A PCR based method to detect Russula spp. in soil samples and Limodorum abortivum roots in Mediterranean environments

Aim of study. Orchidaceae has the largest number of species of any family in the plant kingdom. This family is subject to a high risk of extinction in natural environments, such as natural parks and protected areas. Recent studies have shown the prevalence of many species of orchids to be linked to fungal soil diversity, due to their myco-heterotrophic behaviour. Plant communities determine fungal soil diversity, and both generate optimal conditions for orchid development. Area of study. The work was carried out in n the two most important natural parks in Alicante (Font Roja and Sierra Mariola), in South-eastern of Spain. Material and Methods. We designed a molecular tool to monitor the presence of Russula spp. in soil and orchids roots, combined with phytosociological methods. Main results. Using a PCR-based method, we detected the presence in the soil and Limodorum abortivum orchid roots of the mycorrhizal fungi Russula spp. The species with highest coverage was Quercus rotundifolia in areas where the orchid was present. Research highlights. We present a useful tool based on PCR to detect the presence of Russula spp. in a natural environment. These results are consistent with those obtained in different studies that linked the presence of the mycorrhizal fungi Russula spp. in roots of the species Limodorum and the interaction between these fungal species and Quercus ilex trees in Mediterranean forest environments.

La aplicación de las medidas correctivas de control de la estabilidad presupuestaria y sostenibilidad financiera a las subvenciones

A través del presente trabajo se pretende analizar algunas de las medidas correctivas que sobre la Administración pública impone la LOEPSF. En concreto, la contenida en el art. 20.3 de la citada Ley, que obliga a recabar un informe del Ministerio de Hacienda para la percepción de subvenciones públicas a los entes del sector público integrados en Comunidades Autónomas que no hayan cumplido los objetivos de déficit. El trabajo defiende una interpretación restrictiva del citado precepto sobre la base del análisis del presupuesto de hecho de la norma y su naturaleza de norma sancionadora.