24 resultados para Padrão esquelético
em SAPIENTIA - Universidade do Algarve - Portugal
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Relatório de projecto de licenciatura, Bioquímica, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2009
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In ecotoxicology a major focus is in the aquatic environment, not only because it presents a great economic value to man but it is an ecosystem widely affected by the growing anthropogenic pollution. Most of the studies performed relate to adverse effects in development, reproductive or endocrine disruption but little is known about the possible effects in bone formation and skeletal development. In this study, we set out to evaluate the effects of 8 aquatic pollutants on the skeletal development using an in vivo system, the zebrafish larvae aged 20 days post-fertilization, through chronic exposure. Several endpoints were considered such as the cumulative mortality, total length, occurrence of skeletal deformities and marker gene expression. We were able to establish LD50 values for some pollutants, like 3-methylcholanthrene, lindane, diclofenac, cobalt and vanadate and found that the total length was not affected by any of the pollutants tested. Cobalt was the most harmful chemical to affect hatching time, severely affecting the ability of the zebrafish embryos to hatch and overall the number of deformities increased upon exposure to tested chemicals but no patterns of deformities were identified. We also propose that 3-methylcholanthrene has an osteogenic effect, affecting osteoblast and osteoclast function and that op levels can act as a mediator of 3-methylcholanthrene toxic stress to the osteoblast. In turn we found naphthalene to probably have a chondrogenic effect. Our results provided new insights into the potential osteotoxicity of environmental pollutants. Future studies should aim at confirming these preliminary data and at determining mechanisms of osteotoxicity.
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Dissertação mest., Ciências Biomédicas, Universidade do Algarve, 2010
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Tese de dout., Ecologia, Faculdade de Ciências do Mar e do Ambiente, Universidade do Algarve, 2008
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Tese dout., Aquacultura, Universidade do Algarve, 2007
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Dissertação mest., Biologia Marinha, Universidade do Algarve, 2007
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Tese dout., Biologia, Universidade do Algarve, 2005
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Dissertação de mest., Biologia Marinha, Faculdade de Ciências do Mar e do Ambiente, Universidade do Algarve, 2007
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Dissertação de Mestrado, Biologia Marinha, Especialização em Ecologia e Conservação, Faculdade de Ciências do Mar e do Ambiente, Universidade do Algarve, 2007
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Dissertação de mest., Teoria e Método na Arqueologia, Faculdade de Ciências Humanas e Sociais, Universidade do Algarve, 2009
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Dissertação mest., Biologia marinha - Ecologia e conservação marinha, Universidade do Algarve, 2007
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Dissertação mest., Psicologia, Universidade do Algarve, 2008
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Dissertação de mest., Biologia Marinha, Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2009
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Dissertação mest., Biologia marinha, Universidade do Algarve, 2009
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Tese dout., Ciências e Tecnologias do Ambiente, 2009, Universidade do Algarve