The influence of promoters (Zr, La, Tb, Pr) on the catalytic performance of CuO-CeO2 systems for the preferential oxidation of CO in the presence of CO2 and H2O

CuO supported on CeO2 and Ce0.9X0.1O2, where X is Zr, La, Tb or Pr, were synthesized using nitrate precursors, giving rise ceria based materials with a small particle size which interact with CuO species generating a high amount of interfacial sites. The incorporation of cations to the ceria framework modifies the CeO2 lattice parameter, improving the redox behavior of the catalytic system. The catalysts were characterized by X-ray fluorescence spectrometry (XRFS), X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), Raman spectroscopy, thermoprogrammed reduction with H2 (H2-TPR) and X-ray photoelectron spectroscopy (XPS). The catalysts were tested in the preferential oxidation of CO under a H2-rich stream (CO-PROX), reaching conversion values higher than 95% between 115 and 140 °C and being the catalyst with 6 wt.% of Cu supported on Ce0.9Zr0.1O2 (sample 6CUZRCE) the most active catalyst. The influence of the presence of CO2 and H2O was also studied simulating a PROX unit, taking place a decrease of the catalytic activity due to the inhibitor effect both CO2 and H2O.

El auge de la promoción inmobiliaria en la franja litoral alicantina y sus efectos en la reestructuración de la oferta

Ante el fuerte crecimiento de la oferta turística en los municipios de la franja litoral alicantina, que se materializa en la construcción de más de 70.000 viviendas durante el quinquenio 1985-89, realiza un análisis de las variables que identifican esta promoción inmobiliaria (tipo de ordenación, origen de los promotores, tipo de inversión, valores ecológicos, calidad de vida, situación, tipo de vivienda, etc...) al tiempo que explica las repercusiones del fenómeno sobre la región. Finalmente, se señala el receso que desde 1989 viene padeciendo este sector y describe la situación pormenorizada de cada uno de los municipios estudiados.

Gender-sensitive reporting in medical research

Sex and gender differences influence the health and wellbeing of men and women. Although studies have drawn attention to observed differences between women and men across diseases, remarkably little research has been pursued to systematically investigate these underlying sex differences. Women continue to be underrepresented in clinical trials, and even in studies in which both men and women participate, systematic analysis of data to identify potential sex-based differences is lacking. Standards for reporting of clinical trials have been established to ensure provision of complete, transparent and critical information. An important step in addressing the gender imbalance would be inclusion of a gender perspective in the next Consolidated Standards of Reporting Trials (CONSORT) guideline revision. Uniform Requirements for Manuscripts Submitted to Biomedical Journals, as a set of well-recognized and widely used guidelines for authors and biomedical journals, should similarly emphasize the ethical obligation of authors to present data analyzed by gender as a matter of routine. Journal editors are also promoters of ethical research and adequate standards of reporting, and requirements for inclusion of gender analyses should be integrated into editorial policies as a matter of urgency.

Nitrogen regulation of protein–protein interactions and transcript levels of GlnK PII regulator and AmtB ammonium transporter homologs in Archaea

Gene homologs of GlnK PII regulators and AmtB-type ammonium transporters are often paired on prokaryotic genomes, suggesting these proteins share an ancient functional relationship. Here, we demonstrate for the first time in Archaea that GlnK associates with AmtB in membrane fractions after ammonium shock, thus, providing a further insight into GlnK-AmtB as an ancient nitrogen sensor pair. For this work, Haloferax mediterranei was advanced for study through the generation of a pyrE2-based counterselection system that was used for targeted gene deletion and expression of Flag-tagged proteins from their native promoters. AmtB1-Flag was detected in membrane fractions of cells grown on nitrate and was found to coimmunoprecipitate with GlnK after ammonium shock. Thus, in analogy to bacteria, the archaeal GlnK PII may block the AmtB1 ammonium transporter under nitrogen-rich conditions. In addition to this regulated protein–protein interaction, the archaeal amtB-glnK gene pairs were found to be highly regulated by nitrogen availability with transcript levels high under conditions of nitrogen limitation and low during nitrogen excess. While transcript levels of glnK-amtB are similarly regulated by nitrogen availability in bacteria, transcriptional regulators of the bacterial glnK promoter including activation by the two-component signal transduction proteins NtrC (GlnG, NRI) and NtrB (GlnL, NRII) and sigma factor σN (σ54) are not conserved in archaea suggesting a novel mechanism of transcriptional control.

Electrooxidation methods to produce pseudocapacitance-containing porous carbons

Surface oxygen groups play a key role on the performance of porous carbon electrodes for electrochemical capacitors in aqueous media. The electrooxidation method in NaCl electrolyte using a filter press cell and dimensionally stable anodes is proposed as a viable process for the generation of oxygen groups on porous carbon materials. The experimental set-up is so flexible that allows the easy modification of carbon materials with different configurations, i.e. cloths and granular, obtaining different degrees of oxidation for both conformations without the requirement of binders and conductivity promoters. After the electrooxidation method, the attained porosity is maintained between 90 and 75% of the initial values. The surface oxygen groups generated can increase the capacitance up to a 30% when compared to the pristine material. However, a severe oxidation is detrimental since it may decrease the conductivity and increase the resistance for ion mobility.

Solvent-Free Enantioselective Organocatalyzed Aldol Reactions

The use of proline as catalyst for the aldol process has given a boost to the development of organocatalysis as a research area. Since then, a plethora of organocatalysts of diverse structures have been developed for this and other organic transformations under different reaction conditions. The use of an organic molecule as catalyst to promote a reaction meets several principles of Green Chemistry. The implementation of solvent-free methodologies to carry out the aldol reaction was soon envisaged. These solvent-free processes can be performed using conventional magnetic stirring or applying ball milling techniques and are even compatible with the use of supported organocatalysts as promoters, which allows the recovery and reuse of the organocatalysts. In addition, other advantages such as the reduction of the required amount of nucleophile and the acceleration of the reaction are accomplished by using solvent-free conditions leading to a “greener” and more sustainable process.

La Universidad de Alicante Universidad promotora de salud/Universidad Saludable. Un reto y una oportunidad

Justificación: Dentro de la estrategia de Universidades Saludables, la Universidad de Alicante (UA) inicia un proyecto para conocer, difundir y potenciar los activos para la salud. Se plantea dotar de contenido empírico la propuesta de Morgan y Ziglio de usar el modelo de activos para la salud pública identificados por la comunidad universitaria. Objetivos: explorar la factibilidad y los retos de la aplicación de mapeos de activos para la salud en la UA con el fin de que la comunidad universitaria pueda ganar salud, calidad de vida y bienestar. Desarrollo de la experiencia: Formación de promotores de salud: • La promoción de la salud y la teoría salutogénica. • Aproximación al modelo y la estrategia de activos en salud. Enfoque Asset-Based Community Development (ABCD). • Diferencia entre recursos y activos. • Técnicas de observación y diálogo. • Técnicas mixtas: open space, TICs y mapping... • Competencias profesionales, entorno facilitador y apoyo para obtener resultados. Metodología: Lograr el mapeo de los activos en salud, sus entornos y sus estudiantes siguiendo el enfoque de John McKnight. Aplicación de “lo aprendido” en el contexto de la UA: Planificación del proyecto para el año 2014. Resultados: construcción de un mapa de activos para la salud, geolocalizado en la Universidad de Alicante. Dinamización del mapa de activos, estudiando conexiones entre activos y necesidades de la comunidad universitaria con las personas participantes, para realizar propuestas de acción futura. Difusión del mismo a través de tecnologías de la información.

Análisis de la información en la prevención del consumo de drogas y otras adicciones

Tradicionalmente gran parte de los programas preventivos de drogas y otras adicciones dirigidos a preadolescentes y adolescentes, se han apoyado en la información como estrategia fundamental para disuadir el consumo, utilizando argumentos basados en las consecuencias del deterioro al ser consumidores de sustancias, así como en el abuso de otras conductas adictivas no químicas. El enfoque clásico se apoya en que los jóvenes toman sus decisiones de consumir, o no, en base a una elaboración racional, por tanto en el caso de estar informados de los riesgos que asumen al consumir optarían por no hacerlo. Es fundamental incidir en la importancia que adquieren tres cuestiones alrededor de la información: el nivel y calidad de la información que tienen los jóvenes, el nivel y calidad de la información que tienen los padres y la búsqueda de las fuentes de información sobre drogas y su nivel de credibilidad. Posiblemente los modelos de transmisión y contenido de información que utiliza la publicidad comercial podrían ser más adecuados y eficaces que los del recurso al miedo en el ámbito de los comportamientos de salud, y por supuesto, de las adicciones en general. La tendencia de los promotores de mensajes de salud sigue siendo la de transmitir las consecuencias y los riesgos, en la línea de la apelación al miedo. Presumimos que se alcanzaría una mayor eficiencia con mensajes positivos, reforzando los estilos de vida saludables.

Enhanced electro-oxidation resistance of carbon electrodes induced by phosphorus surface groups

The electro-oxidation of carbon materials enormously degrades their performance and limits their wider utilization in multiple electrochemical applications. In this work, the positive influence of phosphorus functionalities on the overall electrochemical stability of carbon materials has been demonstrated under different conditions. We show that the extent and selectivity of electroxidation in P-containing carbons are completely different to those observed in conventional carbons without P. The electro-oxidation of P-containing carbons involves the active participation of phosphorus surface groups, which are gradually transformed at high potentials from less-to more-oxidized species to slow down the introduction of oxygen groups on the carbon surface (oxidation) and the subsequent generation of (C*OOH)-like unstable promoters of electro-gasification. The highest-oxidized P groups (–C–O–P-like species) seem to distribute the gained oxygen to neighboring carbon sites, which finally suffer oxidation and/or gasification. So it is thought that P-groups could act as mediators of carbon oxidation although including various steps and intermediates compared to electroxidation in P-free materials.

Dual regulation of P-glycoprotein expression by Trichostatin A in cancer cell lines

Background. It has been reported that the histone deacetylase inhibitor (iHDAc) trichostatin A (TSA) induces an increase in MDR1 gene transcription (ABCB1). This result would compromise the use of iHDACs in combination with other cytotoxic agents that are substrates of P-glycoprotein (Pgp). It has also been reported the use of alternative promoters by the ABCB1 gene and the existence of a traslational control of Pgp protein. Finally, the ABCB1 gene is located in a genetic locus with the nested gene RUNDC3B in the complementary DNA strand, raising the possibility that RUNDC3B expression could interfere with ABCB1 alternative promoter regulation. Methods. A combination of RT-PCR, real time RT-PCR, Western blot and drug accumulation assays by flow cytometry have been used in this study. Results. The iHDACs-induced increase in MDR1 mRNA levels is not followed by a subsequent increase in Pgp protein levels or activity in several pancreatic and colon carcinoma cell lines, suggesting a traslational control of Pgp in these cell lines. In addition, the MDR1 mRNA produced in these cell lines is shorter in its 5' end that the Pgp mRNA produced in cell lines expressing Pgp protein. The different size of the Pgp mRNA is due to the use of alternative promoters. We also demonstrate that these promoters are differentially regulated by TSA. The translational blockade of Pgp mRNA in the pancreatic carcinoma cell lines could be related to alterations in the 5' end of the MDR1 mRNA in the Pgp protein expressing cell lines. In addition, we demonstrate that the ABCB1 nested gene RUNDC3B expression although upregulated by TSA is independent of the ABCB1 alternative promoter used. Conclusions. The results show that the increase in MDR1 mRNA expression after iHDACs treatment is clinically irrelevant since this mRNA does not render an active Pgp protein, at least in colon and pancreatic cancer cell lines. Furthermore, we have demonstrated that TSA in fact, differentially regulates both ABCB1 promoters, downregulating the upstream promoter that is responsible for active P-glycoprotein expression. These results suggest that iHDACs such as TSA may in fact potentiate the effects of antitumoral drugs that are substrates of Pgp. Finally, we have also demonstrate that TSA upregulates RUNDC3B mRNA independently of the ABCB1 promoter in use.

Las ONG como agentes de transformación social. Del asistencialismo a la movilización

Este estudio de caso se centra en la campaña #dereitos365, desarrollada entre diciembre de 2014 y enero de 2015 en Galicia para reclamar una implicación ciudadana durante todo el año y no solo en la época navideña. Partiendo del potencial de las redes sociales e Internet y tomando este caso como referencia, el objetivo de la investigación es profundizar en el papel de las ONG como agentes de transformación social y como promotoras de un diálogo interactivo y multidireccional que implique a los nuevos ciudadanos. Los resultados muestran una campaña marcadamente institucional que busca la movilización y concienciación ciudadana para la construcción de una sociedad más justa y solidaria, generando visibilidad y cierta interactividad a través de mensajes reivindicativos, sobre todo por parte de organizaciones locales, y de un alto contenido multimedia.