Are US coral reefs on the slippery slope to slime?

Conservation of U.S. coral reefs has been sidetracked by the partial implementation of management plans without clearly achievable goals. Historical ecology reveals global patterns of coral reef degradation that provide a framework for reversing reef decline with ecologically meaningful metrics for success. The authors of this Policy Forum urge action now to address multiple threats simultaneously, because the harmful effects of stressors like overfishing, pollution, poor land-use practices, and global warming are interdependent. Prompt implementation of proven, practical solutions would lead to both short- and long-term benefits, including the return of keystone species and the economic benefits they entail.

Expression of growth factors in Dictyostelium discoideum

Growth factors and their binding proteins are important proteins regulating mammalian cell proliferation and differentiation so there is considerable interest in producing them as recombinant proteins, especially in hosts that do not already produce a complex mixture of growth factors. Many growth factors require posttranslational modifications making them unsuitable for production in Escherichia coli or other prokaryotes. Since several expression vector systems have been recently developed for foreign protein production in the cellular slime mould, Dictyostelium discoideum, we attempted to use two of these systems to express human insulin-like growth factor binding protein 6 (hIGFBP6) and bovine beta-cellulin (bBTC) as secreted proteins. Although both proteins were successfully produced in stably transformed amoebae, no secretion was detected in spite of several attempts to facilitate this occurring.