Multi-scale dynamics on the Eastern Brazilian margin

Este trabalho combina esforços de simulação numérica e de análise de dados para investigar a dinâmica em diversos compartimentos (oceano aberto, plataforma continental e zona costeira-estuarina) e, em multiplas escalas, na Margem Continental Leste Brasileira (MCLB). A circulação de largo e mesoescala espacial e a propagação da maré barotrópica são investigadas através de uma configuração aninhada do modelo numérico ROMS. O estudo da dinâmica regional da Baía de Camamu (CMB) baseia-se na análise de dados locais. A MCLB, localizada a SW do Atlântico Sul entre 8±S e 20±S, possui plataforma estreita, batimetria complexa, e baixa produtividade primária. A sua dinâmica é influenciada pela divergência da Corrente Sul Equatorial (CSE). As simulações refletem as conexões sazonais e espaciais entre a Corrente do Brasil e a Contra Corrente Norte do Brasil , em conexão com a dinâmica da CSE. As simulações revelam atividades vorticais nas proximidades da costa e interações com a dinâmica costeira, cujos padrões são descritos. A validação do modelo em mesoescala é baseada em cálculos de energia cinética turbulenta e em dados históricos de transporte. A CMB, localizada a 13±400S, abriga uma comunidade piscatória tradicional e extenso de manguezal. Situa-se porém sobre uma bacia sedimentar com grande reservas de óleo e gás, estando em tensão permanente de impacto ambiental. Neste trabalho sumarizamos as condições físicas regionais e investigamos sua dinâmica interna, focando sua variabilidade em amostragens realizadas sob condições de seca (Setembro de 2004) e de chuva (Julho de 2005). Finalmente, o modelo numérico ROMS é forçado com o sinal de maré, empregando-se uma configuração simples (com coeficientes de atrito de fundo constantes e condições hidrográficas homogéneas), com o intuito de avaliar sua resposta e investigar a natureza da propagação da maré barotrópica na MCLB, convergindo na CMB. A análise da resposta do modelo à maré basea-se em séries históricas do nível do mar para a MCLB e dados recentes da CMB.

Cooperative communications in multi-rate IEEE 802.11 networks

Esta tese apresenta um estudo sobre alguns dos protocolos de cooperação MAC para redes sem fios utilizando o sistema IEEE 802.11 multi-débito. É proposto um novo modelo de arquitetura para a categorização e análise da cooperação em redes sem fios, tendo este modelo sido aplicado a protocolos cooperativos existentes para camada MAC. É investigado como as características do meio físico, assim como os requisitos de níveis superiores podem ser aplicados ao processo de cooperação, com vista a melhorar as características de funcionamento da rede de comunicações. Para este propósito são exploradas as métricas mais relevantes para o processo de cooperação. São igualmente estudados os limites impostos pelos protocolos da camada MAC e as limitações práticas impostas por protocolos da família de normas que compõem o IEEE 802.11. Neste trabalho foi criada uma métrica multicamada, que permite considerar os requisitos aplicacionais de performance e o tipo de tráfego, assim como a mobilidade dos dispositivos, no funcionamento dos mecanismos de cooperação. Como forma de validação, e para corretamente avaliar o impacto da métrica, um novo protocolo de cooperação foi desenvolvido e implementado. O seu funcionamento é descrito de forma analítica assim como validado através de a um ambiente de simulação. Os resultados obtidos mostram que a utilização de uma métrica multicamada é uma técnica robusta, fornecendo melhorias consistentes no contexto de redes IEEE 802.11. São igualmente demonstradas várias outras características de funcionamento com impacto para as comunicações. Estes dados fornecem uma visão real e encorajadora para a realização de mais pesquisas para a melhoria da performance dos protocolos cooperativos, assim como a sua utilização num variado número de aplicações futuras. No final do documento são apresentados alguns desafios para a continuação da investigação deste tópico.

Multi-standard reconfigurable wireless transmitter architectures

The continuous demand for highly efficient wireless transmitter systems has triggered an increased interest in switching mode techniques to handle the required power amplification. The RF carrier amplitude-burst transmitter, i.e. a wireless transmitter chain where a phase-modulated carrier is modulated in amplitude in an on-off mode, according to some prescribed envelope-to-time conversion, such as pulse-width or sigma-delta modulation, constitutes a promising architecture capable of efficiently transmitting signals of highly demanding complex modulation schemes. However, the tested practical implementations present results that are way behind the theoretically advanced promises (perfect linearity and efficiency). My original contribution to knowledge presented in this thesis is the first thorough study and model of the power efficiency and linearity characteristics that can be actually achieved with this architecture. The analysis starts with a brief revision of the theoretical idealized behavior of these switched-mode amplifier systems, followed by the study of the many sources of impairments that appear when the real system is implemented. In particular, a special attention is paid to the dynamic load modulation caused by the often ignored interaction between the narrowband signal reconstruction filter and the usual single-ended switched-mode power amplifier, which, among many other performance impairments, forces a two transistor implementation. The performance of this architecture is clearly explained based on the presented theory, which is supported by simulations and corresponding measured results of a fully working implementation. The drawn conclusions allow the development of a set of design rules for future improvements, one of which is proposed and verified in this thesis. It suggests a significant modification to this traditional architecture, where now the phase modulated carrier is always on – and thus allowing a single transistor implementation – and the amplitude is impressed into the carrier phase according to a bi-phase code.

The effects of chemicals in isopods : a multi-organizational evaluation

The global aim of this thesis was to evaluate and assess the effects of a pesticide (dimethoate) and a metal (nickel), as model chemicals, within different organization levels, starting at the detoxification pathways (enzymatic biomarkers) and energy costs associated (energy content quantification, energy consumption and CEA) along with the physiological alterations at the individual and population level (mortality), leading to a metabolomic analysis (using liquid 1H-NMR) and finally a gene expression analysis (transcriptome and RT-qPCR analysis). To better understand potential variations in response to stressors, abiotic factors were also assessed in terrestrial isopods such as temperature, soil moisture and UV radiation. The evaluation performed using biochemical biomarkers and energy related parameters showed that increases in temperature might negatively affect the organisms by generating oxidative stress. It also showed that this species is acclimated to environments with low soil moisture, and that in high moisture scenarios there was a short gap between the optimal and adverse conditions that led to increased mortality. As for UV-R, doses nowadays present have shown to induce significant negative impact on these organisms. The long-term exposure to dimethoate showed that besides the neurotoxicity resulting from acetylcholinesterase inhibition, this stressor also caused oxidative stress. This effect was observed for both concentrations used (recommended field dose application and a below EC50 value) and that its combination with different temperatures (20ºC and 25ºC) showed different response patterns. It was also observed that dimethoate’s degradation rate in soils was higher in the presence of isopods. In a similar study performed with nickel, oxidative stress was also observed. But, in the case of this stressor exposure, organisms showed a strategy where the energetic costs necessary for detoxification (biomarkers) seemed to be compensated by positive alterations in the energy related parameters. In this work we presented for the first time a metabolomic profile of terrestrial isopods exposed to stressors (dimethoate and niquel), since until the moment only a previous study was performed on a metabolomic evaluation in nonexposed isopods. In the first part of the study we identify 24 new metabolites that had not been described previously. On the second part of the study a metabolomic profile variation of abstract non-exposed organism throughout the exposure was presented and finally the metabolomic profile of organisms exposed to dimethoate and nickel. The exposure to nickel suggested alteration in growth, moult, haemocyanin and glutathione synthesis, energy pathways and in osmoregulation. As for the exposure to dimethoate alterations in osmoregulation, energy pathways, moult and neurotransmission were also suggested. In this work it was also presented the first full body transcriptome of a terrestrial isopod from the species Porcellionides pruinosus, which will complement the scarce information available for this group of organisms. This transcriptome also served as base for a RNA-Seq and a RT-qPCR analysis. The results of the RNA-Seq analysis performed in organisms exposed to nickel showed that this stressor negatively impacted at the genetic and epigenetic levels, in the trafficking, storage and elimination of metals, generates oxidative stress, inducing neurotoxicity and also affecting reproduction. These results were confirmed through RT-qPCR. As for the impact of dimethoate on these organisms it was only accessed through RT-qPCR and showed oxidative stress, an impact in neurotransmission, in epigenetic markers, DNA repair and cell cycle impairment. This study allowed the design of an Adverse Outcome Pathway draft that can be used further on for legislative purposes.