Entrance and verandah, Sunshine Coast University Library, Maroochydore

View to entrance, along timber batten screen to verandah.

North-east verandah connection, Sunshine Coast University Library, Maroochydore

View of underside of verandah corrugated steel roofing, polycarbonate sheeting and north-east glass and floor connection.

Vertical sunshades to south-west elevation, Sunshine Coast University Library, Maroochydore

View of vertical sunshade to south-west elevation with glazing behind.

North-east corner elevation, Sunshine Coast University Library, Maroochydore

View to north-east corner elevation, with entrance stair and timber batten screen to verandah.

Entrance stair, Sunshine Coast University Library, Maroochydore

View to entrance stair as seen from exterior court.

South elevation elevation, Sunshine Coast University Library, Maroochydore

View to south-east elevation with corrugated steel cladding, plywood, concrete block and colonnade, as seen from exterior.

South-east elevation, Sunshine Coast University Library, Maroochydore

View to south-east elevation; entrance stair, plywood and sheet steel cladding and colonnade, as seen from exterior.

Timber batten screen to north-east elevation, Sunshine Coast University Library, Maroochydore

View of timber batten screen to verandah behind and entrance stair, as seen from exterior.

Batten screen to north-east elevation, Sunshine Coast University Library, Maroochydore

View of timber batten screen to north-east elevation with verandah behind.

Screen to verandah, Sunshine Coast University Library, Maroochydore

View of steel-framed timber screen to verandah.

Second floor reading area interior, Sunshine Coast University Library, Maroochydore

View of second floor reading area with rigid frames and air-conditioning ducting.

South-east elevation, Sunshine Coast University Library, Maroochydore

View to south-east elevation as seen from exterior.

North-west elevation, Sunshine Coast University Library, Maroochydore

North-west elevation as seen from Building K.

Entrance elevation, Sunshine Coast University Library, Maroochydore

View to entrance verandah on north-east elevation and sunshades to north-west elevation.

Determination of Wolbachia genome size by Pulsed-Field Gel Electrophoresis

Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number of sites and were used for the determination of the genome sizes of wMelPop, wMel, and wMelCS (each 1.36 Mb), wRi (1.66 Mb), wBma (1.1 Mb), and wDim (0.95 Mb). The Wolbachia genomes studied were all much smaller than the genomes of free-living bacteria such as Escherichia coli (4.7 Mb), as is typical for obligate intracellular bacteria. There was considerable genome size variability among Wolbachia strains, especially between the more parasitic A group Wolbachia infections of insects and the mutualistic C and D group infections of nematodes. The studies described here found no evidence for extrachromosomal plasmid DNA in any of the strains examined. They also indicated that the Wolbachia genome is circular.