Nanoparticle-based biosensors: development and application

Tese dout., Ciências Biotecnológicas, Universidade do Algarve, 2009

Role of insulin and insulin-like peptides in bone formation: identification of bone-specific target genes and regulatory mechanisms, and characterization of the insulin-mimetic effect of vanadium

Tese de Doutoramento em Biologia, Especialidade em Biologia Molecular, Universidade do Algarve, 2008

Bio-removal of toxic metals by metal resistant anaerobic bacteria: molecular characterization and performance studies

Tese de dout., Ciências Biotecnológicas (Biotecnologia Ambiental), Faculdade de Ciências e Tecnologia, Univ. do Algarve, 2010

Application of molecular tools for detection of plant viruses

Grapevine leafroll disease (GLRD) is one of the most important virus diseases of grapevines worldwide, causing major economical impact. The disease has a complex aetiology and currently eleven phloem-limited viruses, termed in general Grapevine leafroll-associated virus (GLRaVs), have been identified. Two of the GLRaVs, GLRaV-1 and GLRaV-3, are included in the European certification scheme of propagation material. However, the flawed notion that GLRaV-3 is more frequent than GLRaV-1 and that all other GLRaVs are possibly not as relevant for GLRD, has until now precluded the development of specific serological and molecular detection assays and limited the scope of molecular characterization of the viruses known to be associated with the disease. Hence, few studies have addressed the phylodynamics of GLRaVs or even characterized the genetic structure of their natural populations. This generalized lack of molecular information, in turn underlie the deficient capacity to detect the viruses. The phylogenetic analyses were conducted on the basis of the heat shock protein 70 homologue (HSP70h) and the coat protein (CP) genes for GLRaV-1 and the HSP70h, the heat shock protein 90 homologue (HSP90h) and the CP genes for GLRaV-5. The data obtained for GLRaV-1 contributed 83 new CP sequences. This information was combined with previous analysis by other authors and used for the production of new polyclonal IgG, capable of detecting CP variants from all the phylogroups observed. Successful testing of this new tool included tissue print immunoblotting (TPIB) and in situ immunoassay (ISIA). The data obtained for GLRaV-5, contributed 61 new CP and 28 new HSP90h gene sequences. Eight phylogenetic groups were identified on the basis of the CP. Characterization of the genetic structure of the isolates revealed a higher diversity than previously reported and allowed the identification of dominant virus variants. For both GLRaV-1 and GLRaV-5, the effect of vegetative propagation on the virus transmission dynamics was addressed.

Electrical characterization of organic based transistors: stability issues

An investigation into the stability of metal insulator semiconductor (MIS) transistors based on alpha-sexithiophene is reported. In particular the kinetics of the threshold voltage shift upon application of a gate bias has been determined. The kinetics follow a stretched-hyperbola type behavior, in agreement with the formalism developed to explain metastability in amorphous-silicon thin film transistors. Using this model, quantification of device stability is possible. Temperature-dependent measurements show that there are two processes involved in the threshold voltage shift, one occurring at T approximate to 220 K and the other at T approximate to 300 K. The latter process is found to be sample dependent. This suggests a relation between device stability and alpha-sexithiophene deposition parameters. Copyright (c) 2005 John Wiley A Sons, Ltd.

Framework for the characterization of hotel websites

Nowadays the importance of websites to the tourism and hospitality industries is fundamental. Hotels, for instance, spend a huge amount of money improving their websites to show all the activities and amenities that they can provide. There are studies to evaluate website performance based on functionality, usability and other factors; nevertheless, they are not exhaustive. This paper presents a framework for the characterization of hotel and resort websites, that was applied as a case study to the websites of 5-star hotels and resorts that operate in the tourism region of the Algarve, Portugal. This framework allows us to identify a set of features for the hotel and resort websites characterization. From these features, we propose a set of comprehensive indicators, grouping them into ten fundamental information dimensions, as well as the application of these indicators and information dimensions, which allows obtaining quantitative and qualitative results.

Socio-economic characterization of olive groves in a region in the south of Portugal

Serpa and Moura region, in the South of Portugal, have important agricultural potential as well as important agricultural resources that can and should be used and developed, in spite of economic and social depression signs reinforced by demographic difficulties that can lead to desertification in some areas. The main farms' Technical-Economic Orientation (TEO) is olives production, to produce olive oil, since this region has excellent conditions for the activity development. Some mobilizations traditionally made in olive groves lead to soil erosion and make the farm vehicles mobility much more difficult, namely those used in cultural operations. The maintenance of a soil cover in the olive grove space between lines is good, both because it favours the vehicles mobility, it promotes the rain water infiltration and, last but not the least, the soil airing. The objective of this work has been to make a socio-economic characterization of a zone of Serpa and Moura Councils in which a project of soil covers under olive trees is being developed. At the same time cultural accounts were made for the different situations under the study - irrigated olive grove with spontaneous vegetation in the space between lines, irrigated olive grove with seeded vegetation in the space between lines, irrigated olive grove with herbicide application in the space between lines, dry olive grove with spontaneous vegetation in the space between lines, dry olive grove with seeded vegetation in the space between lines and dry olive grove with herbicide application in the space between lines. The survey shows farmers in this area are younger then usual, although they don't have a high level of formal education, general or specific in agriculture. Farm areas are usually high, which can be determinant for the proposed technology adoption. Installation and operation costs for the olive grove are higher on the irrigated olive grove, but of course expected productions are also higher. For this production technology profits coming from olives production are higher then the operation costs. However, in the dry olive grove technology costs are always higher then the profits, being profitability only due to subsidies. The importance of soil cover maintenance, in this region, beside the benefits in what concerns erosion, infiltration and soil airing, is also due to its capacity to minimize the risk farmer's face.