Sequencing and functional analysis of the genome of a nematode egg-parasitic fungus, Pochonia chlamydosporia

Pochonia chlamydosporia is a worldwide-distributed soil fungus with a great capacity to infect and destroy the eggs and kill females of plant-parasitic nematodes. Additionally, it has the ability to colonize endophytically roots of economically-important crop plants, thereby promoting their growth and eliciting plant defenses. This multitrophic behavior makes P. chlamydosporia a potentially useful tool for sustainable agriculture approaches. We sequenced and assembled ∼41 Mb of P. chlamydosporia genomic DNA and predicted 12,122 gene models, of which many were homologous to genes of fungal pathogens of invertebrates and fungal plant pathogens. Predicted genes (65%) were functionally annotated according to Gene Ontology, and 16% of them found to share homology with genes in the Pathogen Host Interactions (PHI) database. The genome of this fungus is highly enriched in genes encoding hydrolytic enzymes, such as proteases, glycoside hydrolases and carbohydrate esterases. We used RNA-Seq technology in order to identify the genes expressed during endophytic behavior of P. chlamydosporia when colonizing barley roots. Functional annotation of these genes showed that hydrolytic enzymes and transporters are expressed during endophytism. This structural and functional analysis of the P. chlamydosporia genome provides a starting point for understanding the molecular mechanisms involved in the multitrophic lifestyle of this fungus. The genomic information provided here should also prove useful for enhancing the capabilities of this fungus as a biocontrol agent of plant-parasitic nematodes and as a plant growth-promoting organism.

Los procedimientos de revisión de oficio en la Ley General Tributaria y la recuperación de las ayudas de Estado

El trabajo analiza los posibles procedimientos de revisión en materia tributaria para proceder a la devolución de ayudas de Estado que se concedieron través de medidas tributarias favorables y que se declaran ilegales por parte de la UE.

A Belief System's Organization Based on a Computational Model of the Dynamic Context: First Approximation

In this article we present a model of organization of a belief system based on a set of binary recursive functions that characterize the dynamic context that modifies the beliefs. The initial beliefs are modeled by a set of two-bit words that grow, update, and generate other beliefs as the different experiences of the dynamic context appear. Reason is presented as an emergent effect of the experience on the beliefs. The system presents a layered structure that allows a functional organization of the belief system. Our approach seems suitable to model different ways of thinking and to apply to different realistic scenarios such as ideologies.

Walking Through Cantor's Paradise and Escher's Garden: Epistemological Reflections on the Mathematical Infinite (II)

Infinity is not an easy concept. A number of difficulties that people cope with when dealing with problems related to infinity include its abstract nature, understanding infinity as an ongoing, never ending process, understanding infinity as a set of an infinite number of elements and appreciating well-known paradoxes. Infinity can be understood in several ways with often incompatible meanings, and can involve value judgments or assumptions that are neither explicit nor desired. To usher in its definition, we distinguish several aspects, teleological, artistic (Escher); some definitive, some potential, and others actual. This article also deals with some still unresolved aspects of the concept of infinity.