Modulation of a cytoskeletal calpain-like protein induces major transitions in trypanosome morphology

Individual eukaryotic microbes, such as the kinetoplastid parasite Trypanosoma brucei, have a defined size, shape, and form yet transition through life cycle stages, each having a distinct morphology. In questioning the structural processes involved in these transitions, we have identified a large calpain-like protein that contains numerous GM6 repeats (ClpGM6) involved in determining T. brucei cell shape, size, and form. ClpGM6 is a cytoskeletal protein located within the flagellum along the flagellar attachment zone (FAZ). Depletion of ClpGM6 in trypomastigote forms produces cells with long free flagella and a shorter FAZ, accompanied by repositioning of the basal body, the kinetoplast, Golgi, and flagellar pocket, reflecting an epimastigote-like morphology. Hence, major changes in microbial cell form can be achieved by simple modulation of one or a few proteins via coordinated association and positioning of membrane and cytoskeletal components.

Dark Places Catalogue (First ed.)

Exhibition Catalogue of Dark Places. As one of four co-curators, I worked with John Hansard to produce the publication. The catalogue contains additional information on artists, the positioning of the work with a commissioned essay by Sally O'Reilly. In addition to a descriptive entry on the Dark Places database,as Director of Office of Experiments we also designed Research Tools for independent researchers wishing to undertake work in the field. This included an ID card, with observational notes that correspond to the taxonomy of the database and a researched guide to legal issues for documenting and photographing secret sites.