4 resultados para ZONE ELECTROPHORESIS

em SAPIENTIA - Universidade do Algarve - Portugal


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Dissertação de Mestrado, Gestão da Água e da Costa, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2009

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Dissertação de Mestrado, Gestão da Água e da Costa, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2010

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This special issue of Estuarine, Coastal and Shelf Science synthesizes and updates the developments in science related to Land Ocean Interactions in the Coastal Zone (LOICZ). Frequent updates about the dynamic coastal zone are useful and necessary as global change accelerates. There is an urgent need to improve the knowledge and understanding of the vulnerability of society and ecosystems to global change hazards in the coastal zone (Vermaat et al., 2005). The collection of papers in this special issue places new developments, findings, techniques and insights within the context of LOICZ science. For the convenience of the reader, the references to papers included in this special issue are printed in italic, whereas other references to LOICZ science are in normal print.

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The predominantly selfing slug species Arion (Carinarion) fasciatus, A. (C.) silvaticus and A. (C.) circumscriptus are native in Europe and have been introduced into North America, where each species consists of a single, homozygous multilocus genotype (strain), as defined by starch gel electrophoresis (SGE) of allozymes. In Europe, the “one strain per species” hypothesis does not hold since polyacrylamide gel electrophoresis (PAGE) of allozymes uncovered 46 strains divided over the three species. However, electrophoretic techniques may differ in their ability to detect allozyme variation. Therefore, several Carinarion populations from both continents were screened by applying the two techniques simultaneously on the same individual slugs and enzyme loci. SGE and PAGE yielded exactly the same results, so that the different degree of variation in North American and European populations cannot be attributed to differences in resolving power between SGE and PAGE. We found four A. (C.) silvaticus strains in North America indicating that in this region the “one strain per species” hypothesis also cannot be maintained. Hence, the discrepancies between previous electrophoretic studies on Carinarion are most likely due to sampling artefacts and possible founder effects.