Role of the different actin genes during extrusion of capping protein mutant cells in the Drosophila wing blade epithelium

Dissertação mest., Biotecnologia, Universidade do Algarve, 2008

Special section on intelligent control systems and signal processing

The IFAC International Conference on Intelligent Control Systems and Signal Processing (ICONS 2003) was organized under the auspices of the recently founded IFAC Technical Committee on Cognition and Control, and it was the first IFAC event specifically devoted to this theme. Recognizing the importance of soft-computing techniques for fields covered by other IFAC Technical Committees, ICONS 2003 was a multi-track Conference, co-sponsored by four additional Technical Committees: Computers for Control, Optimal Control, Control in Agriculture, and Modelling, Identification and Signal Processing. The Portuguese Society for Automatic Control (APCA) hosted ICONS 2003, which was held at the University of Algarve, Faro, Portugal.

Preface to the special section on algorithms and architectures for real-time control

Rapid developments in microelectronics and computer science continue to fuel new opportunities for real-time control engineers. The ever-increasing system complexity and sophistication, environmental legislation, economic competition, safety and reliability constitute some of the driving forces for the research themes presented at the IFAC Workshop on Algorithms and Architectures for Real-Time Control (AARTC'2000). The Spanish Society for Automatic Control hosted AARTC'2000, which was held at Palma de Maiorca, Spain, from 15 to 17 May. This workshop was the sixth in the series.

Evolution of matrix and bone gamma-carboxyglutamic acid proteins in vertebrates

The evolution of calcified tissues is a defining feature in vertebrate evolution. Investigating the evolution of proteins involved in tissue calcification should help elucidate how calcified tissues have evolved. The purpose of this study was to collect and compare sequences of matrix and bone γ-carboxyglutamic acid proteins (MGP and BGP, respectively) to identify common features and determine the evolutionary relationship between MGP and BGP. Thirteen cDNAs and genes were cloned using standard methods or reconstructed through the use of comparative genomics and data mining. These sequences were compared with available annotated sequences (a total of 48 complete or nearly complete sequences, 28 BGPs and 20 MGPs) have been identified across 32 different species (representing most classes of vertebrates), and evolutionarily conserved features in both MGP and BGP were analyzed using bioinformatic tools and the Tree-Puzzle software. We propose that: 1) MGP and BGP genes originated from two genome duplications that occurred around 500 and 400 million years ago before jawless and jawed fish evolved, respectively; 2) MGP appeared first concomitantly with the emergence of cartilaginous structures, and BGP appeared thereafter along with bony structures; and 3) BGP derives from MGP. We also propose a highly specific pattern definition for the Gla domain of BGP and MGP. Previous Section Next Section BGP1 (bone Gla protein or osteocalcin) and MGP (matrix Gla protein) belong to the growing family of vitamin K-dependent (VKD) proteins, the members of which are involved in a broad range of biological functions such as skeletogenesis and bone maintenance (BGP and MGP), hemostasis (prothrombin, clotting factors VII, IX, and X, and proteins C, S, and Z), growth control (gas6), and potentially signal transduction (proline-rich Gla proteins 1 and 2). VKD proteins are characterized by the presence of several Gla residues resulting from the post-translational vitamin K-dependent γ-carboxylation of specific glutamates, through which they can bind to calcium-containing mineral such as hydroxyapatite. To date, VKD proteins have only been clearly identified in vertebrates (1) although the presence of a γ-glutamyl carboxylase has been reported in the fruit fly Drosophila melanogaster (2) and in marine snails belonging to the genus Conus (3). Gla residues have also been found in neuropeptides from Conus venoms (4), suggesting a wider prevalence of γ-carboxylation.