The identity of sociology or what to do when the universe is unknown: qualitative solutions against the quantitative obsession

Social Sciences can, on occasions, be similar to the so called “hard” sciences. However, in many cases, neither the object nor the classical methods fit in with the objectives of the work. The object requires methodological and technical adjustments, which are often avoided by means of an improper rigidity of the object’s needs. These adjustments can even alter the original research idea. The main objective of this article consists of proving that those objects of study, less suitable to be addressed by rigid positivistic strategies, can be approached both scientifically and sociologically. This can be achieved with the use of different strategies and flexible methodologies to ensure validity and reliability standards. This paper will be posed, firstly, a reflection on the epistemological nature of the debate about the rigid-flexible perspectives. Secondly, the strategies and tools used by the research team to achieve the reduction of the uncertainty about the size and characteristics of the population studied will be described. Finally, some of the survey results obtained in this project will be compared to those provided by the FAMILITUR Survey (2008), conducted by the Spanish Institute of Tourist Studies (IET).

Chiral β-amino alcohols as ligands for the ruthenium-catalyzed asymmetric transfer hydrogenation of N-phosphinyl ketimines

Some chiral β-amino alcohols have been evaluated as potential ligands for the ruthenium-catalyzed asymmetric transfer hydrogenation (ATH) of N-phosphinyl ketimines in isopropyl alcohol. The ruthenium complex prepared from [RuCl2(p-cymene)]2 and (1S,2R)-1-amino-2-indanol has shown to be an efficient catalyst for the ATH of several N-(diphenylphosphinyl)imines, affording the reduction products in very good isolated yields and enantiomeric excesses up to 82%. The inherent rigidity of the indane ring system present in the ligand seems to be very important to achieve good enantioselectivities.

Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

Glutaraldehyde is one of the most widely used reagents in the design of biocatalysts. It is a powerful crosslinker, able to react with itself, with the advantages that this may bring forth. In this review, we intend to give a general vision of its potential and the precautions that must be taken when using this effective reagent. First, the chemistry of the glutaraldehyde/amino reaction will be commented upon. This reaction is still not fully clarified, but it seems to be based on the formation of 6-membered heterocycles formed by 5 C and one O. Then, we will discuss the production of intra- and inter-molecular enzyme crosslinks (increasing enzyme rigidity or preventing subunit dissociation in multimeric enzymes). Special emphasis will be placed on the preparation of cross-linked enzyme aggregates (CLEAs), mainly in enzymes that have low density of surface reactive groups and, therefore, may be problematic to obtain a final solid catalyst. Next, we will comment on the uses of glutaraldehyde in enzymes previously immobilized on supports. First, the treatment of enzymes immobilized on supports that cannot react with glutaraldehyde (only inter and intramolecular cross-linkings will be possible) to prevent enzyme leakage and obtain some enzyme stabilization via cross-linking. Second, the cross-linking of enzymes adsorbed on aminated supports, where together with other reactions enzyme/support crosslinking is also possible; the enzyme is incorporated into the support. Finally, we will present the use of aminated supports preactivated with glutaraldehyde. Optimal glutaraldehyde modifications will be discussed in each specific case (one or two glutaraldehyde molecules for amino group in the support and/or the protein). Using preactivated supports, the heterofunctional nature of the supports will be highlighted, with the drawbacks and advantages that the heterofunctionality may have. Particular attention will be paid to the control of the first event that causes the immobilization depending on the experimental conditions to alter the enzyme orientation regarding the support surface. Thus, glutaraldehyde, an apparently old fashioned reactive, remains the most widely used and with broadest application possibilities among the compounds used for the design of biocatalyst.

Hydrogen Peroxide in Biocatalysis. A Dangerous Liaison

Hydrogen peroxide is a substrate or side-product in many enzyme-catalyzed reactions. For example, it is a side-product of oxidases, resulting from the re-oxidation of FAD with molecular oxygen, and it is a substrate for peroxidases and other enzymes. However, hydrogen peroxide is able to chemically modify the peptide core of the enzymes it interacts with, and also to produce the oxidation of some cofactors and prostetic groups (e.g., the hemo group). Thus, the development of strategies that may permit to increase the stability of enzymes in the presence of this deleterious reagent is an interesting target. This enhancement in enzyme stability has been attempted following almost all available strategies: site-directed mutagenesis (eliminating the most reactive moieties), medium engineering (using stabilizers), immobilization and chemical modification (trying to generate hydrophobic environments surrounding the enzyme, to confer higher rigidity to the protein or to generate oxidation-resistant groups), or the use of systems capable of decomposing hydrogen peroxide under very mild conditions. If hydrogen peroxide is just a side-product, its immediate removal has been reported to be the best solution. In some cases, when hydrogen peroxide is the substrate and its decomposition is not a sensible solution, researchers coupled one enzyme generating hydrogen peroxide “in situ” to the target enzyme resulting in a continuous supply of this reagent at low concentrations thus preventing enzyme inactivation. This review will focus on the general role of hydrogen peroxide in biocatalysis, the main mechanisms of enzyme inactivation produced by this reactive and the different strategies used to prevent enzyme inactivation caused by this “dangerous liaison”.

Permanencia y mutaciones del Estado napoleónico bajo la Restauración de los Borbones, 1814-1830

El regreso de los Borbones en 1814, a pesar del agresivo programa de extrema derecha que trajo consigo, según el cual pretendía acabar con el aparato del Estado centralizado por considerarlo la fuente misma del “peligro revolucionario”, no mermó en ningún caso el poder de una administración que había sido muy poco depurada. El realismo ultra rápidamente demostró su incapacidad para estabilizar la economía y la sociedad mediante un compromiso histórico que, sin embargo, fue rápidamente organizado por una tecnocracia compuesta por una fusión de los despachos de la Francia anterior a 1789 y los del episodio revolucionario e imperial. Entre lecciones de Turgot y de Corbert, el Estado se hizo el guardián y el tutor de un liberalismo económico y social que dotó de un nuevo impuso a la industrialización de Francia y a la formación de un mercado nacional protegido. Esta identidad modernizadora del Estado supo evolucionar. Sobrevivió al giro a la derecha de la vida política propiciado por Villèle y, para hacer frente a las dificultades que se encontró Francia en su modernización a partir de 1828, el Estado se propuso aprender de los “métodos ingleses” que hicieron evolucionar la rigidez de la administración napoleónica.

Cálculo del asiento elástico tridimensional de cimentaciones de rigidez variable en terrenos con una capa rígida inclinada

Las fórmulas basadas en la teoría de la elasticidad son ampliamente utilizadas para el cálculo de asientos de cimentaciones, ya que la totalidad de la normativa geotécnica recomienda su empleo. No obstante, estos métodos no cubren todas las situaciones geotécnicamente posibles ya que frecuentemente las condiciones geológicas son complejas. En este trabajo se analiza la influencia de la presencia de una capa rígida inclinada en los asientos elásticos de una cimentación superficial. Para ello se han resuelto 273 modelos tridimensionales no lineales de elementos finitos, variando los parámetros clave del problema: la inclinación y la profundidad de la capa rígida y la rigidez de la cimentación. Finalmente, se ha realizado un análisis estadístico de los resultados de los modelos y se ha propuesto una fórmula que puede ser utilizada en el cálculo de asientos por métodos elásticos, para tener en consideración la presencia de una capa rígida inclinada en profundidad.