Osteoblast, fibroblast and in vivo biological response to poly(vinylidene fluoride) based composite materials

Electroactivematerials can be taken to advantage for the development of sensors and actuators as well as for novel tissue engineering strategies. Composites based on poly(vinylidenefluoride),PVDF,have been evaluated with respect to their biological response. Cell viability and proliferation were performed in vitro both with Mesenchymal Stem Cells differentiated to osteoblasts and Human Fibroblast Foreskin 1. In vivo tests were also performed using 6-week-old C57Bl/6 mice. It was concluded that zeolite and clay composites are biocompatible materials promoting cell response and not showing in vivo pro-inflammatory effects which renders both of them attractive for biological applications and tissue engineering, opening interesting perspectives to development of scaffolds from these composites. Ferrite and silver nanoparticle composites decrease osteoblast cell viability and carbon nanotubes decrease fibroblast viability. Further, carbon nanotube composites result in a significant increase in local vascularization accompanied an increase of inflammatory markers after implantation.

Design de estratégias de registos documentais participativos: Ações a implementar no Centro Histórico do Porto

Nesta comunicação apresentamos as metodologias utilizadas no trabalho de documentação participativa que está a ser desenvolvido no programa Manobras no Porto, no âmbito do projeto de investigação “The Museum of All: Práticas de Comunicação Institucional num Mundo de Redes Participativas”. Numa altura em que nos deparamos com grandes transformações económicas, sociais e culturais nas nossas sociedades, acreditamos que o trabalho de construção de um arquivo documental sobre os aspectos que caracterizam uma determinada cultura local, visando proteger o seu património imaterial, tornar-se-á tão profícuo, quanto maior for o envolvimento dos próprios membros dessa cultura, no processo. No caso de estudo que aqui apresentamos, pretendemos compreender de que modo é que a documentação audiovisual participativa poderá vir a contribuir para o reforço da capacidade coletiva e envolvimento dos cidadãos locais no processo de revitalização social, económica e cultural do Centro Histórico do Porto. Depois de uma primeira fase de trabalho de campo de auscultação, levada a cabo através de um processo de observação participante, iremos implementar novas estratégias de recolha e criação coletiva com o objetivo de proporcionar um processo de documentação mais envolvente e participativo.

Design de Ações Culturais Participativas: um Caso de Estudo no Centro Histórico da Cidade do Porto

In this paper we present the methodologies and preliminary conclusions of the first phase of the work of construction of audiovisual narratives related to the project “Manobras no Porto”, which will serve as a case study for the main research project entitled: “The Museum of All: Institutional Communication Practices in a Participatory Networked World”. The main objective of this research project is to understand how the use of collective and participatory creation of identities and narratives can contribute to get audiences engaged with cultural institutions and events. We intend to identify the effects and measure the dynamics of participation of audiences in the construction of audiovisual objects, and understand how these may influence the reconfiguration of the missions of the institutions and cultural projects, in the development of societies.

Collecting system percutaneous access using real-time tracking sensors: first pig model in vivo experience

Purpose: Precise needle puncture of the renal collecting system is an essential but challenging step for successful percutaneous nephrolithotomy. We evaluated the efficiency of a new real-time electromagnetic tracking system for in vivo kidney puncture. Materials and Methods: Six anesthetized female pigs underwent ureterorenoscopy to place a catheter with an electromagnetic tracking sensor into the desired puncture site and ascertain puncture success. A tracked needle with a similar electromagnetic tracking sensor was subsequently navigated into the sensor in the catheter. Four punctures were performed by each of 2 surgeons in each pig, including 1 each in the kidney, middle ureter, and right and left sides. Outcome measurements were the number of attempts and the time needed to evaluate the virtual trajectory and perform percutaneous puncture. Results: A total of 24 punctures were easily performed without complication. Surgeons required more time to evaluate the trajectory during ureteral than kidney puncture (median 15 seconds, range 14 to 18 vs 13, range 11 to 16, p ¼ 0.1). Median renal and ureteral puncture time was 19 (range 14 to 45) and 51 seconds (range 45 to 67), respectively (p ¼ 0.003). Two attempts were needed to achieve a successful ureteral puncture. The technique requires the presence of a renal stone for testing. Conclusions: The proposed electromagnetic tracking solution for renal collecting system puncture proved to be highly accurate, simple and quick. This method might represent a paradigm shift in percutaneous kidney access techniques

O contributo da arte participativa, para as práticas de organização do espaço público: "Um caso de estudo na freguesia de Cedofeita no Porto"

O presente projecto pretende analisar o factor participativo da arte praticada em espaço público na cidade do Porto, enquanto estímulo de transformação dos contextos socioculturais. A partir da criação do projecto prático Imagin’OPorto e da respectiva implementação de um conjunto de iniciativas contextuais na Freguesia de Cedofeita da cidade do Porto, pretende-se reactivar o papel activo do cidadão no espaço público. Esta forma de investigação, executada através de um formato prático, permitiu a criação de obras artísticas permanentes nos espaços públicos desta zona da cidade, de modo a focar o estudo num conjunto de novos conceitos e mudanças de paradigmas que estão associados a este tipo de expressão artística. Esta prática teve o objectivo de contribuir para a dissolução de barreiras entre diferentes grupos urbanos, promovendo a sua coexistência, bem como também potenciar o debate entre os cidadãos e instituições, sobre a utilização do espaço público.

Collecting system percutaneous access using real-time tracking sensors: first pig model in vivo experience

Purpose: Precise needle puncture of the renal collecting system is an essential but challenging step for successful percutaneous nephrolithotomy. We evaluated the efficiency of a new real-time electromagnetic tracking system for in vivo kidney puncture. Materials and Methods: Six anesthetized female pigs underwent ureterorenoscopy to place a catheter with an electromagnetic tracking sensor into the desired puncture site and ascertain puncture success. A tracked needle with a similar electromagnetic tracking sensor was subsequently navigated into the sensor in the catheter. Four punctures were performed by each of 2 surgeons in each pig, including 1 each in the kidney, middle ureter, and right and left sides. Outcome measurements were the number of attempts and the time needed to evaluate the virtual trajectory and perform percutaneous puncture. Results: A total of 24 punctures were easily performed without complication. Surgeons required more time to evaluate the trajectory during ureteral than kidney puncture (median 15 seconds, range 14 to 18 vs 13, range 11 to 16, p ¼ 0.1). Median renal and ureteral puncture time was 19 (range 14 to 45) and 51 seconds (range 45 to 67), respectively (p ¼ 0.003). Two attempts were needed to achieve a successful ureteral puncture. The technique requires the presence of a renal stone for testing. Conclusions: The proposed electromagnetic tracking solution for renal collecting system puncture proved to be highly accurate, simple and quick. This method might represent a paradigm shift in percutaneous kidney access techniques.

Targeting lactate transport suppresses in vivo breast tumour growth

Background: Most cancers, including breast cancer, have high rates of glucose consumption, associated with lactate production, a process referred as “Warburg effect”. Acidification of the tumour microenvironment by lactate extrusion, performed by lactate transporters (MCTs), is associated with higher cell proliferation, migration, invasion, angiogenesis and increased cell survival. Previously, we have described MCT1 up-regulation in breast carcinoma samples and demonstrated the importance of in vitro MCT inhibition. In this study, we performed siRNA knockdown of MCT1 and MCT4 in basal-like breast cancer cells in both normoxia and hypoxia conditions to validate the potential of lactate transport inhibition in breast cancer treatment. Results: The effect of MCT knockdown was evaluated on lactate efflux, proliferation, cell biomass, migration and invasion and induction of tumour xenografts in nude mice. MCT knockdown led to a decrease in in vitro tumour cell aggressiveness, with decreased lactate transport, cell proliferation, migration and invasion and, importantly, to an inhibition of in vivo tumour formation and growth. Conclusions: This work supports MCTs as promising targets in cancer therapy, demonstrates the contribution of MCTs to cancer cell aggressiveness and, more importantly, shows, for the first time, the disruption of in vivo breast tumour growth by targeting lactate transport.