Co-localization of GnRH ligands and their receptors in the European sea bass, Dicentrarchus labrax

The migration of the hypophysiotropic GnRH (GnRH-I) neurons during early development is a crucial step in establishing a normally functioning reproductive system in all vertebrates. These neurons derive from progenitor cells in the olfactory placode and subsequently migrate to their final position in the ventral forebrain, where they mediate hypophysiotropic control over Lh. We use zebrafish as a model to investigate the path and the factors that mediate the migration of the GnRH-I neurons during early development. A transgenic line of zebrafish, in which GnRH- I neurons specifically express a reporter gene (GFP) has been developed in our lab. This was achieved by integrating a GnRH-I promoter/GFP reporter transgene into the zebrafish genome. The resulting transgenic line allows us to track the route of the GnRH-I neuronal migration in real time and in vivo. We have used this line to conduct time lapse imaging to ascertain the exact migrational path and the final position in the ventral forebrain of the GnRH-I neurons.

BitTorrent based transmission of real-time scalable video over P2P networks

The number of software applications available on the Internet for distributing video streams in real time over P2P networks has grown quickly in the last two years. Typical this kind of distribution is made by television channel broadcasters which try to make their content globally available, using viewer's resources to support a large scale distribution of video without incurring in incremental costs. However, the lack of adaptation in video quality, combined with the lack of a standard protocol for this kind of multimedia distribution has driven content providers to basically ignore it as a solution for video delivery over the Internet. While the scalable extension of the H. 264 encoding (H.264/SVC) can be used to support terminal and network heterogeneity, it is not clear how it can be integrated in a P2P overlay to form a large scale and real time distribution. In this paper, we start by defining a solution that combines the most popular P2P file-sharing protocol, the BitTorrent, with the H. 264/SVC encoding for a real-time video content delivery. Using this solution we then evaluate the effect of several parameters in the quality received by peers.