GFAP analysis in aluminium chloride-treated rat brain regions

The neurotoxicity of aluminium chloride was assessed in maleWistar albino rats. Rats were treated with aluminium chloride dissolved in distilled water at a dose of 300 mg/kg body weight daily by oral gavage (1 ml) for 45 days. Controls were treated with distilled water only. Animals were sacrificed and different brain regions were dissected. GFAP analysis was carried out by Western blotting using mouse anti-GFAP monoclonal antibody (Pharmingen: 60311D) at 1:1000. Blots were developed with HRP-linked goat anti-mouse secondary antibody and quantified by densitometry.