1 resultado para Role-play
em Research Open Access Repository of the University of East London.
Filtro por publicador
- KUPS-Datenbank - Universität zu Köln - Kölner UniversitätsPublikationsServer (1)
- Repository Napier (2)
- Academic Archive On-line (Stockholm University; Sweden) (2)
- Acceda, el repositorio institucional de la Universidad de Las Palmas de Gran Canaria. España (5)
- Adam Mickiewicz University Repository (5)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (5)
- AMS Tesi di Laurea - Alm@DL - Università di Bologna (2)
- Aquatic Commons (6)
- ArchiMeD - Elektronische Publikationen der Universität Mainz - Alemanha (3)
- Archive of European Integration (7)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (7)
- Aston University Research Archive (32)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (11)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (12)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (1)
- Blue Tiger Commons - Lincoln University - USA (1)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (57)
- Boston University Digital Common (1)
- Brock University, Canada (14)
- Bucknell University Digital Commons - Pensilvania - USA (5)
- CaltechTHESIS (3)
- Cambridge University Engineering Department Publications Database (2)
- CentAUR: Central Archive University of Reading - UK (58)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (16)
- Cochin University of Science & Technology (CUSAT), India (4)
- Comissão Econômica para a América Latina e o Caribe (CEPAL) (5)
- CORA - Cork Open Research Archive - University College Cork - Ireland (11)
- Corvinus Research Archive - The institutional repository for the Corvinus University of Budapest (4)
- Dalarna University College Electronic Archive (1)
- Deakin Research Online - Australia (124)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (2)
- Digital Archives@Colby (1)
- Digital Commons @ DU | University of Denver Research (1)
- Digital Commons at Florida International University (16)
- Digital Peer Publishing (1)
- DigitalCommons - The University of Maine Research (1)
- DigitalCommons@The Texas Medical Center (36)
- DigitalCommons@University of Nebraska - Lincoln (2)
- Doria (National Library of Finland DSpace Services) - National Library of Finland, Finland (2)
- DRUM (Digital Repository at the University of Maryland) (3)
- Duke University (10)
- Ecology and Society (2)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (4)
- Glasgow Theses Service (5)
- Greenwich Academic Literature Archive - UK (3)
- Helda - Digital Repository of University of Helsinki (18)
- Indian Institute of Science - Bangalore - Índia (32)
- Instituto Gulbenkian de Ciência (1)
- Instituto Politécnico do Porto, Portugal (3)
- Instituto Superior de Psicologia Aplicada - Lisboa (2)
- Massachusetts Institute of Technology (2)
- Ministerio de Cultura, Spain (5)
- National Center for Biotechnology Information - NCBI (52)
- Nottingham eTheses (2)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (2)
- QSpace: Queen's University - Canada (4)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (65)
- Queensland University of Technology - ePrints Archive (120)
- Repositório Científico da Universidade de Évora - Portugal (3)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (1)
- Repositório digital da Fundação Getúlio Vargas - FGV (2)
- Repositório Institucional da Universidade de Aveiro - Portugal (1)
- Repositório Institucional da Universidade Federal do Rio Grande do Norte (1)
- Repositorio Institucional de la Universidad de Málaga (1)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (46)
- Research Open Access Repository of the University of East London. (1)
- Royal College of Art Research Repository - Uninet Kingdom (2)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (4)
- School of Medicine, Washington University, United States (2)
- Scielo España (1)
- The Scholarly Commons | School of Hotel Administration; Cornell University Research (1)
- Universidad de Alicante (2)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (4)
- Universidade Complutense de Madrid (1)
- Universidade de Lisboa - Repositório Aberto (3)
- Universidade Federal do Pará (1)
- Universidade Federal do Rio Grande do Norte (UFRN) (3)
- Universidade Metodista de São Paulo (1)
- Universitat de Girona, Spain (2)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (3)
- Université de Lausanne, Switzerland (6)
- Université de Montréal (3)
- Université de Montréal, Canada (15)
- University of Connecticut - USA (3)
- University of Michigan (2)
- University of Queensland eSpace - Australia (20)
- University of Southampton, United Kingdom (2)
- University of Washington (3)
- WestminsterResearch - UK (6)
- Worcester Research and Publications - Worcester Research and Publications - UK (1)
Resumo:
Morphogens are signalling molecules that play a significant role in modulation of cell fate and development. Hedgehog proteins (Hh) are morphogens that have been shown to be involved in the development of immune cells. In this study, it is demonstrated that treatment of B cells with rShh, can increase B cell activation and also promote survival of B cells at 18hours post-stimulus. Also, at this time point, there was found to be an increase in secretion of antibody isotypes and IL-6. By 40hours post-stimulus, it was observed that the level of B cell activation was apparently arrested in treated B cells, whereas the level of activation continued to rise in untreated B cells. Interestingly, it was observed that there was an increase in the percentage of; CD23-CD25+ B cells when B cells were treated with rShh and this was accompanied by an increase in apoptosis. Consistent with this finding in relation to apoptosis, there was an increased expression of the pro-apoptotic protein Bnip3 in B cells treated with rShh by 40hours post-stimulus. It was observed that there were three subsets of B cells arising in our culture at 40hours, which were all found to possess different characteristics. It was demonstrated that treatment with rShh can increase B cell differentiation towards FO-I at 18hours post-stimulus. By 40hours post-stimulus, Hh signalling can divert differentiation away from the FO-I B cell towards the T2-MZP, which was accompanied by an increase in IL-10 secretion. Gene expression analysis revealed that Hh signalling could modulate a number of molecules involved in delivering the BCR signal into the cells such as Btk, Nfatc1 and Traf2. Additionally, deletion of Dhh, showed that there was a skewed peripheral B cell development in the Dhh-/- mice. Overall, our data demonstrate that Hh signalling can regulate the development of B cells in response to an activation stimulus by strengthening the BCR signalling pathway.