1 resultado para Limits of Sets
em Worcester Research and Publications - Worcester Research and Publications - UK
Filtro por publicador
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- Aberystwyth University Repository - Reino Unido (5)
- Academic Archive On-line (Jönköping University; Sweden) (1)
- Aquatic Commons (19)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (1)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (2)
- Archive of European Integration (6)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (4)
- Aston University Research Archive (10)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (14)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (34)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (24)
- Brock University, Canada (7)
- Brunel University (1)
- Bucknell University Digital Commons - Pensilvania - USA (3)
- Bulgarian Digital Mathematics Library at IMI-BAS (2)
- CaltechTHESIS (5)
- Cambridge University Engineering Department Publications Database (14)
- CentAUR: Central Archive University of Reading - UK (48)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (45)
- Cochin University of Science & Technology (CUSAT), India (4)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (6)
- CORA - Cork Open Research Archive - University College Cork - Ireland (4)
- CUNY Academic Works (1)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (84)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (3)
- Digital Archives@Colby (3)
- Digital Commons @ DU | University of Denver Research (2)
- Digital Commons at Florida International University (4)
- DigitalCommons@University of Nebraska - Lincoln (1)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (8)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (4)
- Greenwich Academic Literature Archive - UK (4)
- Helda - Digital Repository of University of Helsinki (18)
- Indian Institute of Science - Bangalore - Índia (44)
- Institutional Repository of Leibniz University Hannover (1)
- Instituto Politécnico do Porto, Portugal (17)
- Massachusetts Institute of Technology (1)
- National Center for Biotechnology Information - NCBI (3)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (4)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (6)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (126)
- Queensland University of Technology - ePrints Archive (89)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositório Científico da Universidade de Évora - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (3)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (5)
- Repositorio Institucional da UFLA (RIUFLA) (6)
- Repositório Institucional da Universidade de Aveiro - Portugal (2)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (185)
- Research Open Access Repository of the University of East London. (1)
- SAPIENTIA - Universidade do Algarve - Portugal (1)
- Scientific Open-access Literature Archive and Repository (1)
- Universidad de Alicante (3)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (6)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade Federal do Pará (4)
- Universidade Federal do Rio Grande do Norte (UFRN) (1)
- Universitat de Girona, Spain (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (2)
- Université de Lausanne, Switzerland (7)
- Université de Montréal, Canada (24)
- University of Michigan (14)
- University of Queensland eSpace - Australia (9)
- University of Washington (4)
- WestminsterResearch - UK (6)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
A monoclonal antibody that recognises components of the wall of sporangia of Peronospora destructor was raised. Tests using spores of higher fungi and other species of mildew demonstrated the specificity of the monoclonal. The antibody was used to develop lateral flow devices for sporangia of P. destructor. A competitive lateral flow format was developed which could detect onion downy mildew sporangia. Five-microliter gold anti-mouse IgM solution pre-mixed with 10 μl of P. destructor monoclonal antibody (EMA 242) proved the optimal concentration for detection of sporangia of P. destructor when applied to sample pads of lateral flow devices. Limits of approximately 500 sporangia of P. destructor could be detected by the absence of a test line on the lateral flow device within test samples. Using a scanning densitometer improved the sensitivity of detection. Further development and validation of the test is required if it is to be used for risk assessments of onion downy mildew in the field.