1 resultado para Strathcona and Mount Royal, Donald Alexander Smith, Baron, 1820-1914.
em Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP)
Filtro por publicador
- Aberdeen University (5)
- Abertay Research Collections - Abertay University’s repository (2)
- Aberystwyth University Repository - Reino Unido (1)
- Adam Mickiewicz University Repository (2)
- Applied Math and Science Education Repository - Washington - USA (1)
- Aquatic Commons (7)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (1)
- Aston University Research Archive (10)
- Biblioteca Digital | Sistema Integrado de Documentación | UNCuyo - UNCUYO. UNIVERSIDAD NACIONAL DE CUYO. (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (1)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (1)
- Biblioteca Valenciana Digital - Ministerio de Educación, Cultura y Deporte - Valencia - Espanha (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (8)
- Boston College Law School, Boston College (BC), United States (1)
- Boston University Digital Common (4)
- Brock University, Canada (18)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- Cambridge University Engineering Department Publications Database (7)
- CentAUR: Central Archive University of Reading - UK (22)
- Center for Jewish History Digital Collections (8)
- Chapman University Digital Commons - CA - USA (6)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (11)
- Coffee Science - Universidade Federal de Lavras (1)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (1)
- CORA - Cork Open Research Archive - University College Cork - Ireland (3)
- Deakin Research Online - Australia (24)
- Digital Archives@Colby (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (4)
- DigitalCommons - The University of Maine Research (2)
- DigitalCommons@The Texas Medical Center (2)
- Duke University (1)
- Ecology and Society (1)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (5)
- Gallica, Bibliotheque Numerique - Bibliothèque nationale de France (French National Library) (BnF), France (1)
- Glasgow Theses Service (1)
- Greenwich Academic Literature Archive - UK (4)
- Harvard University (21)
- Helda - Digital Repository of University of Helsinki (12)
- Helvia: Repositorio Institucional de la Universidad de Córdoba (1)
- Indian Institute of Science - Bangalore - Índia (5)
- Memoria Académica - FaHCE, UNLP - Argentina (9)
- Memorial University Research Repository (1)
- Ministerio de Cultura, Spain (2)
- National Center for Biotechnology Information - NCBI (1)
- Open University Netherlands (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (4)
- Portal de Revistas Científicas Complutenses - Espanha (5)
- Publishing Network for Geoscientific & Environmental Data (84)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (55)
- Queensland University of Technology - ePrints Archive (65)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositorio Institucional de la Universidad de El Salvador (37)
- Repositorio Institucional de la Universidad Nacional Agraria (13)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (10)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (1)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- School of Medicine, Washington University, United States (1)
- Universidad de Alicante (2)
- Universidad del Rosario, Colombia (10)
- Universidad Politécnica de Madrid (4)
- Universidade de Lisboa - Repositório Aberto (3)
- Universidade Federal do Pará (1)
- Universidade Metodista de São Paulo (1)
- Universitat de Girona, Spain (1)
- Université de Lausanne, Switzerland (1)
- Université de Montréal (2)
- Université de Montréal, Canada (11)
- University of Innsbruck Digital Library - Austria (1)
- University of Michigan (385)
- University of Queensland eSpace - Australia (9)
- University of Southampton, United Kingdom (1)
- University of Washington (3)
- USA Library of Congress (1)
- WestminsterResearch - UK (3)
- Worcester Research and Publications - Worcester Research and Publications - UK (2)
Resumo:
Cells are able to detect and respond to mechanical cues from their environment. Previous studies have investigated this mechanosensitivity on various cell types, including neural cells such as astrocytes. In this study, we have carefully optimized polyacrylamide gels, commonly used as compliant growth substrates, considering their homogeneity in surface topography, mechanical properties, and coating density, and identified several potential pitfalls for the purpose of mechanosensitivity studies. The resulting astrocyte response to growth on substrates with shear storage moduli of G` = 100 Pa and G` = 10 kPa was then evaluated as a function of coating density of poly-D-lysine using quantitative morphometric analysis. Astrocytes cultured on stiff substrates showed significantly increased perimeter, area, diameter, elongation, number of extremities and overall complexity if compared to those cultured on compliant substrates. A statistically significant difference in the overall morphological score was confirmed with an artificial intelligence-based shape analysis. The dependence of the cells` morphology on PDL coating density seemed to be weak compared to the effect of the substrate stiffness and was slightly biphasic, with a maximum at 10-100 mu g ml(-1) PDL concentration. Our finding suggests that the compliance of the surrounding tissue in vivo may influence astrocyte morphology and behavior.