1 resultado para sentiment burst
em Research Open Access Repository of the University of East London.
Filtro por publicador
- Aberdeen University (4)
- Aberystwyth University Repository - Reino Unido (2)
- Academic Archive On-line (Stockholm University; Sweden) (1)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (5)
- Andina Digital - Repositorio UASB-Digital - Universidade Andina Simón Bolívar (1)
- Aquatic Commons (7)
- Archive of European Integration (1)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (4)
- Aston University Research Archive (30)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (2)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (18)
- Biblioteca Digital de la Universidad Católica Argentina (2)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (6)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (25)
- Boston University Digital Common (7)
- Brock University, Canada (6)
- Bulgarian Digital Mathematics Library at IMI-BAS (1)
- CaltechTHESIS (7)
- Cambridge University Engineering Department Publications Database (8)
- CentAUR: Central Archive University of Reading - UK (58)
- Center for Jewish History Digital Collections (1)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (25)
- Cochin University of Science & Technology (CUSAT), India (3)
- CORA - Cork Open Research Archive - University College Cork - Ireland (3)
- Dalarna University College Electronic Archive (4)
- Deakin Research Online - Australia (67)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (2)
- Digital Archives@Colby (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (6)
- DigitalCommons@The Texas Medical Center (1)
- DRUM (Digital Repository at the University of Maryland) (1)
- Duke University (7)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (2)
- Gallica, Bibliotheque Numerique - Bibliothèque nationale de France (French National Library) (BnF), France (11)
- Helda - Digital Repository of University of Helsinki (13)
- Indian Institute of Science - Bangalore - Índia (68)
- Institutional Repository of Leibniz University Hannover (1)
- Instituto Politécnico do Porto, Portugal (2)
- Lume - Repositório Digital da Universidade Federal do Rio Grande do Sul (1)
- Massachusetts Institute of Technology (3)
- Ministerio de Cultura, Spain (1)
- National Center for Biotechnology Information - NCBI (12)
- Portal de Revistas Científicas Complutenses - Espanha (2)
- Publishing Network for Geoscientific & Environmental Data (1)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (79)
- Queensland University of Technology - ePrints Archive (76)
- RDBU - Repositório Digital da Biblioteca da Unisinos (3)
- ReCiL - Repositório Científico Lusófona - Grupo Lusófona, Portugal (2)
- Repositório Alice (Acesso Livre à Informação Científica da Embrapa / Repository Open Access to Scientific Information from Embrapa) (1)
- Repositorio de la Universidad de Cuenca (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (5)
- Repositório Institucional da Universidade de Aveiro - Portugal (3)
- Repositorio Institucional de la Universidad Pública de Navarra - Espanha (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (23)
- Research Open Access Repository of the University of East London. (1)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (6)
- SAPIENTIA - Universidade do Algarve - Portugal (4)
- Savoirs UdeS : plateforme de diffusion de la production intellectuelle de l’Université de Sherbrooke - Canada (8)
- School of Medicine, Washington University, United States (2)
- Universidad de Alicante (5)
- Universidad del Rosario, Colombia (5)
- Universidad Politécnica de Madrid (9)
- Universidade de Lisboa - Repositório Aberto (4)
- Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) (2)
- Universidade Federal do Rio Grande do Norte (UFRN) (5)
- Universitat de Girona, Spain (3)
- Université de Lausanne, Switzerland (6)
- Université de Montréal (3)
- Université de Montréal, Canada (205)
- Université Laval Mémoires et thèses électroniques (2)
- University of Michigan (44)
- University of Queensland eSpace - Australia (4)
- University of Southampton, United Kingdom (2)
- WestminsterResearch - UK (4)
- Worcester Research and Publications - Worcester Research and Publications - UK (2)
Resumo:
The aim of this study was to design a controlled release vehicle for insulin to preserve its stability and biological activity during fabrication and release. A modified, double emulsion, solvent evaporation, technique using homogenisation force optimised entrapment efficiency of insulin into biodegradable nanoparticles (NP) prepared from poly (dl-lactic-co-glycolic acid) (PLGA) and its PEGylated diblock copolymers. Formulation parameters (type of polymer and its concentration, stabiliser concentration and volume of internal aqueous phase) and physicochemical characteristics (size, zeta potential, encapsulation efficiency, in vitro release profiles and in vitro stability) were investigated. In vivo insulin sensitivity was tested by dietinduced type II diabetic mice. Bioactivity of insulin was studied using Swiss TO mice with streptozotocin-induced type I diabetic profile. Insulin-loaded NP were spherical and negatively charged with an average diameter of 200–400 nm. Insulin encapsulation efficiency increased significantly with increasing ratio of co-polymeric PEG. The internal aqueous phase volume had a significant impact on encapsulation efficiency, initial burst release and NP size. Optimised insulin NP formulated from 10% PEG-PLGA retained insulin integrity in vitro, insulin sensitivity in vivo and induced a sustained hypoglycaemic effect from 3 hours to 6 days in type I diabetic mice.