increasing the robustness of the electrode. For a successful electrode insertion, a 1.1 N load is required. The resultant electrode is a feasible alternative to silicon-based electrode solutions, avoiding the complexity of its fabrication methods and limitations, and increasing the electrode performance.

Do silêncio à acção.

From silence to action: silence taken as a foundational action that allows creative processes. It is a blankness that precedes the moment of creation but also stands for a search for perfection, the un-representable, the Sublime. The ‘art moment’ is that what is created from such emptiness, in the vertiginous abyss from nowhere to action. We will work this complex process of understanding if an action precedes the silence or if the silence precedes the action. Analyzing art process, considering its phases, from the tension between either to create or not to create will be related with the potency refereed by Agamben regarding Bartleby: starting with blankness, with the invisibility of potency, when communication is urgent. When “the blues” are called into participation, when the melancholic state installs the potency of the work both to grow or be doubted, of to do or not to do, how do we read agency? Is silence the action? Should the action rest in silence? What to do? Remain in the state of pure potency or prove the impossibility to create form? Nowadays, the pensive image counters the logic of the narrative action. On the one hand, the image extends the action on the other hand suspends any given presumption. The artistic process carries on, in its poetic freedom, a search without the enslavement of representational forms.

Mapping Culture and Compromised Art in the Era of Globalization

This work presents an analysis of the cultural and artistic field, positively compromised with social and political questions. The authors start with the categorization of the idea of culture and move to vindication art movements. These movements, which followed the first vanguards and worked from the compromise with “otherness”, are at the origin of the contemporary denomination of political art. In this context, the authors approach the origins of activist art, referring to issues of gender, multiculturalism, globalization, and poverty. The different forms of presenting content are also an object of analysis: from art tradition to the contamination of daily life, from local to global, from street contact to digital.

Lições de Direito e Processo Penal, 2012/2013

§ Parte I A: DIREITO PENAL: CAPÍTULO I – CONCEITO DE DIREITO PENAL, COM ESPECIAL RELEVO DA DIFERENCIAÇÃO ENTRE DIREITO PENAL CLÁSSICO, DIREITO PENAL ECONÓMICO E SOCIAL E DIREITO DAS CONTRA-ORDENAÇÕES; CAPÍTULO II – O PROBLEMA DOS FINS DAS PENAS; CAPÍTULO III – BREVE ABORDAGEM DA EVOLUÇÃO HISTÓRICA DO DIREITO PENAL; CAPÍTULO IV – TEORIA GERAL DA LEI CRIMINAL; CAPÍTULO V – TEORIA GERAL DA INFRACÇÃO CRIMINAL: A) ELEMENTOS; B) CONSTRUÇÕES; C) ANÁLISE: 1) ACÇÃO; 2) TIPICIDADE; 3) ILICITUDE; 4) CULPA; 5) PUNIBILIDADE; CAPÍTULO VI – FORMAS DO CRIME:A) TENTATIVA; B) AUTORIA E COMPARTICIPAÇÃO; C) CONCURSO DE CRIMES § Parte I BDIREITO PROCESSUAL PENAL; CAPÍTULO I – A DELIMITAÇÃO DO DIREITO PROCESSUAL PENAL; CAPÍTULO II – OS PRINCÍPIOS FUNDAMENTAIS DO PROCESSO PENAL; CAPÍTULO III - A LEI PROCESSUAL PENAL E A SUA APLICAÇÃO; Parte II: CAPÍTULO I- OS SUJEITOS DO PROCESSO; CAPÍTULO II - O OBJECTO DO PROCESSO; CAPÍTULO III - AS MEDIDAS DE COACÇÃO E DE GARANTIA PATRIMONIAL; CAPÍTULO IV - TRAMITAÇÃO PROCESSUAL PENAL. § § Part I: CRIMINAL LAW: CHAPTER I - CONCEPT OF CRIMINAL LAW, WITH SPECIAL RELIEF OF THE DIFFERENTIATION BETWEEN CLASSIC CRIMINAL LAW, ECONOMIC AND SOCIAL CRIMINAL LAW AND THE “AGAINST ORDINANCES” LAW ("LAW OFFENSES"); CHAPTER II - THE PROBLEM OF THE ENDS OF THE PENALTIES; CHAPTER III - BRIEF OVERVIEW OF THE EVOLUTION HISTORY OF CRIMINAL LAW; CHAPTER IV - GENERAL THEORY OF THE CRIMINAL LAW; CHAPTER V - GENERAL THEORY OF THE CRIMINAL INFRACTION: A) ELEMENTS; B) CONSTRUCTIONS; C) ANALYSIS: 1) ACTION; 2) VAGUENESS DOCTRINE; 3) ILLEGALITY; 4) GUILT (FAULT); 5) PUNISHMENT; CHAPTER VI - FORMS OF THE CRIME: A) ATTEMPT; B) AUTHORSHIP AND "CO-PARTICIPATION"; C) CUMULATION OF OFFENCES. § Part I - B: CRIMINAL PROCEDURAL LAW: CHAPTER I - THE DELIMITATION OF THE CRIMINAL PROCEDURAL LAW; CHAPTER II - THE BASIC PRINCIPLES OF THE CRIMINAL PROCEDURE; CHAPTER III - THE CRIMINAL PROCEDURE LAW AND ITS APPLICATION; Part II: CHAPTER I - THE FIGURES OF THE PROCESS; CHAPTER II - THE OBJECT OF THE PROCESS; CHAPTER III - THE MEASURES OF COERCION AND PATRIMONIAL GUARANTEE (WARRANTY); CHAPTER IV - CRIMINAL PROCEDURE.

Lições de Direito e Processo Penal, 2013/2014

§ Parte I A: DIREITO PENAL: CAPÍTULO I – CONCEITO DE DIREITO PENAL, COM ESPECIAL RELEVO DA DIFERENCIAÇÃO ENTRE DIREITO PENAL CLÁSSICO, DIREITO PENAL ECONÓMICO E SOCIAL E DIREITO DAS CONTRA-ORDENAÇÕES; CAPÍTULO II – O PROBLEMA DOS FINS DAS PENAS; CAPÍTULO III – BREVE ABORDAGEM DA EVOLUÇÃO HISTÓRICA DO DIREITO PENAL; CAPÍTULO IV – TEORIA GERAL DA LEI CRIMINAL; CAPÍTULO V – TEORIA GERAL DA INFRACÇÃO CRIMINAL: A) ELEMENTOS; B) CONSTRUÇÕES; C) ANÁLISE: 1) ACÇÃO; 2) TIPICIDADE; 3) ILICITUDE; 4) CULPA; 5) PUNIBILIDADE; CAPÍTULO VI – FORMAS DO CRIME: A) TENTATIVA; B) AUTORIA E COMPARTICIPAÇÃO; C) CONCURSO DE CRIMES § Parte I B: DIREITO PROCESSUAL PENAL; CAPÍTULO I – A DELIMITAÇÃO DO DIREITO PROCESSUAL PENAL; CAPÍTULO II – OS PRINCÍPIOS FUNDAMENTAIS DO PROCESSO PENAL; CAPÍTULO III - A LEI PROCESSUAL PENAL E A SUA APLICAÇÃO; Parte II: CAPÍTULO I - OS SUJEITOS DO PROCESSO; CAPÍTULO II - O OBJECTO DO PROCESSO; CAPÍTULO III - AS MEDIDAS DE COACÇÃO E DE GARANTIA PATRIMONIAL; CAPÍTULO IV - TRAMITAÇÃO PROCESSUAL PENAL. § § Part I: CRIMINAL LAW: CHAPTER I - CONCEPT OF CRIMINAL LAW, WITH SPECIAL RELIEF OF THE DIFFERENTIATION BETWEEN CLASSIC CRIMINAL LAW, ECONOMIC AND SOCIAL CRIMINAL LAW AND THE “AGAINST ORDINANCES” LAW ("LAW OFFENSES"); CHAPTER II - THE PROBLEM OF THE ENDS OF THE PENALTIES; CHAPTER III - BRIEF OVERVIEW OF THE EVOLUTION HISTORY OF CRIMINAL LAW; CHAPTER IV - GENERAL THEORY OF THE CRIMINAL LAW; CHAPTER V - GENERAL THEORY OF THE CRIMINAL INFRACTION: A) ELEMENTS; B) CONSTRUCTIONS; C) ANALYSIS: 1) ACTION; 2) VAGUENESS DOCTRINE; 3) ILLEGALITY; 4) GUILT (FAULT); 5) PUNISHMENT; CHAPTER VI - FORMS OF THE CRIME: A) ATTEMPT; B) AUTHORSHIP AND "CO-PARTICIPATION"; C) CUMULATION OF OFFENCES. § Part I - B: CRIMINAL PROCEDURAL LAW: CHAPTER I - THE DELIMITATION OF THE CRIMINAL PROCEDURAL LAW; CHAPTER II - THE BASIC PRINCIPLES OF THE CRIMINAL PROCEDURE; CHAPTER III - THE CRIMINAL PROCEDURE LAW AND ITS APPLICATION; Part II: CHAPTER I - THE FIGURES OF THE PROCESS; CHAPTER II - THE OBJECT OF THE PROCESS; CHAPTER III - THE MEASURES OF COERCION AND PATRIMONIAL GUARANTEE (WARRANTY); CHAPTER IV -CRIMINAL PROCEDURE.

Application to quantify fetal lung branching on rat explant

Recently, regulating mechanisms of branching morphogenesis of fetal lung rat explants have been an essential tool for molecular research. The development of accurate and reliable segmentation techniques may be essential to improve research outcomes. This work presents an image processing method to measure the perimeter and area of lung branches on fetal rat explants. The algorithm starts by reducing the noise corrupting the image with a pre-processing stage. The outcome is input to a watershed operation that automatically segments the image into primitive regions. Then, an image pixel is selected within the lung explant epithelial, allowing a region growing between neighbouring watershed regions. This growing process is controlled by a statistical distribution of each region. When compared with manual segmentation, the results show the same tendency for lung development. High similarities were harder to obtain in the last two days of culture, due to the increased number of peripheral airway buds and complexity of lung architecture. However, using semiautomatic measurements, the standard deviation was lower and the results between independent researchers were more coherent.

Application of Gage R&R methodology for improving the training and evaluation of automatic optical inspection machine operators

The current level of demand by customers in the electronics industry requires the production of parts with an extremely high level of reliability and quality to ensure complete confidence on the end customer. Automatic Optical Inspection (AOI) machines have an important role in the monitoring and detection of errors during the manufacturing process for printed circuit boards. These machines present images of products with probable assembly mistakes to an operator and him decide whether the product has a real defect or if in turn this was an automated false detection. Operator training is an important aspect for obtaining a lower rate of evaluation failure by the operator and consequently a lower rate of actual defects that slip through to the following processes. The Gage R&R methodology for attributes is part of a Six Sigma strategy to examine the repeatability and reproducibility of an evaluation system, thus giving important feedback on the suitability of each operator in classifying defects. This methodology was already applied in several industry sectors and services at different processes, with excellent results in the evaluation of subjective parameters. An application for training operators of AOI machines was developed, in order to be able to check their fitness and improve future evaluation performance. This application will provide a better understanding of the specific training needs for each operator, and also to accompany the evolution of the training program for new components which in turn present additional new difficulties for the operator evaluation. The use of this application will contribute to reduce the number of defects misclassified by the operators that are passed on to the following steps in the productive process. This defect reduction will also contribute to the continuous improvement of the operator evaluation performance, which is seen as a quality management goal.

Anotação sintética, numa perspectiva de Direito Público-penal e Direito Privado-civil, ao Acórdão do Supremo Tribunal de Justiça Português, de 19 de Abril de 2012-- A poluição sonora e o direito humano ao descanso e à saúde, sem descurar a necessidade duma adequada política tributária.

Resumo: 1 – Sumário do Acórdão do Supremo Tribunal de Justiça, de 19 de Abril de 2012; 2 – Texto completo do Acórdão do Supremo Tribunal de Justiça, de 19 de Abril de 2012: cfr. http://www.dgsi.pt/jstj.nsf/954f0ce6ad9dd8b980256b5f003fa814/fc664c231f3e73cf802579ea003d91d2?OpenDocument&Highlight=0,polui%C3%A7%C3%A3o , 2 de Junho de 2012; 3 – Anotação sintética; 3.1 – Introdução à anotação sintética e suas características neste caso concreto; 4 – Algumas referências constitucionais centrais em relação a Direitos humanos e, nomeadamente, a um Direito humano a um meio-ambiente sadio, saudável em todas as suas vertentes e sentidos – o exemplo central do artigo 9.º da CRP; 4.1 – Algumas referências constitucionais centrais em relação a Direitos humanos e, nomeadamente, a um Direito humano a um meio-ambiente sadio, saudável em todas as suas vertentes e sentidos – o exemplo central do artigo 66.º da CRP e o Regime Geral do Ruído; 5 – O direito humano ao descanso e à saúde, rectius o direito ao ambiente sadio vs o direito ao lazer e/ou exploração económica de indústrias de diversão, rectius o direito à liberdade de iniciativa económica privada; 6 – A violação do direito humano, de personalidade, ao descanso e à saúde, rectius o direito a um ambiente sadio, numa perspectiva de Direito privado e Direito civil; 7 – A criminalização da poluição, designadamente a criminalização da poluição sonora – uma perspectiva de Direito público e Direito penal; 8 - A necessidade duma adequada política tributária que compatibilize desenvolvimento sustentado com a protecção dum meio ambiente sadio e com qualidade de vida; 9 – Conclusões. § Abstract: 1 - Summary of the Judgment of the Supreme Court of April 19, 2012, 2 - Complete text of the Judgment of the Supreme Court of April 19, 2012: cf. http://www.dgsi.pt/jstj.nsf/954f0ce6ad9dd8b980256b5f003fa814/fc664c231f3e73cf802579ea003d91d2?OpenDocument&Highlight=0,polui%C3%A7%C3%A3o , June 2, 2012, 3 - Synthetic Note: 3.1 - Introduction to synthetic annotation and its characteristics in this case 4 - Some references constitutional power over human rights and in particular to a human right to a healthy environment, healthy in all its forms and meanings - the central example of Article 9. of CRP; 4.1 - Some references constitutional power over human rights and in particular to a human right to a healthy environment, healthy in all its forms and meanings - the central example of Article 66. No of CRP and the General Noise; 5 - the human right to rest and health, rectius the right to healthy environment vs. the right to leisure and / or economic exploitation of industries fun, rectius the right to freedom of private economic initiative; 6 - the violation of human personality, to rest and health, rectius the right to a healthy environment, a perspective of private law and civil law; 7 - criminalization of pollution, including the criminalization of noise - a perspective of public law and criminal law; 8 - the need for appropriate tax policy that reconciles sustainable development with the protection of a healthy environment and quality of life; 9 - Conclusions.

Tribunal Constitucional Internacional – auto de ciência

Resumo: a criação dum Tribunal Constitucional Internacional, no nosso entender, é uma exigência da própria Declaração Universal dos Direitos do Homem de 1948. Neste texto são apresentados uma série de argumentos velhos e novos para a importância da existência do Tribunal Constitucional Internacional a nível mundial. Trata-se dum Tribunal que é indispensável à defesa dos Direitos do Homem, contra qualquer tipo de ditadura ou fanatismo económico, social, político, cultural ou mental. § Abstract: the creation of an International Constitutional Court, in our view, is a requirement of own Universal Declaration of Human Rights of 1948. In this paper are given a lot of old and new arguments for the importance of the existence of the International Constitutional Court worldwide. It is of a Court that is indispensable to the defense of human rights, against all forms of dictatorship or economic fanaticism, social, political, cultural or mental.