1 resultado para Gonadal maturation
em Universidad de Alicante
Filtro por publicador
- University of Cagliari UniCA Eprints (1)
- Aberdeen University (2)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (8)
- Adam Mickiewicz University Repository (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (4)
- Aquatic Commons (110)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (1)
- Archimer: Archive de l'Institut francais de recherche pour l'exploitation de la mer (3)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (5)
- Aston University Research Archive (6)
- B-Digital - Universidade Fernando Pessoa - Portugal (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (19)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (5)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (30)
- Bioline International (2)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (37)
- Boston University Digital Common (1)
- Brock University, Canada (3)
- Bucknell University Digital Commons - Pensilvania - USA (1)
- CaltechTHESIS (5)
- Cambridge University Engineering Department Publications Database (6)
- CentAUR: Central Archive University of Reading - UK (19)
- Centro Hospitalar do Porto (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (78)
- Cochin University of Science & Technology (CUSAT), India (6)
- CORA - Cork Open Research Archive - University College Cork - Ireland (2)
- Deakin Research Online - Australia (34)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (9)
- Digital Commons at Florida International University (1)
- DigitalCommons@The Texas Medical Center (13)
- DigitalCommons@University of Nebraska - Lincoln (1)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (14)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (18)
- FAUBA DIGITAL: Repositorio institucional científico y académico de la Facultad de Agronomia de la Universidad de Buenos Aires (1)
- FUNDAJ - Fundação Joaquim Nabuco (1)
- Greenwich Academic Literature Archive - UK (1)
- Helda - Digital Repository of University of Helsinki (48)
- Illinois Digital Environment for Access to Learning and Scholarship Repository (1)
- Indian Institute of Science - Bangalore - Índia (45)
- INSTITUTO DE PESQUISAS ENERGÉTICAS E NUCLEARES (IPEN) - Repositório Digital da Produção Técnico Científica - BibliotecaTerezine Arantes Ferra (1)
- Instituto Superior de Psicologia Aplicada - Lisboa (1)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- National Center for Biotechnology Information - NCBI (39)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (3)
- Portal de Revistas Científicas Complutenses - Espanha (1)
- Publishing Network for Geoscientific & Environmental Data (5)
- QSpace: Queen's University - Canada (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (39)
- Queensland University of Technology - ePrints Archive (72)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (6)
- Repositório Digital da UNIVERSIDADE DA MADEIRA - Portugal (2)
- REPOSITORIO DIGITAL IMARPE - INSTITUTO DEL MAR DEL PERÚ, Peru (19)
- Repositório Institucional da Universidade Federal do Rio Grande do Norte (3)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (149)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (2)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (2)
- School of Medicine, Washington University, United States (3)
- Universidad Autónoma de Nuevo León, Mexico (1)
- Universidad de Alicante (1)
- Universidad Politécnica de Madrid (4)
- Universidade de Lisboa - Repositório Aberto (1)
- Universidade de Madeira (1)
- Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) (4)
- Universidade Federal do Pará (9)
- Universidade Federal do Rio Grande do Norte (UFRN) (11)
- Universitat de Girona, Spain (1)
- Université de Lausanne, Switzerland (4)
- Université de Montréal (5)
- Université de Montréal, Canada (13)
- University of Michigan (2)
- University of Queensland eSpace - Australia (13)
Resumo:
The green Cu-NirK from Haloferax mediterranei (Cu-NirK) has been expressed, refolded and retrieved as a trimeric enzyme using an expression method developed for halophilic Archaea. This method utilizes Haloferax volcanii as a halophilic host and an expression vector with a constitutive and strong promoter. The enzymatic activity of recombinant Cu-NirK was detected in both cellular fractions (cytoplasmic fraction and membranes) and in the culture media. The characterization of the enzyme isolated from the cytoplasmic fraction as well as the culture media revealed important differences in the primary structure of both forms indicating that Hfx. mediterranei could carry out a maturation and exportation process within the cell before the protein is exported to the S-layer. Several conserved signals found in Cu-NirK from Hfx. mediterranei sequence indicate that these processes are closely related to the Tat system. Furthermore, the N-terminal sequence of the two Cu-NirK subunits constituting different isoforms revealed that translation of this protein could begin at two different points, identifying two possible start codons. The hypothesis proposed in this work for halophilic Cu-NirK processing and exportation via the Tat system represents the first approximation of this mechanism in the Halobacteriaceae family and in Prokarya in general.