Cloning and expression in Escherichia coli of full-length complementary DNA coding for human α1-antitrypsin

A cDNA library prepared from human liver was screened for α₁-antitrypsin, a major constituent of plasma which functions as inhibitor of proteolytic enzyms. The library was screened using a 12-base-long synthetic oligodeoxyribonucleotide corresponding to a known DNA fragment of human α₁-antitrypsin and by hybrid-selection of α₁-antitrypsin mRNA.

Evaluation of the effect of complementary pollination on Actinidia deliciosa 'Hayward' in northwest Portugal

The objective of this research was to study the effect of complementary pollination on kiwifruit production and quality. For 3 years, complementary application of wet or dry pollen have been done at different stages of flower opening on vines in the Portuguese regions of Entre-Douro e Minho and Beira Litoral. Commercial production data were collected and fruit quality attributes were measured at harvest. Complementary pollination did not affect fruit soluble solids content or firmness in any year, and was beneficial for fruit size and commercial production in the third year only, showing that it is important in some conditions, when natural pollination is inadequate.