Outdoor room, C House, Coorparoo, Brisbane

As seen from upper level of house.

Outdoor room, C House, Coorparoo, Brisbane

Looking towards pool deck, Hanging Basket top right.

Outdoor room, C House, Coorparoo, Brisbane

As seen from upper level of house.

Outdoor room, C House, Coorparoo, Brisbane

Looking towards Iwan from within outdoor room.

Outdoor room, Q House, Chelmer, Brisbane

View from front of property through main entrance to double-height outdoor room. Existing house on right.

Outdoor room, Kangaroo Point House, Kangaroo Point, Brisbane

Looking towards section of original house from outdoor room area. Hand-made spotted gum columns on edge of outdoor room on right.

Battened roof to outdoor room, Kangaroo Point House, Kangaroo Point, Brisbane

Timber battened concave roof and supporting structure over outdoor room area.

Outdoor room, Kangaroo Point House, Kangaroo Point, Brisbane

Dining setting, sculptures, timber columns and curved roof in outdoor room

Outdoor room, T House, Sunshine Coast

As seen from mezzanine.

Pavilions and outdoor seating area, Lake Weyba House, Sunshine Coast

As seen from back of bedroom pavilion, looking towards main pavilion. Day bed alcove to bedroom in foreground.

In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line

A continuous cell line, Aa23, was established from eggs of a strain of the Asian tiger mosquito, Aedes albopictus, naturally infected with the intracellular symbiont Wolbachia pipientis. The resulting cell line was shown to be persistently infected with the bacterial endosymbiont. Treatment with antibiotics cured the cells of the infection. In the course of establishing this cell line it was noticed that RFLPs in the PCR products of two Wolbachia genes from the parental mosquitoes were fixed in the infected cell line. This indicates that the mosquito host was naturally superinfected with different Wolbachia strains, whereas the infected cell line derived from these mosquitoes only contained one of the original Wolbachia strains. The development of anin vitroculture system for this fastidious microorganism should facilitate molecular analysis of the reproduction distorting phenotypes it induces in natural arthropod hosts.