1 resultado para Reciprocal patchiness of resources
em Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP)
Filtro por publicador
- KUPS-Datenbank - Universität zu Köln - Kölner UniversitätsPublikationsServer (1)
- Repository Napier (1)
- Aberystwyth University Repository - Reino Unido (3)
- Academic Research Repository at Institute of Developing Economies (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (10)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Aquatic Commons (39)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (1)
- Archive of European Integration (24)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (11)
- Aston University Research Archive (37)
- Avian Conservation and Ecology - Eletronic Cientific Hournal - Écologie et conservation des oiseaux: (3)
- Biblioteca de Teses e Dissertações da USP (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (8)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (1)
- Biblioteca Digital de la Universidad Católica Argentina (4)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (41)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (27)
- Boston University Digital Common (2)
- Brock University, Canada (3)
- Bucknell University Digital Commons - Pensilvania - USA (3)
- Bulgarian Digital Mathematics Library at IMI-BAS (3)
- CaltechTHESIS (2)
- Cambridge University Engineering Department Publications Database (9)
- CentAUR: Central Archive University of Reading - UK (34)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (13)
- CiencIPCA - Instituto Politécnico do Cávado e do Ave, Portugal (1)
- Cochin University of Science & Technology (CUSAT), India (7)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (23)
- CORA - Cork Open Research Archive - University College Cork - Ireland (3)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (5)
- Dalarna University College Electronic Archive (4)
- Deakin Research Online - Australia (78)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (3)
- Digital Archives@Colby (1)
- Digital Commons - Michigan Tech (3)
- Digital Commons @ DU | University of Denver Research (2)
- Digital Commons at Florida International University (9)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (9)
- DigitalCommons@University of Nebraska - Lincoln (1)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (1)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (3)
- Ecology and Society (1)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (9)
- Escola Superior de Educação de Paula Frassinetti (1)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Glasgow Theses Service (2)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (27)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (44)
- Institutional Repository of Leibniz University Hannover (1)
- Instituto Politécnico de Bragança (1)
- Instituto Politécnico do Porto, Portugal (2)
- Iowa Publications Online (IPO) - State Library, State of Iowa (Iowa), United States (1)
- Memoria Académica - FaHCE, UNLP - Argentina (3)
- Memorial University Research Repository (1)
- National Center for Biotechnology Information - NCBI (12)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (3)
- Portal de Revistas Científicas Complutenses - Espanha (2)
- Publishing Network for Geoscientific & Environmental Data (6)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (38)
- Queensland University of Technology - ePrints Archive (164)
- Repositorio Académico de la Universidad Nacional de Costa Rica (3)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (3)
- Repositório Científico da Universidade de Évora - Portugal (4)
- Repositório digital da Fundação Getúlio Vargas - FGV (9)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (1)
- Repositório Institucional da Universidade de Aveiro - Portugal (2)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (24)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (4)
- School of Medicine, Washington University, United States (2)
- Scielo España (1)
- Universidad de Alicante (5)
- Universidad del Rosario, Colombia (4)
- Universidad Politécnica de Madrid (16)
- Universidade Complutense de Madrid (3)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade Federal do Pará (2)
- Universitat de Girona, Spain (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (4)
- Université de Lausanne, Switzerland (1)
- Université de Montréal, Canada (7)
- Université Laval Mémoires et thèses électroniques (1)
- University of Canberra Research Repository - Australia (1)
- University of Michigan (23)
- University of Queensland eSpace - Australia (10)
- University of Southampton, United Kingdom (4)
- University of Washington (4)
- WestminsterResearch - UK (1)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
Tuberculosis (TB) is one of the most common infectious diseases known to man and responsible for millions of human deaths in the world. The increasing incidence of TB in developing countries, the proliferation of multidrug resistant strains, and the absence of resources for treatment have highlighted the need of developing new drugs against TB. The shikimate pathway leads to the biosynthesis of chorismate, a precursor of aromatic amino acids. This pathway is absent from mammals and shown to be essential for the survival of Mycobacterium tuberculosis, the causative agent of TB. Accordingly, enzymes of aromatic amino acid biosynthesis pathway represent promising targets for structure-based drug design. The first reaction in phenylalanine biosynthesis involves the conversion of chorismate to prephenate, catalyzed by chorismate mutase. The second reaction is catalyzed by prephenate dehydratase (PDT) and involves decarboxylation and dehydratation of prephenate to form phenylpyruvate, the precursor of phenylalanine. Here, we describe utilization of different techniques to infer the structure of M. tuberculosis PDT (MtbPDT) in solution. Small angle X-ray scattering and ultracentrifugation analysis showed that the protein oligomeric state is a tetramer and MtbPDT is a flat disk protein. Bioinformatics tools were used to infer the structure of MtbPDT A molecular model for MtbPDT is presented and molecular dynamics simulations indicate that MtbPDT i.s stable. Experimental and molecular modeling results were in agreement and provide evidence for a tetrameric state of MtbPDT in solution.