Economical and operational optimization of CALB immobilization on MP1000 for amidation of olein fatty acids with ethanolamine

Dissertação de Mestrado , Engenharia Biológica, Faculdade de Engenharia de Recursos Naturais, Universidade do Algarve, 2008

Towards a hydrodynamic operational model of the Algarve coast and St. Vincent Cape

This study attempts to implement a hydrodynamic operational model which can ultimately be used for projecting oil spill dispersal patterns and also sewage, pollution and can also be used in wave forecasting. A two layer nested model was created using MOHID Water, which is powerful ocean modelling software. The first layer (father) is used to impose the boundary conditions for the second layer (son). This was repeated for two different wind dominant regimes, Easterly and Westerly winds respectively. A qualitative comparison was done between measured tidal data and the tidal output. Sea surface temperature was also qualitatively compared with the model’s results. The results from both simulations were analysed and compared to historical literature. The comparison was done at the surface layer, 100 metre depth and at 800m depth. In the surface layer the first simulation generated an upwelling event near Cape St. Vincent and within the Algarve. The second simulation generated a non-upwelling event within which the surface was flow reversed and the warm water mass was along the Algarve coastline and evening turning clockwise around Cape St. Vincent. At the 100 metre depth for both simulations, velocity vortexes were observed near Cape St. Vincent travelling northerly and southerly at various instances. At 800metre depth a strong oceanic flow was observed moving north westerly along the continental shelf.

Biochemical indices and life traits of loggerhead turtles (Caretta caretta) from Cape Verde Islands

The loggerhead turtle (Caretta caretta) is an endangered marine reptile for whom assessing population health requires knowledge of demographic parameters such as individual growth rate. In Cape Verde, as within several populations, adult female loggerhead sea turtles show a size-related behavioral and trophic dichotomy. While smaller females are associated with oceanic habitats, larger females tend to feed in neritic habitats, which is reflected in their physiological condition and in their offspring. The ratio of RNA/DNA provides a measure of cellular protein synthesis capacity, which varies depending on changes in environmental conditions such as temperature and food availability. The purpose of this study was to evaluate the combined use of morphometric data and biochemical indices as predictors of the physiological condition of the females of distinct sizes and hatchlings during their nesting season and how temperature may influence the physiological condition on the offspring. Here we employed biochemical indices based on nucleic acid derived indices (standardized RNA/DNA ratio-sRD, RNA concentration and DNA concentration) in skin tissue as a potential predictor of recent growth rate in nesting females and hatchling loggerhead turtles. Our major findings were that the physiological condition of all nesting females (sRD) decreased during the nesting season, but that females associated with neritic habitats had a higher physiological condition than females associated with oceanic habitats. In addition, the amount of time required for a hatchling to right itself was negatively correlated with its physiological condition (sRD) and shaded nests produced hatchlings with lower sRD. Overall, our results showed that nucleic acid concentrations and ratios of RNA to DNA are an important tool as potential biomarkers of recent growth in marine turtles. Hence, as biochemical indices of instantaneous growth are likely temperature-, size- and age-dependent, the utility and validation of these indices on marine turtles stocks deserves further study.

Development of an operational tool for oil spill forecast: application to oil exposed regions

Tese de doutoramento, Ciências do Mar, da Terra e do Ambiente (Modelação), Faculdade de Ciências e Tecnologia, Universidade do Algarve, 2014